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991.
SONG Zhi-ming WANG Min LIU Yong HAO Bao-shun NIU Hai-ming LIU Ding-hui YU Shu-jie ZHOU Bin WU Lin YU Xian-guan LING Ye-sheng PENG Pei ZHU Jie-ming CHEN Lin QIAN Xiao-xian 《园艺学报》2014,30(8):1345-1350
AIM:To explore the effect of hydrogen sulfide on the senescence of human umbilical vein endothelial cells (HUVECs) induced by high glucose. METHODS:Senescence model was established by treating HUVECs with 33 mmol/L glucose for 48 h. The parameters were detected to demonstrate the effect of hydrogen sulfide on senescence and the mechanism involved was also investigated. RESULTS:In the cells treated with high glucose, the proliferation was attenuated with a higher number of senescence-associated β-galactosidase (SA-β-Gal) positive cells, and plasminogen activator inhibitor 1 (PAI-1) protein expression, malondialdehyde (MDA) production and NF-κB p65 activity were increased significantly, but the expression of superoxide dismutase 1 (SOD1) was decreased. However, the cell number and SOD1 expression were increased, and the number of SA-β-Gal positive cells, PAI-1 protein expression, MDA production and the activity of NF-κB p65 were decreased after sodium hydrosulfide (100 and 200 μmol/L) treatment.CONCLUSION:Exo-genous hydrogen sulfide prevents HUVECs against high glucose-induced senescence by suppressing oxidative stress and NF-κB p65 activity. 相似文献
992.
HAO Feng BAI Xue-song JU Xiao-hong FANG Fang ZANG Yu-xuan ZHU Hang-fei XIANG Guo-yan ZHANG Yun-qiao YUAN Zhong-hai 《园艺学报》2014,30(9):1633-1639
AIM:To investigate the expression of transmembrane protein 16A(TMEM16A) in Fischer rat thyroid follicular epithelial (FRT) cells and its electrophysiologic properties. METHODS:The eukaryotic expression vector of pUB6/V5-TMEM16A was constructed and transfected into FRT cells by liposome-mediated transfection. In order to obtain the high efficiency of gene transfection and expression, the quantity and ratio of lipid/DNA complexes were optimized. The FRT cells stably expressing TMEM16A were gained by the selection with blasticidin and confirmed by the techniques of RT-PCR and immunofluorescence. The expression and location of TMEM16A in the FRT cells were observed under an inverted fluorescence microscope. TMEM16A protein was associated with calcium-dependent chloride current, as measured with halide-sensitive fluorescent protein and patch-clamp technique. RESULTS:The results of double digestion and sequencing indicated that TMEM16A was cloned into pUB6/V5. The results of RT-PCR and immunofluorescence confirmed that TMEM16A was expressed in the FRT cells after transfection with TMEM16A. The classical calcium-activated chloride channel currents were recorded in the FRT cells stably expressing TMEM16A by the technique of patch-clamp and halide-sensitive fluorescent protein YFP-H148Q/I152L. CONCLUSION:The protein expression of TMEM16A in the FRT cells was observed. TMEM16A is the molecular identity of calcium-activated chloride channels. 相似文献
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研究了在重金属镉胁迫条件下,大弹涂鱼(Boleophthalmuspectinirostris)肝脏黄嘌呤氧化酶活性的变化。重金属镉胁迫12h时0·5mg/LCd2 组XOD酶活性和对照组比较显著升高(P<0·05);胁迫24h时,大弹涂鱼0·05mg/LCd2 组肝脏的XOD酶活性和对照组比较极显著升高(P<0·01),而0·5mg/LCd2 组酶活性结果却降低;恢复3d时0·5mg/LCd2 又极显著升高(P<0·01)。在镉胁迫解除5d时,3个浓度组的酶活性均极显著降低(P<0·01),其中0·05mg/LCd2 组和0·5mg/LCd2 组的酶活性和对照组酶活性比较无显著差异(P>0·05),5mg/LCd2 组的酶活性极显著低于对照组的酶活性(P<0·01)。实验结果表明,大弹涂鱼肝脏黄嘌呤氧化酶活性对水体中镉污染敏感,有可能用做海洋环境污染的生物标记。 相似文献
998.
淤泥湖团头鲂与梁子湖团头鲂杂交子一代的性状研究 总被引:2,自引:0,他引:2
团头鲂(Megalobrama amblycephala Yih)是一种优良的淡水养殖鱼类,但在家养条件下连续传代会引起经济性状指标下降。本试验通过原种选优,以优配优的杂交组合,得出淤泥湖团头鲂与梁子湖团头鲂杂交子一代和回交子代。经饲养观察,证明杂交子一代和回交子代都有良好的生长优势,含肉率和粗蛋白含量相对增高,经济性状较亲本更好。血清蛋白电泳分析结果表明,杂交子代与亲本存在着质的差异。 相似文献
999.
为了探究黄瓜苗期生长发育规律和育苗方式,以华南型黄瓜‘沪杂7号’和荷短型黄瓜‘春秋王2号’为试材,用卡尺对植株高度、茎粗度、子叶大小、叶片大小进行测量,用天平称量地上部、根鲜质量,电子秤称量产量,利用LI-6400型光合仪对气体交换参数净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)与蒸腾速率(Tr)进行测量。结果表明,黄瓜植株高度、茎粗度增长均似线形,育苗后期生长速度更快,叶片长度、宽度增长速度前期较快,后期略慢;定植前大苗光合作用明显好于小苗,地上部、根鲜质量均显著高于小苗;华南型、荷短型黄瓜大苗定植,较小苗定植采收期均提前10 d以上,同时总产量明显增加。大苗定植对黄瓜提前上市及增加收入有明显效果。 相似文献
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Ultra high molecular weight polyethylene (UHMWPE) containing benzophenone (BP) is photocrosslinked under continuous UV irradiation. BP addition as low as 0.77 mol% per repeating unit of UHMWPE is enough to increase the gel fraction up to 99.8 %. The crosslinks are formed through the recombination of methine radicals generated from the hydrogen abstraction of UHMWPE by the excited BP. The heat of fusion of UHMWPE reduces substantially and the peak decomposition temperature increases as much as 15.8 oC due to the crosslinked network, coupled with slight decrease in crystallinity. The tensile strength, modulus and toughness of the crosslinked UHMWPE film are significantly improved with increasing crosslinking density. The wear rate of the UHMWPE also remarkably decreases from 10.2 mg/million cycles to 1.9 mg/million cycles, which can minimize the wear of total hip joint anthroplasty bearings. 相似文献