Use of a three-drill-tract technique for arthrodesis of the distal tarsal joints in horses with distal tarsal osteoarthritis: 54 cases (1990-1999)

OBJECTIVE: To assess the long-term clinical outcome of horses with distal tarsal osteoarthritis (OA) in which a 3-drill-tract technique was used to induce arthrodesis of the affected joints, identify any preoperative or operative factors associated with outcome, and describe any complications associated with the technique. DESIGN: Retrospective study. ANIMALS: 54 horses. PROCEDURE: Medical records were reviewed for information on signalment, use, history, physical and lameness examination findings, surgical technique, and postoperative care. Radiographs were examined, and severity of OA was graded. Follow-up information was obtained through telephone interviews with owners at least 13 months after the procedure. RESULTS: 32 (59%) horses had a successful outcome, 6 (11%) improved but were not sound after surgery, and 16 (30%) did not improve following surgery. Outcome was negatively associated with the previous use of intra-articular injections. Few postoperative complications were evident. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that distal tarsal OA in horses can be successfully treated by means of distal tarsal arthrodesis with a 3-drill-tract technique. Horses with advanced distal tarsal OA are likely to have poorer outcomes, and the procedure will likely be of minimal benefit in horses with concomitant causes of hind limb lameness prior to surgery and in horses with preexisting proximal intertarsal joint disease.     

Determination of skin threshold concentration of an aqueous house dust mite allergen in normal dogs

Fifty-six healthy dogs with no known history of atopic disease to indoor allergens were skin tested with 6 different dilutions of an aqueous house dust mite extract. A concentration of 31.25 PNU/ml was found to be the maximum, nonirritating concentration. Thirty-two percent of the research dogs used had to be excluded because they failed to show reactions to any test dilution.     

Metastatic immune infiltrates correlate with those of the primary tumour in canine osteosarcoma

Our lack of understanding of the immune microenvironment in canine osteosarcoma (cOSA) has limited the identification of potential immunotherapeutic targets. In particular, our ability to utilize readily available tissue from a dog's primary tumour to predict the type and extent of immune response in their pulmonary metastatic lesions is unknown. We, therefore, collected 21 matched pairs of primary tumours and pulmonary metastatic lesions from dogs with OSA and performed immunohistochemistry to quantify T‐lymphocyte (CD3), FOXP3+ cell, B‐lymphocyte (Pax‐5), and CD204+ macrophage infiltration. We found that T‐lymphocytes and FOXP3+ infiltrates in primary tumours positively correlated with that of metastatic lesions (ρ = 0.512, P = 0.038 and ρ = 0.698, P = 0.007, respectively), while a strong trend existed for CD204+ infiltrates (ρ = 0.404, P = 0.087). We also observed T‐ and B‐lymphocytes, and CD204+ macrophages to be significantly higher in a dog's pulmonary metastasis compared to their primary tumour (P = 0.018, P = 0.018, P = 0.016, respectively), while FOXP3+ cells were only significantly higher in metastases when all primary tumour and metastasis lesions were compared without pairing (P = 0.036). Together, these findings suggest that the metastatic immune microenvironment may be influenced by that of the primary cOSA, and that primary tumour immune biomarkers could potentially be applied to predict immunotherapeutic responses in gross metastatic disease. We, therefore, provide a rationale for the treatment of cOSA pulmonary metastases with immunotherapeutics that enhance the anti‐tumour activity of these immune cells, particularly in dogs with moderate to high immune cell infiltration in their primary tumours.     

New ELISA test for detection of bovine leukemia virus infections in cattle, using bacterially synthesized p24

A new ELISA test is described for the detection of antibodies to bovine leukemia virus protein p24. This test employs a bacterially synthesized p24 antigen which represents a hybrid protein consisting of beta-galactosidase and about 70% of the mature viral p24. The antigen preparation was enriched from Escherichia coli cells to 95% purity and was used for the detection of antibodies in cattle. In a selected set of 100 positive field sera, 97 could be verified by the new test.     

Physiological responses of young thoroughbred horses to intermittent high-intensity treadmill training

Background

Training of young Thoroughbred horses must balance development of cardiopulmonary function and aerobic capacity with loading of the musculoskeletal system that can potentially cause structural damage and/or lameness. High-speed equine treadmills are sometimes used to supplement exercise on a track in the training of young Thoroughbreds because the horse can run at high speeds but without the added weight of a rider. We tested the hypothesis that intermittent high-intensity exercise on a treadmill of young Thoroughbred horses entering training can enhance development of aerobic capacity (Vo₂max) and running performance more than conventional training under saddle, and do so without causing lameness.

Results

Twelve yearling Thoroughbreds trained for 8 months with conventional riding (C) only, conventional riding plus a short (2 month, S) interval of once-per-week high-intensity treadmill exercise, or a long (8 month, L) interval of once-per-week high-intensity treadmill exercise. Three treadmill exercise tests evaluated Vo₂max, oxygen transport and running performance variables in June of the yearling year (only for L), October of the yearling year and April of the 2-year-old year. No horses experienced lameness during the study. Aerobic capacity increased in all groups after training. In both October and April, Vo₂max in L was higher than in C, but did not differ between L and S or S and C. Running speeds eliciting Vo₂max also increased in all groups after training, with S (809 ± 3 m/s) and L (804 ± 9 m/s) higher than C (764 ± 27 m/s). Maximum heart rate decreased for all groups after training. Hematocrit and hemoglobin concentration increased for L throughout training.

Conclusions

Young Thoroughbred horses can increase aerobic capacity and running performance more than by strictly using track training under saddle with the addition of intermittent high-intensity treadmill exercise, and they can do so without experiencing lameness. This finding suggests that young racehorses might be able to achieve higher aerobic fitness during training without subjecting their musculoskeletal systems to increased loading and risk of developing lameness. The findings of this preliminary study do not indicate a specific protocol to best achieve this goal.     

Removal of Iron and Manganese from Water with a High Organic Carbon Loading. Part I: The Effect of Various Coagulants

Iron and manganese removal from water is considered a routine operation in most potable water treatment plants. However, when there is dissolved organic carbon (DOC) present in the water source, it can significantly influence the success of iron and manganese removal. This investigation focused on the use of two different coagulants, alum and ferrichloride, used in conjunction with hydrogen peroxide as an oxidant, to reduce dissolved iron and manganese in water with a high DOC loading. The two coagulants behaved in a totally different manner during the treatment process, with alum being successful in reducing the iron content in the treated water, and ferrichloride yielding a good removal of the dissolved manganese present. Since these results were only possible in cases where hydrogen peroxide concentrations were also present, it was concluded that treatment with a single reagent would not yield the desired removal of contaminants from this specific water. Both treatment options significantly reduced the DOC levels in the water to below the desired concentration after treatment.     

A class of potent antimalarials and their specific accumulation in infected erythrocytes

During asexual development within erythrocytes, malaria parasites synthesize considerable amounts of membrane. This activity provides an attractive target for chemotherapy because it is absent from mature erythrocytes. We found that compounds that inhibit phosphatidylcholine biosynthesis de novo from choline were potent antimalarial drugs. The lead compound, G25, potently inhibited in vitro growth of the human malaria parasites Plasmodium falciparum and P. vivax and was 1000-fold less toxic to mammalian cell lines. A radioactive derivative specifically accumulated in infected erythrocytes to levels several hundredfold higher than in the surrounding medium, and very low dose G25 therapy completely cured monkeys infected with P. falciparum and P. cynomolgi.     

Blood lead levels of wild Steller's eiders (Polysticta stelleri) and black scoters (Melanitta nigra) in Alaska using a portable blood lead analyzer.

Sea duck populations are declining in Alaska. The reasons for the decline are not known; environmental lead exposure is one suspected cause. Thirty wild Steller's eider ducks (Polysticta stelleri) and 40 wild black scoter ducks (Melanitta nigra) were tested for blood lead levels using a portable blood lead analyzer (LeadCare; ESA, Inc., Chelmsford, Massachusetts 01824, USA). Sixty-seven and one-tenth percent of the sea ducks had undetectable blood lead levels, 30.0% had values indicating normal or background lead exposure, and 2.9% had values indicating lead exposure. None of the birds had values indicating lead toxicity, and no birds demonstrated clinical signs of toxicity. Birds in areas with higher human population density had higher blood lead levels than those in less densely populated areas. This is the first time a portable blood lead analyzer has been utilized with sea ducks in a field setting. Because it provides immediate results, it is valuable as a screening tool for investigators carrying out surgical procedures on birds in the field as well as establishing baseline blood lead data on sea ducks. Lead exposure does occur in wild sea ducks, and the study indicates that additional research is needed in order to determine the role environmental lead plays in declining sea duck populations.     

Chemisch-mineralogische und mikromorphologische untersuchung einer eutrophen (Ramann-) braunerde des Budapester Hügellandes

In spite of the fact that Ramann's concept of Braunerde was extensively used throughout the world, some pedogenic processes and soil properties which are important for the formation and existence of Brown Forest Soils (eutrophic brown earths) are still not well known. Hence, a soil was investigated which was classified as a “Braunerde” by Ramann in 1909. The main question was, whether there might be soil constituents such as inorganic amorphous substances (allophanes) which are able to stabilize the brown-earth fabric, or to inhibit clay migration. From chemical data as well as from the magnitude of the pH-dependent CEC, it was deduced that there are no appreciable amounts of allophanes within the soil. The fabric, however, seems to be stabilized by colloid-chemical flocculation of the clay particles caused by large amounts of carbonates and silicates deposited with the parent material (loess). The decalcified solum is still rich in silicates, especially sand and coarse-silt-sized micas and feldspars (60–200 and 20–60 μm fractions, respectively). Since - in comparison - loesses and loess-derived soils of the Central German mountain region contain less silicates, mainly in fine and medium silt fractions (2–6 and 6–20 μm diameter, respectively), the coarser-grained silicates were assumed to be important for maintaining the Ca²⁺ and Mg²⁺ saturation of the soil by continuous weathering and thus stabilizing the brown-earth fabric.Although the soil is saturated predominantly with Ca²⁺ and Mg²⁺ ions, clay migration proceeds within the upper horizons. This was shown by calculation of the amounts of clay formed by breakdown of micas originally present in the parent material (“clay formation balance”), as well as by micromorphological studies. Furthermore, micromorphological studies and x-ray diffraction data gave some evidence for the migration of preferably finest grained montmorillonitic clays (smectites) penetrated by organic substances (humus). This kind of “selective clay migration” was assumed to be caused by high Ca²⁺ (or Mg²⁺) concentrations in soil solution required for flocculation of humus-penetrated (humus-coated) smectites. From the occurrence of these “humus-smectites” the possibility was assumed that the soil studied has been developed from a former Chernozem type.Quantitatively, clay migration does not reach the amounts of clay formation (breakdown of micas) accompanied by precipitation of iron oxides on mineral surfaces (“Verbraunung”). Thus, the soil profile visually and macromorphologically clearly exhibits the features of brown earths. On the other hand, however, some clay migration was observed. Hence, the soil was classified as a “brown earth with some clay migration” (“schwach durchschlämmte Braunerde”).Judging from the results of the “clay formation balance” an appreciable pedogenic (autigenic) clay formation from weathering products of feldspars was excluded for the soil studied.