Mitigation of greenhouse gas emissions from soil under silage production by use of organic manures or slow-release fertilizer

Abstract. Grassland is a major source of nitrous oxide (N₂O) and methane (CH₄) emissions in the UK, resulting from high rates of fertilizer application. We studied the effects of substituting mineral fertilizer by organic manures and a slow-release fertilizer in silage grass production on greenhouse gas emissions and soil mineral N content in a three-year field experiment. The organic manures investigated were sewage sludge pellets and composted sewage sludge (dry materials), and digested sewage sludge and cattle slurry (liquid materials). The organic manures produced N₂O and carbon dioxide (CO₂) consistently from time of application up to harvest. However, they mitigated N₂O emissions by around 90% when aggregate emissions of 15.7 kg N ha⁻¹ from NPK fertilizer were caused by a flux of up to 4.9 kg N ha⁻¹ d⁻¹ during the first 4 days after heavy rainfall subsequent to the NPK fertilizer application. CH₄ was emitted only for 2 or 3 days after application of the liquid manures. CH₄ and CO₂ fluxes were not significantly mitigated. Composting and dried pellets were useful methods of conserving nutrients in organic wastes, enabling slow and sustained release of nitrogen. NPK slow-release fertilizer also maintained grass yields and was the most effective substitute for the conventional NPK fertilizer for mitigation of N₂O fluxes.     

Soil and residue management effects on arable cropping conditions and nitrous oxide fluxes under controlled traffic in Scotland 1. Soil and crop responses

Soil compaction can affect crop growth and greenhouse gas emission and information is required of how both these aspects are affected by compaction intensity and weather. In this paper we describe treatments of compaction intensity and their effects on soil physical conditions and crop growth in loam to sandy loam cambisol soils. Soil conditions and crop performance were measured over three seasons in a field experiment on soil compacted by wheels on freshly ploughed seedbeds. Ploughing buried the chopped residues of the previous crop. After ploughing, traffic was controlled such that the experimental plots received wheel traffic only as treatments. The overall objective was to discover how the intensity and distribution of soil compaction just before sowing influenced crop performance, soil conditions and emissions of nitrous oxide. Compaction treatments were zero, light compaction by roller (up to 1 Mg m⁻¹) and heavy compaction by loaded tractor, (up to 4.2 Mg). The experiment was located at Boghall, near Edinburgh (860 mm average annual rainfall) for the first two seasons under spring and winter barley (Hordeum vulgare L.) and in a drier area at North Berwick (610 mm average annual rainfall) for the third season under winter oil-seed rape (Brassica napus L.). Heavy compaction in dry soil conditions had little effect on crop growth. However, in wet conditions heavy compaction reduced air porosity, air permeability and gas diffusivity, increased cone resistance and limited winter barley growth and grain yield. Heavy compaction in wet conditions reduced winter barley yields to 7.1 Mg ha⁻¹, in comparison to 8.8 Mg ha⁻¹ in the zero compaction treatment. The compaction status of the top 15 cm of soil seemed to be particularly important. Loosening of the top 10 cm of soil immediately after heavy compaction restored soil conditions for crop growth. However, zero seed bed compaction gave patchy and uneven crop emergence in dry conditions. Both zero and light compaction to a target depth of 10 cm gave similar crop productivity. Maintenance of a correct compaction level near the soil surface is particularly important for establishment and overwintering of barley and oil seed rape.     

Considerations for Scottish soil monitoring in the European context

Two contrasting phases of work are described that help inform the development and requirements of a soil monitoring system: firstly, the development and application of a multi-criterion analysis of soil quality indicators grounded in the basic natural sciences; and secondly, scrutiny of the outcome of that process by a wide range of non-specialist but key stakeholders at a workshop. This process ensures that the final monitoring design meets both the scientific rigour expected from a monitoring system and as far as possible meets the aspirations of policy and regulatory stakeholders. Individual indicators of soil quality were evaluated in terms of their applicability against a number of important environmental and logistical parameters and therefore their overall fitness for purpose. These included relevance to different soil types, functions, habitats and threats to soil, the inherent variability of soil, and a range of technical aspects such as analytical complexity, precision and reproducibility of analytical results and whether a standard operating procedure (SOP) existed for the technique. A tiered approach to soil monitoring was supported by workshop delegates. This will require indicators that are suitable and effective at national, site-specific and process-level scales. In addition, the opportunities for synchronizing soil monitoring with air and water quality monitoring should be considered and the potential for integrating on-site measurements with remote methods should be researched further. It was considered by workshop attendees that soil monitoring should be rooted in pedological principles (i.e. recognizing defined soil horizons) to ensure that results can be extrapolated from individual sites and to retain flexibility.     

Soil and residue management effects on cropping conditions and nitrous oxide fluxes under controlled traffic in Scotland2. Nitrous oxide, soil N status and weather

Nitrogen from fertilisers and crop residues can be lost as nitrous oxide (N₂O), a greenhouse gas that causes an increase in global warming and also depletes stratospheric ozone. Nitrous oxide emissions, soil chemical status, temperature and N₂O concentration in the soil atmosphere were measured in a field experiment on soil compaction in loam and sandy loam (cambisols) soils in south-east Scotland. The overall objective was to discover how the intensity and distribution of soil compaction by tractor wheels or by roller just before sowing influenced crop performance, soil conditions and production and emissions of N₂O under controlled traffic conditions. Compaction treatments were zero, light compaction by roller (up to 1 Mg per metre of length) and heavy compaction by loaded tractor (up to 4.2 Mg). In this paper we report the effects on production and emissions of N₂O and relate them to soil and crop conditions. Nitrous oxide fluxes were substantial only when the soil water content was high (>27 g per 100 g). Fertiliser application stimulated emissions in the spring whereas crop residues stimulated emissions in autumn and winter. Heavy compaction increased N₂O emissions after fertiliser application or residue incorporation more than light or zero compaction. The bulk densities of the heavily and lightly compacted soils were up to 89% and 82% of the theoretical (Proctor) maxima. Higher soil cone resistances, temperatures and nitrogen availability and lower gas diffusivities and air-filled porosities combined to make the heavily compacted soil more anaerobic and likely to denitrify than the zero or lightly compacted soil. Compaction sufficient to increase N₂O emissions significantly corresponded with adverse soil conditions for winter barley (Hordeum vulgare L.) growth. Soil tillage, which ensures that soil compaction is no greater than in our light treatment and is confined to near the soil surface, may help to mitigate both surface fluxes of N₂O and losses to the subsoil.     

Soil fauna alter the effects of litter composition on nitrogen cycling in a mineral soil

Plant chemical composition and the soil community are known to influence litter and soil organic matter decomposition. Although these two factors are likely to interact, their mechanisms and outcomes of interaction are not well understood. Studies of their interactive effects are rare and usually focus on carbon dynamics of litter, while nutrient dynamics in the underlying soil have been ignored. A potential mechanism of interaction stems from the role fauna plays in regulating availability of litter-derived materials in the mineral soil. We investigated the role of soil fauna (meso, macro) in determining the effect of surface-litter chemical composition on nitrogen mineralization and on the micro-food web in mineral soils. In a field setting we exposed mineral soil to six types of surface-applied litter spanning wide ranges of multiple quality parameters and restricted the access of larger soil animals to the soils underlying these litters. Over six months we assessed litter mass and nitrogen loss, nitrogen mineralization rates in the mineral soils, and soil microbes and microfauna. We found evidence that the structure of the soil community can alter the effect of surface-litter chemical composition on nitrogen dynamics in the mineral soil. In particular, we found that the presence of members of the meso- and macrofauna can magnify the control of nitrogen mineralization by litter quality and that this effect is time dependent. While fauna were able to affect the size of the micro-food web they did not impact the effect of litter composition on the abundance of the members of the micro-food web. By enhancing the strength of the impact of litter quality on nitrogen dynamics, the larger fauna can alter nitrogen availability and its temporal dynamics which, in turn, can have important implications for ecosystem productivity. These findings contribute to evidence demonstrating that soil fauna shape plant litter effects on ecosystem function.     

Straw incorporation and tillage for winter barley: Soil structural effects

A large-scale field experiment was conducted over four seasons on a gleysol (24% clay in topsoil) in Scotland. Conventional ploughing, shallow ploughing and shallow rotary or tine cultivation were investigated for the incorporation of straw in winter barley. Straw after harvest was either chopped or removed. Thus the residue treatments were either straw plus stubble or stubble only. Incorporation depths ranged from 0–100 to 0–300 mm.The presence of straw changed soil physical conditions after several seasons in which straw was incorporated. In the ploughed treatments, the presence of straw plus stubble decreased the average soil water content and matric potential in the topsoil in comparison to stubble only, indicating more rapid drainage. This was associated with the presence of buried straw in zones of loose soil just above plough depth and with a long-term increase in soil pore continuity related to the presence of straw residues. The presence of straw plus stubble under conventional ploughing decreased thermal diffusivities in the top 150 mm of soil in comparison with stubble only. Shallow incorporation of straw plus stubble gave higher overall strengths, bulk densities, lower water infiltration rates and poorer drainage in the topsoil than deeper incorporation. However, shallow incorporation caused an accumulation of organic matter over the first three seasons. This accumulation probably contributed to the increased aggregate stability and resistance to compaction of the top 50-mm soil layer, indicating improved resistance to erosion and to further compaction.     

Isolation of mycoplasmas from bovine semen in Northern Ireland

In a survey of 332 fresh and 137 processed bovine semen samples and 25 preputial washes, mycoplasmas and, or, ureaplasmas were isolated from 46 per cent, 31 per cent and 80 per cent, respectively. Intermittent isolation from different semen collections from the same bull indicated that at least three collections per bull were necessary to determine whether infection was present. When stored processed samples were examined Mycoplasma canadense and M bovigenitalium were isolated from straws taken as long ago as 1975. Addition of lincomycin and spectinomycin to the semen extender eliminated the isolation of mycoplasmas and reduced the rate of isolation of ureaplasmas.     

Experimental intrauterine inoculation of pregnant ewes with ureaplasmas

The pregnancies of 13 ewes which were inoculated intrauterine with one of two strains of ovine ureaplasmas resulted in 9 normal and 3 abnormal births, and one ewe was found to be no longer pregnant on postmortem examination. Vaginal ureaplasma infection was detected in the majority of ewes only after lambing. Of the 12 ewes examined at postmortem, ureaplasmas were isolated from the uterus of 5 out of the 6 necropsied up to 21 days post-partum. The vulvar/preputial areas of the majority of lambs that survived were infected with ureaplasmas for the duration of the experiment, but infections of the nasal cavity and eye areas, detected at birth in 4 lambs, were resolved within 8 days post-partum.The only pathological effects detected that could possibly be attributed to ureaplasma infection were a placentitis in an ewe that aborted, and the resorption of the foetus in another.     

Plant growth – resource or strategy limited: insights from responses to gibberellin

We used exogenous application of a phytohormone (gibberellin GA₃, ‘GA’) to test the hypothesis that common perennial grasses may not be growing at all times to the limit of resource availability. Plants were taken from the field in winter, and again in summer and their responses to GA assessed under standard conditions, indoors, to reveal their ‘potential’ for growth at different times. Time of year, and associated developmental state, had a major impact on the capacity of plants to respond to exogenous GA, and less so their current growing conditions, temperature and N availability, during measurement. A major increase in dry matter (DM) production in winter‐derived plants took place at both low and high N, with no evidence of a reduction in N content in tissues. That ryegrass plant growth can be stimulated, without externally adding resources, supports the hypothesis there is an element of internal control in how plants respond to ‘signals’ in their environment, that might be manipulated. This offers prospects for reducing environmental impacts (leaching, N₂O) compared with obtaining the same yield increase by adding fertilizer N in early season. Responses to exogenous GA were detected (as significant) but far smaller in summer‐derived plants. Molecular mechanisms of detection of N resource signals, developmental triggers and the role of endogenous gibberellin need to be unravelled to assess scope for breeding ryegrass germplasm to better match demands for increased production with greater resource‐use efficiency.     

Assessing serum metabolite profiles as predictors for feed efficiency in broiler chickens reared at geographically distant locations

1. The objective of this study was to investigate differences in growth performance, serum intermediary metabolites, acute-phase proteins and white blood cells in low, medium and high–residual feed intake (RFI) chickens. It was also assessed if the environment affects the feed efficiency (FE) and FE-related performance and serum profiles of chickens.

2. Individual body weight (BW) and feed intake (FI) were recorded from d 7 of life. At 5 weeks of age, female and male broiler chickens (Cobb 500) were selected according to their RFI (L1: Austria; L2: UK; n = 9/RFI group, sex and locatity -45on) and blood samples were collected.

3. Chickens at L1 had similar FI but a 15% higher BW gain compared to chickens at L2. The RFI values of female chickens were ?231, 8 and 215 g and those of male chickens ?197, 0 and 267 g for low, medium and high RFI, respectively.

4. Location affected serum glucose, urea, cholesterol, non-esterified fatty acids (NEFA) and ovotransferrin in females, and serum glucose and triglycerides in male chickens. Serum uric acid and NEFA linearly increased from low to high RFI in females, whereas in males, cholesterol showed the same linear response from low to high RFI. Serum alpha-1-acid glycoprotein and blood heterophil-to-lymphocyte ratio linearly increased by 35% and 68%, respectively, from low to high RFI but only in male chickens at L1.

5. Regression analysis showed significant positive relationships between RFI and serum uric acid (R² = 0.49) and cholesterol (R² = 0.13).

6. It was concluded that RFI-related variation in serum metabolites of chickens was largely similar for the two environments and that serum metabolite patterns could be used to predict RFI in chickens.