林火干扰对土壤理化性质的影响

通过实地调查与实验室测定,分析林火干扰对昆明近郊云南省森林自然中心华山松和柏木人工林林地土壤理化性质的影响。结果表明,火烧后,华山松林下土壤密度比未过火区增加11.48％,土壤自然含水量、饱和持水量、毛管持水量、总孔隙度以及毛管孔隙度分别下降32.38％、16.21％、18.71％、9.94％、22.44％;柏木林地土壤密度比未过火区增加10.38％,自然含水量、饱和持水量、毛管持水量、总孔隙度以及毛管孔隙度分别下降23.55％、10.77％、23.22％、5.38％、12.91％。过火区华山松林下土壤pH、有效磷含量比未过火区分别增加5.81％、130.25％,全氮、全磷、全钾、水解氮和速效钾含量分别下降53.30％、28.30％、22.60％、39.74％、25.32％;过火区柏木林下土壤pH、有效磷含量比未过火区分别增加12.33％、176.34％,全氮、全磷、全钾、水解氮和速效钾含量分别减少了31.28％、31.25％、67.09％、40.66％、51.21％。火烧后林地土壤更加板结,土壤养分含量降低,不利于土壤肥力的改善。     

不同固含量低分子酚醛树脂浸渍改性杉木板材性能的研究

为改善杉木的物理力学性能,采用真空-加压浸渍工艺,对速生杉木进行低分子酚醛树脂增强改性处理,分析不同树脂增重率对改性杉木性能的影响,以及树脂在木材长度和厚度方向上的分布特点。结果表明,杉木经过固含量为10％、20％、30％的酚醛树脂浸渍处理后,其尺寸稳定性随树脂固含量的增加逐渐提高,静曲强度由64.5 MPa提高至75.0 MPa,弹性模量和表面硬度分别提高了6.1％、27.5％、48.2％和29.8％、63.1％、73.8％,但冲击韧性随着树脂增重率的增加而逐渐降低;树脂在木材长度方向上分布均匀,在厚度方向上木材表面多于其内部。     

中国苹果气候区划方法研究进展

苹果气候区划可为气候资源高效利用、苹果种植区域的合理布局提供参考.从苹果气候区划指标选择方法及区划方法2个方面,综述了苹果气候区划的研究进展,并讨论了未来苹果区划的研究重点,可为开展苹果气候区划的指标及区划方法选择提供参考.目前的研究,主要采用统计学方法分析苹果品质指标和气象条件的关系筛选对苹果品质影响较大的因子作为区划指标,利用聚类分析或者模糊数学的方法对研究区域进行区划;应用GIS技术开展苹果精细化区划是未来的主要发展方向,按照苹果不同品种、熟性和苹果用途开展气候区划,是未来精细化区划的研究重点.     

中华鳖血液生物化学指标的研究

中华鳖(Ｔｒｉｏｎｙｘｓｉｎｅｎｓｉｓ)在我国广泛分布,其养殖从80年代发展至今,已在特种水产养殖中占有重要地位。但对其血液的生理和生物化学研究还不充分,主要集中在血细胞方面[1,2],未见有关生物化学参数的报道。血液生理和生化指标是物种的重要特征之一,是物质代谢特征的反应,在疾病诊断中也具有重要意义。本研究测定了在养殖条件下健康中华鳖血液的主要生物化学指标,旨在为了解其生理特点和养殖中的疾病防治提供依据,此外为研究鳖及鳖血的药用价值提供参考。1　材料和方法实验用鳖19只,均购自保定养鳖场。其中1998年12月取温室养殖…     

Long non-coding RNA and diabetes mellitus

Long non-coding RNAs (lncRNAs) are a group of RNAs, which are longer than 200 nucleotides without containing functional open reading frame and cannot encode protein. The study of lncRNA will help to understand the multi-level expression regulatory network of the body, and is expected to provide the basis of prediction, diagnosis and treatment of complex diseases. Although the functions and mechanism of lncRNA remain unclear, some studies indicate that lncRNA is involved in the development of diabetes mellitus, and those lncRNAs may be new diagnostic markers and therapeutic targets.     

Protective effect of emodin on lung injury induced by hepatic fibrosis in rats

AIM:To explore the protective effect of emodin on lung injury induced by hepatic fibrosis in rats. METHODS:The hepatic fibrosis rat model was established with multiple pathogenic factors (CCl 4, ethanol, high fat, high cholesterol and low choline) and treated with different doses (20 mg/kg and 40 mg/kg) of emodin for 4 weeks. The hepatic index was measured. The biochemical indexes, endotoxin, homocysteine, albumin, aspartate aminotransferase,alanine aminotransferase, total bilirubin, total cholesterol and triglyceride, and hepatic fibrosis indexes, hyaluronic acid, laminin, collagen IV and procollagen Ⅲ, were detected. The histopathological changes of the liver were observed. The pulmonary index was determined. The histopathological changes of the lungs were observed. The levels of tumor necrosis factor α (TNF-α), malondialdehyde (MDA), nitric oxide (NO) and peroxynitrite (ONOO-) in the lung tissues were analyzed. RESULTS:The rat hepatic fibrosis model was successfully established. In model group, lung edema and inflammation occurred, and the pulmonary index and the levels of TNF-α, MDA, NO and ONOO- in the lung tissues were increased significantly. In emodin treatment groups, the pulmonary indexes were lower than that in model group. The pathological injury of the lung tissues was alleviated. The levels of TNF-α, MDA, NO and ONOO- in the lung tissues were decreased. CONCLUSION:Emodin has a protective effect on lung injury induced by hepatic fibrosis in rats.     

柑橘新品种‘华红橘 1 号’

 ‘华红橘1 号’为浙江地方红橘品种‘满头红’的芽变,具有丰产性好、品质优良、风味浓 郁、化渣性好、酸甜适度等优良性状。在湖北荆门果实成熟期为11 月中下旬。     

Potassium antagonizes damage of coronary artery induced by high salt intake

AIM：To investigate the effect of potassium treatment on coronary arterial impairment induced by high salt intake. METHODS：Sprague-Dawley rats (4-week-old, n=10 in each group) received distilled water (NS), water containing 1.5% NaCl (HS), or 1.5% NaCl and 0.5% KCl (HS+HP) for 16 weeks. Systolic blood pressure (SBP) was determined by tail plethysmography every 2 weeks. After 16 weeks of treatment, vascular remodeling, superoxide production, malondialdehyde (MDA) content, and endothelial nitric oxide synthase (eNOS) and gp91 expression in the coronary arteries were detected. RESULTS：After 16 weeks of salt loading, the rats in HS group was divided into salt sensitive subgroup and salt resistance subgroup according to the tail-cuff blood pressure. In this experiment, the salt-sensitive rats were selected as HS group. In HS group, salt loading significantly increased SBP, serum MDA and gp91 expression, decreased serum NO and eNOS expression in the coronary arteries, and induced the coronary artery remodeling compared with NS group. In salt-loaded SD rats, 16-week potassium treatment abrogated the effects induced by salt loading. CONCLUSION：High salt may affect structural and functional changes in coronary arteries by activating oxidative stress. Potassium treatment antagonizes the effect of high salt intake.     

Effect of component II of broccoli polypeptide on glioma cell apoptosis

AIM：To explore the effect of component II of broccoli polypeptide on the apoptosis in glioma cells. METHODS：Human glioma SHG-44 cells were cultured and divided into control group and 3, 10, 30 and 100 mg/L component II of broccoli polypeptide groups. Cell viability was detected by MTT assay. The apoptotic rates were examined by Annexin V/PI staining. The morphological changes of the cells were observed under inverted microscope. The protein expression of Bax and Bcl-2 was detected by immunocytochemistry and Western blotting. The protein level of caspase-3 was also examined by Western blotting. RESULTS：Treatment with component II of broccoli polypeptide for 24 h, 48 h or 72 h induced significant inhibition of viability of SHG-44 cells in a time- and dose-dependent manner. The results of Annexin V/PI staining showed that the apoptotic rates were increased in treatment groups in a dose-dependent manner. The density of glioma cells was decreased after treated with increasing concentrations of the drug, and the apoptotic bodies were observed under inverted microscope at 72 h. The results of immunocytochemistry and Western blotting showed that the expression of Bax protein was increased but Bcl-2 protein expression was decreased, and the ratio of Bax/Bcl-2 was increased significantly compared with control group (P<0.05 or P<0.01). The level of caspase-3 protein was increased in 30 and 100 mg/L component II of broccoli polypeptide groups compared with control group (P<0.01). CONCLUSION：The component II of broccoli polypeptide increases the ratio of Bax/Bcl-2 and activates caspase-3 protein, thus inducing the apoptosis of glioma cells.     

L-Carnitine inhibits H2O2-mediated NFATc3 nuclear translocation

AIM:To explore the effect of L-carnitine on nuclear factor of activated T-cells,cytoplasmic 3 (NFATc3) in cardiomyocytes under H2O2 stimulation. METHODS:Primary cultured neonatal rat myocardial cells were stimulated by H2O2 at concentration of 200 μmol/L for 12 h to induce oxidative stress injury. In treatment group, L-carnitine and cyclosporin A (CsA), a specific inhibitor of calcineurin (CaN), were administered 30 min prior to H2O2 stimulation. After treatment, total, cytoplasmic and nuclear NFATc3 protein levels were determined by Western blotting. The method of immunofluoresence was used to evaluate the distribution of NFATc3. RESULTS:H2O2 treatment produced no effect on the expression of total NFATc3, but caused its translocation from the cytosolic to nuclear compartment, which was greatly blunted by L-carnitine pretreatment. CONCLUSION: L-carnitine antagonized oxidative stress injury via alleviating NFATc3 nuclear translocation.