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91.
1. White blood cell responses of broilers, turkeys and ducks were examined at regular intervals after being subjected to various degrees of food restriction. 2. Restricted-fed broilers showed increases in heterophil and basophil numbers, together with a corresponding decrease in lymphocytes. The heterophil/lymphocyte ratio was raised. There were no differences between broiler strains. 3. After only one week of feeding restricted diets, heterophils were significantly raised in selected and unselected 2-week-old ducks. At 21 weeks of age, those ducks receiving 50% of food required to achieve their ad libitum-fed body weight had raised heterophils. 4. Ducks receiving food to achieve 25% of ad libitum-fed birds produced a marked basophilia, but no heterophilia. 5. After two weeks of food restriction, turkeys responded with significant heterophil/lymphocyte ratios following two degrees of restricted feeding. 6. It was concluded that in some poultry, a heterophilia may be the response to mild to moderate stress but a basophilia may result after severely stressing birds. 相似文献
92.
Three-week-old weaned and colostrum-deprived neonatal (less than 1 day old) pigs were inoculated to determine the pathogenicity of 2 enterotoxigenic Escherichia coli isolates that do not express K88, K99, F41, or 987P adhesins (strains 2134 and 2171). Strains 2134 and 2171 were isolated from pigs that had diarrhea after weaning attributable to enterotoxigenic E coli infection. We found that both strains of E coli adhered in the ileum and caused diarrhea in pigs of both age groups. In control experiments, adherent bacteria were not seen in the ileum of pigs less than 1 day old or 3 weeks old that were noninoculated or inoculated with a nonpathogenic strain of E coli. These control pigs did not develop diarrhea. Antisera raised against strains 2134 and 2171 and absorbed with the autologous strain, grown at 18 C, were used for bacterial-agglutination and colony-immunoblot assays. Both absorbed antisera reacted with strains 2134 and 2171, but not with strains that express K99, F41, or 987P adhesins. A cross-reaction was observed with 2 wild-type K88 strains, but not with a K12 strain that expresses K88 pili. Indirect immunofluorescence with these absorbed antisera revealed adherent bacteria in frozen sections of ileum from pigs infected with either strain. We concluded that these strains are pathogenic and express a common surface antigen that may be a novel adhesin in E coli strains that cause diarrhea in weaned pigs. 相似文献
93.
用免疫荧光、酶标技术检测赭曲霉毒素A的研究 总被引:1,自引:0,他引:1
该项研究采用本课题组提纯的赭曲霉毒素A(Ochratoxin A·简称OA)和研制的兔抗OA抗体等,进行间接荧光法和ELISA间接法检测OA的试验。通过对不同浓度的OA纯品和含毒饲料浸提液及中毒死亡鸡内脏的间接荧光染色,均能检出分布均匀、大小有别的黄绿色荧光亮点;用OA为被检抗原和兔抗OA抗体与羊抗兔IgG-HRP进行ELISA间接法试验,对含不同浓度OA的试验孔和对照孔的检测,结果均正确稳定。研究结果证明,以上两种检测方法检测OA均具有灵敏、特异、快速等优点。 相似文献
94.
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96.
本言语报道昆明小哨和宜良良竹山两地区两种植被类型和两种成土母质发育的土壤上,干巴菌只能在弱酸性的沉积岩类(石灰岩或砂页岩)或河流沉积物类成土母质发育形成的山原红壤的针叶林型的云南松或阔叶林型的麻栎林下生长发育。而其它成土母质形成的土壤中云南松林或麻栎林下,不会长出干巴菌。(2)干巴菌根际土壤必须具有良好的理化性质--土壤疏松多孔、透水透气和土温传递优良;PH值在4.5-5.5区间内;土质富含钙和铁 相似文献
97.
576只190日龄蛋用父母代海兰种鸡随机分为3组,分别在常规料供钙、傍晚额外加喂供钙和早晨及下午各供钙一次的三种方式下饲养8周。结果表明:早晨和下午各一次的供钙方式提高了蛋鸡的生产性能,较常规料供钙方式和傍晚额外加喂钙方式蛋重分别提高3.503%(P<0.01)和3.658%(P<0.01),产蛋量提高3.185%(P<0.01)和3.992%(P<0.01),饲料报酬提高4.485%(P<0.01)和5.298%(P<0.01)。大幅度降低了不合格蛋比例,和常规料供钙方式差异显著(P<0.05),额外加喂供钙方式使鸡产沙壳蛋的比例上升,早晨和下午供钙方式显著改善了鸡蛋的蛋壳品质。 相似文献
98.
将构建的pBST2~6工程菌质粒用限制性内切酶BamHI/BglⅡ酶切,经琼脂糖凝胶电泳和电洗脱,回收147bp的目的ST1基因。随之将该基因分别重组到能有效表达K99菌毛抗原和LacZ酶的pGK99之K99基因BglⅡ位点和pUC18的BamHI位点中。通过ST1基因探针菌落原位杂交、特定酶切分析及DNA序列分析,筛选并鉴定出了理想重组子,从而构建出了能分别表达ST1融合基因产物的工程菌株pSK219和pXST1。 相似文献
99.
A technique is described for biopsy of the bovine udder, employing sedation and local anaesthesia. Tissue samples of approximately 5 g were obtained by electrocautery from two quarters of the udder of a cow laterally recumbent. Care was taken to ensure complete haemostasis which was achieved by electrocoagulation and ligation. Postoperative recovery was rapid, and loss of yield was no greater in biopsied glands than in control glands of the same cow. Yield from all quarters returned to preoperative levels within 48 h. 相似文献
100.
The pyrimidine analog 5-bromodeoxyuridine (BUdR) competes with thymidine for incorporation into DNA. Substitution of BUdR for thymidine does not significantly affect cell viability but does block cell differentiation in many different lineages. BUdR substitution in a mouse myoblast line blocked myogenic differentiation and extinguished the expression of the myogenic determination gene MyoD1. Forced expression of MyoD1 from a transfected expression vector in a BUdR-substituted myoblast overcame the block to differentiation imposed by BUdR. Activation of BUdR-substituted muscle structural genes and apparently normal differentiation were observed in transfected myoblasts. This shows that BUdR blocks myogenesis at the level of a myogenic regulatory gene, possibly MyoD1, not by directly inhibiting the activation of muscle structural genes. It is consistent with the idea that BUdR selectively blocks a class of regulatory genes, each member of which is important for the development of a different cell lineage. 相似文献