稀释液中添加谷氨酰胺对绒山羊冷冻精液品质的影响

在绒山羊冷冻精液稀释液中分别添加0mmol/L、2.5mmol/L、5.0mmol/L、7.5mmol/L和10.0mmol/L五个梯度的谷氨酰胺,以确定冷冻效果最好的谷氨酰胺添加浓度,优化绒山羊冷冻稀释液配方。结果表明:添加5.0mmol/L谷氨酰胺组精子的活率、顶体完整率和生存指数均极显著或显著地高于其他试验组(P0.01,P0.05),而畸形率极显著或显著低于其他试验组(P0.01,P0.05);在脂质过氧化反应和抗氧化酶活性方面,添加5.0 mmol/L组MDA浓度极显著低于其他试验组(P0.01),而CAT活性极显著高于其他试验组(P0.01)。总之,在稀释液中添加5.0mmol/L谷氨酰胺,在冷冻—解冻过程中能起到很好的保护精子的作用。     

京、津、冀区域扬沙天气气候变化特征分析

利用京、津、冀区域内所辖 6 5个气象观测站 1 95 4 - 2 0 0 2年的观测资料 ,分析了扬沙天气的气候变化特征。结果表明 :京、津、冀区域绝大部分地区是扬沙天气的主要影响区。高原、平原和丘陵地区是扬沙天气的多发区和易发区 ,宁晋、邢台一带是扬沙天气的高频区。扬沙的发生时间具有明显的季节变化特征 ;此区域内的扬沙呈现整体减少趋势 ,各分区域的变化趋势具有相似性 ,只是在减少幅度和年代际减少趋势上略有不同     

不同类型有机肥对烤烟石油醚提取物及香气物质含量的影响

采用田间试验方法,研究不同类型有机肥对石油醚提取物及香气物质含量的影响。结果表明:1)施用有机肥均能增加石油醚提取物含量,其中T3(无机化肥+芝麻饼肥+生物有机肥)处理的中、上部叶石油醚提取物含量最大,分别为7.76%、8.75%;2)施用有机肥处理对上部叶的增香效果优于中部叶,有机肥处理的上部叶有24种香气物质含量均高于CK,中部叶有18种高于CK;其中T3处理的中、上部叶致香物质总量最大,分别高达1 291.45、1 524.34μg/g,较CK处理分别增加3.32%、34.86%。     

数控线切割加工中工件表面质量的改善

文章提出了对线切割加工工件表面质量有直接影响的相关因素,并争对所涉及如:人员、设备、材料等方面的主要因素作出了具体分析及解决方案。解决了线切割加工中工件表面质量常见的问题。     

金黄色葡萄球菌α-溶血素的研究进展

目的α-溶血素在金黄色葡萄球菌(金葡菌)肺炎的感染过程中起着非常重要的作用,可作为治疗金葡菌肺炎的新靶标。因此,研究百里香酚对金葡菌α-溶血素表达的影响具有重要意义。方法测定最小抑菌质量浓度(MIC)和生长曲线并进行了溶血试验、蛋白免疫印迹分析、荧光定量PCR以及细胞毒性试验。结果百里香酚对金葡菌USA300的MIC为256 μg/mL,亚抑菌质量浓度的百里香酚(32 μg/mL)可下调金葡菌hla和RNAIII的转录水平,降低α-溶血素的表达,对溶血素介导的肺癌上皮细胞A549损伤具有保护作用。结论百里香酚具有成为新型抗金葡菌感染药物的潜力。     

Analysis on the effect of runoff retention and pollution load control for two vegetation roofs during rainfall events

In order to understand the effect of runoff retention and pollution load control for vegetation roofs during rainfall events, the evaluation criterion of vegetation roof performance is redefined and two vegetation roofs are established artificially based on the code for the design of roof greening, which is investigated during rainfall events. Results show that ophiopogon japonicus roof can delay the production of runoff by 25〖XC半字线.tif,JZ〗48 min compared to the rain occurring and retention runoff volume by 40%〖XC半字线.tif,JZ〗58%, while sambucus dhinensis roof can make the generation of runoff 60〖XC半字线.tif,JZ〗130 min later than the rain beginning and runoff volume retention can be as high as 54%〖XC半字线.tif,JZ〗80%. In addition to PO 3-4 P, the reduction of pollution load of NO -2 N, NH3 N, TP, COD, TN and NO -3 N by both vegetation roofs can reach 95.1%〖XC半字线.tif,JZ〗98.6%, 87.2%〖XC半字线.tif,JZ〗93%, 72.4%〖XC半字线.tif,JZ〗83%, 76%〖XC半字线.tif,JZ〗81.8%, 68.7%〖XC半字线.tif,JZ〗78.4% and 40.9%〖XC半字线.tif,JZ〗60.6% respectively. The level of pH, COD, NH3 N and TP in vegetation roofs runoff can meet class Ⅲ surface water standard. Meanwhile, both vegetation roofs have similar effect in neutralization acid deposition (i.e., from 5.8〖XC半字线.tif,JZ〗6.0 (in rain water) to 7.0〖XC半字线.tif,JZ〗7.6 (in eco roof runoff water)). Plants grown on vegetation roofs can influence the performance of vegetation roofs. Based on the results, ophiopogon japonicus has an advantage of sambucus chinensis in controlling phosphorus pollution, while sambucus chinensis manifests better in reducing nitrogen load. Vegetation roofs are becoming an important urban landscape more and more, and study results in this paper provide references for the improvement of vegetation roofs construction and more reasonable evaluation of their running performance.     

鸭种蛋孵化工艺的优化

1 种蛋的收集、消毒与保存 1.1 种蛋的收集 种鸭与种鸡所处的环境有所不同,鸭离不开水,因此鸭舍比鸡舍脏,种鸭一般在凌晨3:00左右产蛋,如不及时收集种蛋,易被种鸭践踏造成污染,所以收集种蛋应越早越好.在收集时应按种用蛋、非种用蛋(双黄蛋、破蛋、畸形蛋)分别装入专用鸭蛋篓.早晨6:30产蛋基本结束,第一次集蛋完成后应尽快入库.有个别的种鸭产蛋(约占母鸭群的3%左右)直到上午8:00左右产蛋才结束,应将第二次集好的种蛋再送到蛋库.     

平原沙土区不同林分类型下土壤有机碳库特征及其影响因子

目的 明晰不同林分类型下土壤有机碳库特征,以期为平原沙土区土壤碳库的提高和生态恢复提供理论依据。 方法 以江苏省平原沙土区丰县16年生杨树纯林、柳树纯林、杨柳混交林3种林分类型及相邻的撂荒地（对照）为对象,测定0～20、20～40、40～60、60～80、80～100 cm等5个土壤层次有机碳及活性组分和土壤酶活性,计算土壤碳库管理指数,并对土壤有机碳库特征和环境因子进行相关性分析。 结果 （1）3种林分类型及对照各土层有机碳含量和储量范围分别为1.03～5.88 g·kg−1和3.53～17.55 t·hm−2。与对照相比,0～100 cm土层,3种林分类型土壤有机碳含量增加了45.2%～82.2%。杨树纯林0～100 cm土层有机碳储量分别是柳树纯林、杨柳混交林和对照的1.23、1.24、1.83倍。（2）与对照相比,0～100 cm土层,3种林分类型的土壤易氧化有机碳、可溶性有机碳和微生物生物量碳含量分别提高了28.6%～48.0%、6.8%～9.7%和21.6%～33.4%。3种林分类型土壤碳库管理指数显著高于100%（P<0.05）。（3）林分类型显著影响土壤蔗糖酶和多酚氧化酶活性（P<0.05）,杨柳混交林0～100 cm土层多酚氧化酶活性显著高于杨树纯林和对照（P<0.05）。（4）相关性分析表明,土壤有机碳库特征受多酚氧化酶和β-葡萄糖苷酶活性显著影响,有机碳含量和储量、易氧化有机碳含量及碳库管理指数受蔗糖酶、过氧化物酶活性显著影响,微生物生物量碳受蔗糖酶活性显著影响。冗余分析表明,土壤碳氮比和细根生物量是土壤有机碳库特征的主要影响因素。 结论 平原沙土区营造人工林提高了土壤有机碳及活性组分和有机碳库稳定性,杨树纯林提高土壤有机碳储量效果最好。     

基于MaxEnt模型的中国豚草潜在适生区预测分析

利用MaxEnt模型预测现代和未来气候背景下豚草在中国的潜在分布格局。研究结果表明：年平均气温和年平均降水量对豚草生存的影响呈先上升后下降趋势,豚草不适于特别炎热且干旱地区生存,最干月降水量和土壤盐基饱和度对豚草生存呈正影响;未来气候变化背景下豚草的高适生区质心移动轨迹为高纬度东北方向,有待重点监测和控制。     

Endogenous hydrogen sulfide regulates oxidant/antioxidant balance in rat with chronic obstructive pulmonary disease

AIM: To investigate the role of hydrogen sulfide (H₂S) in the protection against oxidative stress in rats with chronic obstructive pulmonary disease (COPD).METHODS: The rat model of COPD was established by cigarette smoking (CS) combined with lipopolysaccharide (LPS) instillation. Thirty-two healthy male Sprague-Dawley rats were randomly divided into 4 groups: control group, CS+LPS group, CS+LPS+NaHS (H₂S donor) group and CS+LPS+PPG (DL-propargylglycine, an inhibitor of cystathionine-γ-lyase) group. After 30 days, the lung functions of the rats were measured, the histological changes of lungs were observed under light microscope and the pathological scores were calculated. The H₂S level in plasma and the protein expression of cystathionine-γ-lyase (CSE) in the lung tissues were measured. The content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and catalase (CAT) were detected to reflect oxidative stress.RESULTS: Compared with control group, the peak expiratory flow (PEF) decreased by 24% and intra-pressure (IP) increased by 66% in CS+LPS group. The pathological scores of the lung tissues also increased. Compared with CS+LPS group, no change in the lung function was observed after given NaHS or PPG, but the pathological scores decreased in CS+ LPS+ NaHS group. Compared with control group, the content of H₂S in plasma was increased by 26% on day 16. Compared with CS+LPS group, the content of H₂S in plasma of CS+LPS+PPG group was decreased by 22% after 30 days. Compared with control group, the protein expression of CSE increased, and no statistical difference among CS+LPS group, CS+ LPS+ NaHS group and CS+LPS+PPG group was observed. Compared with control group, MDA content in the lung tissues was increased by 24% in CS+LPS group, the activity of SOD was increased by 47% and the activity of CAT was increased by 52%. Compared with CS+LPS group, the MDA content in CS+LPS+NaHS group was decreased by 21%, and no statistical difference in the activity of SOD and CAT was observed. The activity of SOD decreased by 33% after given PPG.CONCLUSION: H₂S plays a role as anti-oxidant in the rats with COPD. The CSE/ H₂S pathway may be involved in the development of COPD.