稻田生态种植模式—一年四熟栽培技术探讨

随着耕地资源、人口、粮食、环境矛盾的不断演化,我国人多地少、钱粮争地的矛盾将日益尖锐,复种多熟是缓解矛盾的有效途径之一.     

福建省龙岩市创建现代国有林场初探

介绍龙岩市国有林场的分布情况、资源状况、经营情况、人员状况和存在问题,对创建现代国有林场的意义、作用和蓝图及创建中的科学规划、体制建设、经济政策、人才建设、制度建设和法律法规保障等问题进行了探讨.     

基于矿山储量动态监测的研究

从资源经济的角度，对矿山储量动态监测的重要性进行评价，讨论了该监测对建立我国矿山企业珍惜和合理利用资源的经济机制，建设资源节约型社会，具有的重要意义。为全国全面开展矿山储量动态监测提供科学借鉴范例。     

新升本科院校图书馆读者的借阅统计与分析——以河池学院为例

通过对读者的借阅数据和馆藏数据进行统计分析,探讨了高校读者的阅读特点,藏书结构对学生的适应性,并对本馆馆藏规划提出了自己的建议。     

Cloning of L-PGDS Gene and Its Expression Pattern in Different Tissues and Testis at Different Development Stages in Cattle-yak

The purpose of this study was to clone the prostaglandin D synthase (L-PGDS) gene and identify its expression pattern in various tissues and testis at different development stages in cattle-yak,in order to provide experimental basis for studying the mechanism of L-PGDS gene in testis development of cattle-yak.The total RNA of heart,liver,spleen,lung,kidney,large intestine,small intestine,gastric,brain and testis at different development stages (fetal,calve tuberty and old) of healthy cattle-yak were extracted by Trizol method.The L-PGDS gene sequence was amplified and cloned by RT-PCR,and analyzed by related biological software.The expression of L-PGDS gene mRNA in different tissues and testicular tissues at different development period of in cattle-yak were detected by Real-time quantitative PCR.The results showed that the length of L-PGDS gene was 624 bp,including the ORF of 576 bp,which encoded 191 amino acids.Homology alignment results showed that L-PGDS gene in cattle-yak had high homology with Bos taurus and Ovis aries,which were 99.28% and 94.00%,respectively.Phylogenetic tree results showed that the relation between cattle-yak and Bos taurus was the closest,followed by Ovis aries and Equus caballus.The L-PGDS protein was an unstable hydrophobic protein,the molecular weight was 21.229 ku,the molecular formula was C₉₅₃H₁₄₇₁N₂₅₁O₂₈₁S₉,the isoelectric point was 6.43,and the instability index was 47.25.The L-PGDS protein had no transmembrane region and no signal peptide.The secondary structure of L-PGDS protein contained alpha helix,random coil and extended chain,the rats of them were 25.65%,53.40% and 20.94%,respectively.L-PGDS gene was highly expressed in testis of cattle-yak,which was extremely significantly higher than that in other tissues (P<0.01).The expression of L-PGDS gene mRNA in testis of cattle-yak increased firstly and then decreased along with age,and with the highest expression in puberty stage,which was extremely significantly higher than that at other periods (P<0.01).This study provided basic data for further study on the mechanism of L-PGDS gene in the development of testis in cattle-yak.     

马铃薯晚疫病避病栽培技术及在迪庆州应用

马铃薯晚疫病是世界性的毁灭性病害,也是影响迪庆马铃薯产业发展的一个重要因素,如果不引起高度重视,采取有效防范措施,将会使迪庆马铃薯产业崩溃。而马铃薯晚疫病防治较为困难,必须采用综合防治的措施来控制或减轻发生危害,为提高预防及综合防治能力,迪庆州土肥站对马铃薯晚疫病避病栽培技术进行了研究。研究成果经在迪庆冬作马铃薯生产中应用,取得了良好的避病增产效果。     

柑桔慢衰线虫病在四川的发生危害研究

 对四川省36个柑桔产区324个柑桔园的调查结果表明,柑桔慢衰线虫病 (Tylenchulus semipenetrans)在四川普遍发生,病情趋势是川东部盆地区发生最重,盆周低、中山区次之,川西南干热河谷较轻.果园平均发病率为87.4%,病情Ι级园占35.6%,Ⅱ级占24.6%,Ⅲ级占20.4%,Ⅳ级占6.7%.每年引起的经济损失达2.3%～45%;对18种柑桔品种田间发病情况调查结果:酒饼勒未见发病,香橼、江南柑、曼桔发病较轻,枳对该病抗性表现不一致,其余均严重感病,香橼、江南柑、曼桔发病较轻,枳对该病抗性表现不一致,其余均严重感病;T.semipenetrans田间群体密度周年消长规律研究结果,土中侵染幼虫群体密度以春、秋季最大,夏季最小;根上侵染幼虫群体密度以8月最小,秋、冬最大;而雌成虫全年在根上的发生量均低,而且变动幅度不大.     

水红杆天麻及乌天麻有性杂交种与原品种间的比较研究

该试验利用水红杆天麻与乌天麻两种不同的优点，采用人工授粉的杂交方式，获得有性繁殖种子。在同等条件下，将有性繁殖杂交种与原品种进行比较试验。结果表明，有性杂交麻种在产量、成品率、折干率和抗病能力等方面均比原品种高。