水稻籽粒镉积累KASP分子标记LCd-38的开发与利用

水稻(Oryza sativaL.)是我国主粮作物之一,稻米镉污染对我国粮食安全造成一定威胁.镉低积累水稻品种的选育可有效降低水稻籽粒镉污染风险,高效准确的镉积累相关分子标记在镉低积累水稻品种选育中具有至关重要的作用.以水稻微核心种质为材料,基于籽粒镉积累关联基因的功能性SNP位点开发了KASP分子标记LCd-38.选...     

IFN-γR和ER在大鼠下丘脑中的共存

为了探讨神经-免疫-内分泌网络中生物活性物质间相互调节和平衡的关系,本试验应用免疫组化双标记法对成年SD雌性大鼠下丘脑中IFN-γ受体（IFN-γR）和雌激素受体（ER）的共表达进行了研究。结果发现,IFN-γR和ER广泛存在于大鼠下丘脑中,其中在视前交叉上核、视前内侧核、室周核、交叉上核、下丘脑前核、下丘脑内侧核、下丘脑外侧核、乳头体前背侧核、乳头体前腹侧核等13个核团中存在大量的IFN-γR和ER双标记细胞,双标记细胞约占全部阳性标记细胞的60．9％;双标记细胞胞质呈黑褐色、胞核呈棕黄色;ER单标着色细胞以神经胶质细胞居多。研究结果表明,IFN-γ和雌激素可以分别以其各自受体为介质进行信号传递和信息整合,同时也通过在同一细胞中的相互作用而参与机体的神经-免疫-内分泌调节。     

对内蒙古鄂尔多斯高原全球第1148号国际重要湿地的展望

The Taolimiao-Alashan Nur(T-A Nur),of about 10km2 a brackish lake located on the Ordos upland of western Inner Mongolia,northern China,used to be not only supporting the largest breeding colony of the Relict Gull(Larus relictus)but also being a very good sample of the carrying capacity of a semi-desert wetland locality for those waterbirds,both breeders and migrating vagrants,of the region,and,therefore,the T-A Nur was recognized,in 2002,the No.1148 Ramsar Site of the world.     

Study on Nutritional Characteristics of Cottonseed Hull as a Feed of Sheep

In this paper through digestive and metabolic experiments studied the voluntary intake and apparent digestibility of cottonseed hulls (kernel-contained CH,kernel-free CH) and cornstalk on sheep to evaluate the nutrition value and digestion characteristics of cottonseed hulls.12 of adult Xinjiang merino rams were selected and divided into two groups (n=6 each group),and on the basis of 300 g/(head·day) of mixed concentrate,fed with kernel-free CH by 400 (group 1) or 800 (group 2) g/(head·day) respectively,to estimate the digestibility of CH by difference method;As well,another 18 of adult Xinjiang merino rams were selected and divided into three groups (n=6 each group),and on the basis of 300 g/(head·day) of mixed concentrate,cafeteria feeding with kernel-contained CH (group 3),kernel-free CH (group 4) or ground cornstalks (group 5) respectively,to study the effects of three diets on the voluntary intake,digestion and metabolism of sheep.The results showed that the apparent digestibility of the DM,OM,CP,cellulose,hemicellulose and energy of kernel-free CH by sheep were 46.71%,47.37%,-53.40%,66.60%,62.48% and 42.59%,respectively.There were no significant differences in the intake of DM,OM,cellulose,hemicellulose,energy and Ca between kernel-contained CH and kernel-free CH groups (P> 0.05);Intake of CP and P in kernel-contained CH group were extremely significantly higher than that in kernel-free CH group (P< 0.01);Intake of lignin in kernel-contained CH group was extremely significantly lower than that in kernel-free CH group (P< 0.01).There were no significant differences in the apparent digestibility of DM,OM and energy between kernel-contained CH and kernel-free CH groups (P> 0.05).The apparent digestibility of cellulose and hemicellulose in kernel-free CH group were significantly and extremely significantly higher than that in kernel-contained CH group (P< 0.05;P< 0.01),respectively.The apparent digestibility of CP in kernel-contained CH group was extremely significantly higher than that in kernel-free CH group (P< 0.01).The apparent digestibility of DM,OM,CP,cellulose and energy in ground cornstalks group were extremely significantly higher than that in kernel-contained CH and kernel-free CH groups (P< 0.01),however,the apparent digestibility of Ca and P were lower than that in kernel-contained CH and kernel-free CH groups (P> 0.05).Intake and retention of nitrogen in kernel-contained CH group were extremely significantly higher than that in kernel-free CH group (P< 0.01);The was no significant difference in the retention of nitrogen between kernel-free diet group and cornstalk diet group (P> 0.05).It was concluded that CH was a kind of special forage with larger intake,higher digestibility on the fiber substances and energy,but with a serious obstruction on protein digestion;The nutritional value between kernel-free diet and cornstalk diet was almost equal,and with lower nitrogen digestibility on kernel-free diet.     

16种中药体外抗猪繁殖与呼吸综合征病毒作用的研究

To observe the antiviral action of 16 kinds of Chinese medicinal herbs (Houttuynia cordata Thunb,Flos Chrysanthemi,Flos Lonicerae Confusae,Anemarrhena asphodeloides,CassiaTwig,Herba Ephedrae,Radix Paeoniae Rubra,Bupleurum chinense,Dryopteris setosa,Folium Isatidis, Gardenia jasminoides Ellis,Mntha haplocalyx Briq,Paeonia suffruticosa Andr,Cornu Bubali, Herba Senecionis Scandentis,Radix Gentianae) against porcine reproductive and respiratory syndrome virus （PRRSV）, the blocking, inhibiting and killing roles were detected in present study in vitro. The results showed that 13 kinds of Chinese medicinal herbs had antiviral actions against PPRSV except Paeonia suffruticosa Andr, Herba Senecionis Scandentis and Folium Isatidis, and Houttuynia cordata Thunb, Flos Chrysanthemi, Anemarrhena asphodeloides, CassiaTwig and Radix Paeoniae Rubra exhibited the best antiviral actions followed by Herba Ephedrae, Bupleurum chinense and Dryopteris setosa.     

鸟氨酸脱羧酶抗酶1基因的结构和功能

鸟氨酸脱羧酶抗酶1(OAZ1)基因可通过特殊的+1移码机制翻译全长的功能蛋白.研究发现,OAZ1能与鸟氨酸脱羧酶(ODC)结合并降解ODC,负调控细胞内多胺的水平;OAZ1还能降解Cyclin D1、Cyclin E1和Smad1周期蛋白,阻滞细胞周期;此外,近年来研究表明,OAZI还具有抗肿瘤效应和调控动物繁殖的功能.抗酶抑制因子能竞争性结合ODC-OAZ1复合体中的OAZ1,从而阻止ODC降解;天门冬酰胺也能通过抑制OAZ1的翻译来调节ODC的活性.本文就OAZ1基因结构和功能的研究现状作一综述.     

湖南省山羊脑多头蚴的线粒体nad1和nad4基因的序列测定及种系发育分析

本研究旨在阐明脑多头蚴湖南分离株线粒体烟酰胺腺嘌呤二核苷酸( NADH)脱氢酶亚单位1基因(nad1)部分序列(pnad1)和烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位4基因(nad4)部分序列(pnad4)的遗传变异情况,并用pnad1和pnad4序列重构脑多头蚴与其它带科绦虫的种群遗传关系.利用聚合酶链反应(PCR)扩增脑多头蚴的pnad1和pnad4,应用ClustalX 1.81程序对序列进行比对,再用Phylip3.67程序MP法和Mage4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树,同时利用DNAstar5.0中的Megalign程序进行同源性分析.结果显示所获得的pnad1和pnad4序列长度分别均为666和887 bp,湖南分离株与已知多头带绦虫位于同一分枝.由于脑多头蚴pnad1和pnad4序列种内相对保守,种间差异较大,故均可作为种间遗传变异研究的标记,从而为脑多头蚴的分子流行病学和其相关疾病的诊断奠定基础.     

SOE PCR重构SLA-Ⅰ复合体研究

利用剪接重叠延伸PCR(SOE PCR)技术,中间加一富含甘氨酸/丝氨酸的linker(G4S)3,将SLA-Ⅰ重链基因SLA-2和轻链基因β2m连接,形成复合体基因链SLA-2-(G4S)3-β2m,然后将复合体基因链克隆入p2X表达质粒,导入受体菌TB1并进行表达。结果显示,利用SOE PCR技术成功得到了复合体基因链SLA-2-(G4S)3-β2m,并且克隆入p2X载体。SDS-PAGE检测表明,复合体基因导入宿主菌后获得了融合蛋白MBP-SLA-2-(G4S)3-β2m,大小为84.1 ku。试验结果为今后在体外进行相关基因重组表达提供参考。     

贵阳市奶牛小区奶牛繁殖性能调查

本文就贵阳市具有典型代表性的某奶牛小区奶牛的繁殖性能进行调查,调查结果表明：该奶牛养殖小区2007～2009年的平均年受胎率为83.0%,产犊间隔时间为427.00±3.36d,产后子宫复旧时间为39.94±1.72d;产后瘫痪、胎衣不下、子宫内膜炎及死胎的发病率均有逐年下降的趋势,但乳房炎和肢蹄病的发病率却呈上升的趋势。该奶牛养殖小区的胎衣不下、乳房炎、子宫内膜炎及肢蹄病的发病率仍较高,严重影响了该小区的经济效益。