美国黑豚养殖技术

美国黑豚属野生皮毛兽种类,是食草哺乳动物,形态小而肥胖,通身黑色与兔十分相似,成龄黑豚体重一般在800g左右,最重可达到1500-2000g,前肢短小,不善跳跃,行动敏捷。皮毛丰厚细密、黑亮光涌,是加工高档裘皮服装和工艺饰品的加工原料,豚肉是味道鲜美的上等佳品,其血液是提取歧氧化酶的最理想原料。具有很高的经济值和利用价值。     

类固醇合成相关酶在小鼠不同组织中的表达

利用RT-PCR和Western-blotting技术分析了胆固醇侧链裂解酶（P450scc）、3β-羟甾脱氢酶（3β-HSD）、C17-20裂解酶（P450c17）、StAR mRNA和蛋白在成年昆明小白鼠心脏、脾、肝、肾中的表达.结果显示,StAR mRNA及其蛋白、P450scc mRNA在以上组织中均有表达;3β-HSD在肾中有较强的表达,在其他组织的表达较弱;P450c17在上述组织中无明显的表达,但在睾丸中有明显的表达.这表明,心脏、脾、肝、肾均具有合成类固醇的能力,但合成类固醇的类型存在一定的差异.     

大众化教育背景下高等教育定位的思索

本文分析了传统高等教育定位与高等教育现状的种种不适应情况，提出了在大众化教育背景下应建立高等教育的新定位。从教育主体、教育理念、发展目标、人才培养、教育模式和教育政治关系等方面探讨高等教育的新定位，明确指出新世纪高等教育的任务与责任。     

退化草地土壤生化活性研究

通过对三种不同退化程度的退化草地土壤生化活性进行测定和相关性分析，结果表明草地退化后其土壤生化活性受到不同程度的影响，表现为土壤生化活性随着退化程度增高而减小，但差异并非都达到统计学上的显著性，而土壤生化活性间的相关性则显著地与草地退化程度有关，说明退化草地土壤微生物间的协调作用受到显著影响，从而影响土壤氮素和碳素代谢途径。     

Determination of sulfamethazine and trimethoprim in liquid feed premixes by HPLC and diode array detection, with an analysis of the uncertainty of the analytical results.

Sulfamethazine (SMZ) and trimethoprim (TMP) are antibacterials used in veterinary practice. This paper describes a method for their determination in veterinary liquid feed premixes that is based on liquid chromatography with diode array detection. Gradient elution with methanol and ammonium acetate achieved excellent separation of the two analytes within 15 min without any interference from the matrix. Absorbance of the column effluent was monitored at 264 nm for SMZ and at 230 nm for TMP. Detailed analyses of the uncertainties of determinations afford estimated expanded uncertainties of, respectively, 0.2 and 0.1 w/v % for typical SMZ and TMP concentrations of 10.7 and 2.1 w/v %, respectively. At the lower end of the calibrated range of the method, the dominant source of uncertainty is the preparation of standards and the construction of the calibration line.     

规范科技论文写作提高投稿的命中率

根据编辑学、语法修辞学理论,针对科技论文手稿中在论文结构、语言表达等方面存在的比较普遍的问题进行分析,并明确规范用法,旨在使科技论文作者撰写论文时注意养成良好的写作习惯,不断提高写作水平,从而提高论文质量,提高投稿命中率.     

Laboratory-based surveillance of salmonellosis of humans in Germany--safety of Salmonella typhimurium and Salmonella enteritidis live vaccines]

The Paul Ehrlich Institute, Langen, in Germany has been licensed different live vaccines of S. Typhimurium and S. Enteritidis for use in the veterinary medicine since I the 90s. The Robert Koch Institute has established a lab-based surveillance system for these live vaccine strains for an evaluation of recent public health safety. Since 2000 all strains of S. Typhimurium and S. Enteritidis from humans were investigated in respect to their phage types and other vaccine markers. 3676 S. Typhimurium strains and 4489 S. Enteritidis strains mainly from Salmonellose patients were investigated after phage typing according to their auxotrophic or antibiotic resistance markers. The live vaccine strains of Zoosaloral, TAD Salmonella vacT or TAD Salmonella vacE and Salmovac SE could not be found from infections in humans.     

The effect of eradication of lice on the occurrence of the grain defect light flecks and spots on cattle hides.

The influence of an eradication programme for lice on the prevalence of light flecks and spots on cattle hides was studied in 33 dairy cattle herds during a period of two and a half years. Lice were eradicated from the main group of herds after 9 to 12 months and the quality of the hides before and after treatment was compared. Hides from slaughtered animals were collected during the study period, tanned and examined with special emphasis on the occurrence of the grain damage light flecks and spots. The prevalence of hides without light flecks and spots increased from 24.2% before treatment to 61.6% after treatment. The prevalence of hides free from the damage increased significantly in all examined anatomical regions. The improvement in hide quality was most marked in the shoulders and neck region which corresponded to the major predilection site of cattle lice. The prevalence of hides with light flecks and spots started to decrease in the first period (2-40 days) after eradication. The changes after treatment suggested that most healing process took place over a period of about 4 months. The eradication programme eliminated the seasonal variation in the prevalence of light flecks and spots which was present before treatment.     

Effect of concentrate crude protein level on grass silage intake, milk yield and nutrient utilisation by dairy cows in early lactation.

Twenty-one multiparous dairy cows were fed concentrates containing three levels (119, 154 and 191 g/kg DM) of crude protein (CP) during the first ten weeks of lactation. Part of the grain and molassed sugar beat pulp was substituted with 0% (RSM0), 15% (RSM15) or 30% (RSM30) repeseed meal. Wilted grass silage was fed ad libitum after calving. The average response between RSM0 and RSM15 was +1.66 kg milk/d per percentage unit change in concentrate CP content. No further response occurred between RSM15 and RSM30. The positive effect of RSM inclusion was seen throughout the experimental period and was associated with increased plasma non esterified fatty acids (NEFA) and decreased plasma insulin concentration one week after calving, and higher efficiency of metabolisable energy utilisation for milk production. Digestibility of the diet remained unaffected. Milk and plasma urea tended to increase with RSM30 indicating excessive supply of rumen degradable protein. Because of the limited potential of cows to compensate for a deficit in feed protein supply by mobilising tissue protein, a substantial milk yield response can be achieved with a moderate level of protein supplementation during early lactation.     

基于ASP.NET的造林专家系统

基于ASP NET的设计思想和实现方法,采用知识库和专家系统原理构建造林专家系统。利用该系统辅助解决造林体系中的非结构化问题,为其规划设计的论证决策提供有力工具及手段。