Pilot Production of Hatchery-Reared Summer Flounder Paralichthys dentatus in a Marine Recirculating Aquaculture System: The Effects of Ration Level on Growth, Feed Conversion, and Survival

Pilot‐scale trials were conducted to evaluate growout performance of hatchery‐reared summer flounder fingerlings in a state‐of‐the‐art recirculating aquaculture system (RAS). The outdoor RAS consisted of four 4.57‐m dia × 0.69‐m deep (vol. =11.3 m³) covered, insulated tanks and associated water treatment components. Fingerlings (85.1 g mean initial weight) supplied by a commercial hatchery were stocked into two tanks at a density of 1,014 fish/tank (7.63 kg/m³). Fish were fed an extruded dry floating diet consisting of 50% protein and 12% lipid. The temperature was maintained between 20 C and 23 C and the salinity was 34 ppt. Under these conditions, growth, growth variation (CV_wt), feed utilization, and survival of fish fed to 100% and 82% of a satiation rate were compared. Due to clear changes in growth patterns during the study, data was analyzed in three phases. During phase 1 (d 1–d 196), fish showed rapid growth, reaching a mean weight of 288 g ± 105 and 316 g ± 102, with a CV_wt of 0.36 and 0.32 and FCR's of 1.38 and 1.36 in the subsatiation and satiation groups, respectively. During phase 2 (d 196–d 454), fish displayed slower growth reaching mean weights of 392 g ± 144 and 436 g ± 121, with a CV_wt of 0.37 and 0.28, and increasing FCR's of 3.45 and 3.12 in the subsatiation and satiation groups, respectively. During phase 3 (d 454–d 614), fish showed little growth reaching mean weights of 399 g ± 153 and 440 g ± 129, with a CV_wt of 0.38 and 0.29 in the subsatiation and satiation groups, respectively. Over the entire growout period (d 1–d 614), feed conversion ratios were 2.39 and 2.37 and survival was 75% and 81 % in the subsatiation and satiation treatments, respectively. The maximum biomass density reached during the study was 32.3 kg/m³. The satiation feed rate was superior to the 82% satiation rate, since it maximized growth rates, with no effect on FCR. The higher CV_wt in the subsatiation group suggests increased competition for a restricted ration led to a slower growth with more growth variation. The decrease in growth in phases 2 and 3 was probably related to a high percentage of slower growing male fish in the population and the onset of sexual maturity. This study demonstrated that under commercial scale conditions, summer flounder can be successfully grown to a marketable size in a recirculating aquaculture system. Based on these results, it is recommended that a farmer feed at a satiation rate to minimize growout time. More research is needed to maintain high growth rates through marketable sizes through all‐female production and/or inhibition of sexual maturity.     

Salinity Tolerance of Rice (Oryza sativa L.) with Reference to Endogenous and Exogenous Abscisic Acid

Experiments conducted m a phytotron on three rice varieties of different salinity tolerance revealed an increase in the content of endogenous abscisic acid (ABA) with increasing NaCl salinity in IR20 (semi salt-tolerant), but in Pokkali (salt-tolerant) and IR28 (salt-sensitive) the increase in ABA content was marginal. Under sahnity stress, in general, 5 weekly sprayings of ABA (10^-4 mol L^-1) decreased Na and K concentrations in the shoot to the extent of 29.5 % and 3.3 %, respectively. However, ABA application significantly improved the K/Na ratio as well as the chlorophyll fluorescence decrease ratio (Rfd, indicator for potential photosynthetic activity), the number of green leaves per plant and the shoot dry weight. The response of IR20 and IR28 to ABA application was significantly better than that of Pokkali. Increasing salinity caused marked nutrient imbalances, decreased Rfd values and shoot dry weight. The results are discussed in relation to possible mechanism of salinity tolerance.     

The problem of the pathomorphological diagnosis "death by drowning"--case histories

The paper describes pathomorphological findings in two dogs and one cat with the intention to clarify whether these animals died by drowning. The investigations are supplemented by experimental results of water instillation into the lung to induce signs of emphysema aquosum post mortem. Additionally, histopathology of the skin lesions due to water exposition after death are demonstrated by systematic investigations of four different localizations for 10 days. Critical evaluation of the results and literature references in human forensic pathology indicate that post mortem findings as described in this paper, especially those of the so called "drowning lung" can be attributed to death by drowning.