Immunochemical study of canine intestinal, hepatic, and osseous alkaline phosphatases.

Partially purified alkaline phosphatase (ALP) from canine intestine, liver, and bone were injected into rabbits to elicit anti-canine intestinal, hepatic, and osseous ALP antibodies, respectively. The antibody formed a soluble enzyme-antienzyme complex when directly interacted with the ALP antigen. In order to form an insoluble complex, it was then necessary to interact the initial soluble complex with the goat anti-rabbit gamma-globulin antibody in the 2nd step. Antiintestinal ALP antibody was highly specific and did not cross react with canine hepatic, osseous, splenic, and renal ALP. Antiliver and antibone ALP antibodies, on the other hand, did cross react with hepatic, osseous, splenic, and renal ALP, but not with the intestinal ALP.     

INTERACTION BETWEEN INFECTIOUS BURSAL DISEASE VIRUS AND NEWCASTLE DISEASE VIRUS IN CHICKENS

The Australian strain of infectious bursal disease virus (IBDV), 002/73, affected the response of chickens to Newcastle disease virus (NDV). The titre of serum antibodies to NDV in chickens infected with IBDV was significantly lower than that of birds infected with NDV alone. It also appeared that IBDV affected NDV excretion from chickens as NDV was more frequently isolated from chickens infected with IBDV, IBDV infection did not alter the pathogenicity of NDV in chickens. This Australian strain of IBDV therefore appeared to be immunodepressive in one-day-old chickens.     

Salmonella serotypes encountered in animal feed additives in Lebanon.

Animal feed-additive samples (n = 300) were examined for the presence of salmonellae, using the selenite-F broth-enrichment method followed by subculturing on Salmonella-Shigella and brilliant green agar with sulfadiazine selective agar plates. Samples consisted of a variety of feed additives: 119 bone meal samples, 77 meat meal samples, 40 fish meal samples, and 64 miscellaneous meal samples. Results of examination found 49 (41.2%) of the bone meal samples, 6 (7.8%) of the meat meal samples and 2 (5%) of the fish meal samples contained salmonellae. Of 57 isolates representing 24 serotypes, 4 most frequently isolated serotypes were Salmonella meleagridis (35.1%), Salmonella tennessee (7%), Salmonella chester (5.2%), and Salmonella senftenberg (5.2%). This study shows a high Salmonella-contamination rate of bone meal compared with meat meal and fish meal samples. Of 12 known positive bone meal samples that were examined, 100% of 25-g samples, compared with 70% to 100% of 2.5-g samples and 30% to 90% of 0.25-g samples and 30% to 90% of 0.25-g samples, were positive for salmonellae.     

Differentiation of influenza virus antigens using enzyme immunoassay]

Reported is a microprocess of enzyme immune assay in which slipformed air pockets of polyvinylchloride (PVC), as used in the pharmaceutical industry, are used as carriers of antigens or antibody. Two methods, the anti-globulin and the double-antibody methods, are based on antibody which had been coupled with alkaline phosphatase. Tests in which various sub-types of influenza virus were used have shown the double-antibody method to be a sensitive technique which can be successfully used in the differentiation of envelope antigens.     

Microbiology of normal and seborrheic canine skin.

Quantitative and qualitative bacterial assays were performed on the skin of 15 normal and 32 seborrheic dogs. Nonionic detergent scrubs were made on areas demarcated by glass sidearm cylinders. Quantitative analysis was accomplished by the serial dilution technique, and the bacteria were identified by individual and colonial morphology and by enzyme production. Areas measured on control dogs had a markedly lower total bacterial count than similar areas measured on seborrheic animals. Control dogs had a flora consisting primarily of coagulase-negative cocci, whereas seborrheic dogs usually had a cutaneous flora composed primarily of Stahylococcus aureus, coagulase-positive.     

Isolation and identification of a bovine adenovirus type 3 with an adenovirus-associated virus.

A bovine adenovirus with agglutinating activity was isolated from feedlot calves and classified as serotype 3. The agglutinating activity was shown to be the property of an adenovirus-associated virus (AAV). The AAV was isolated from the bovine adenovirus by isopycnic centrifugation in CsCl; the AAV had a density of 1.4 g/cm2. This AAV is serologically related to bovine AAV-TR-15, but is distinct from bovine parvovirus-1 and primate AAV types 1 to 4, using counterimmunoelectrophoresis and hemagglutination-inhibition.