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73.
Limited sampling pharmacokinetics of subcutaneous ondansetron in healthy geriatric cats,cats with chronic kidney disease,and cats with liver disease 下载免费PDF全文
R. L. Fitzpatrick L. A. Wittenburg R. J. Hansen D. L. Gustafson J. M. Quimby 《Journal of veterinary pharmacology and therapeutics》2016,39(4):350-355
Ondansetron, a 5‐HT3 receptor antagonist, is an effective anti‐emetic in cats. The purpose of this study was to compare pharmacokinetics of subcutaneous (SQ) ondansetron in healthy geriatric cats to cats with chronic kidney disease (CKD) or liver disease using a limited sampling strategy. 60 cats participated; 20 per group. Blood was drawn 30 and 120 min following one 2 mg ( mean 0.49 mg/kg , range 0.27–1.05 mg/kg ) SQ dose of ondansetron. Ondansetron concentrations were measured by liquid chromatography coupled to tandem mass spectrometry. Drug exposure represented as area under the curve (AUC) was predicted using a limited sampling approach based on multiple linear regression analysis from previous full sampling studies, and clearance (CL/F) estimated using noncompartmental methods. Kruskal–Wallis anova was used to compare parameters between groups. Mean AUC (ng/mL·h) of subcutaneous ondansetron was 301.4 (geriatric), 415.2 (CKD), and 587.0 (liver). CL/F (L/h/kg) of SQ ondansetron was 1.157 (geriatric), 0.967 (CKD), and 0.795 (liver). AUC was significantly higher in liver and CKD cats when compared to geriatric cats (P < 0.05). CL/F in liver cats was significantly decreased (P < 0.05) compared to geriatric cats. In age‐matched subset analysis, AUC and CL/F in liver cats remained significantly different from geriatric cats. 相似文献
74.
T. Chikmawati M. Miftahudin B. Skovmand J. Perry Gustafson 《Genetic Resources and Crop Evolution》2012,59(8):1743-1752
Amplified fragment length polymorphism markers were evaluated to determine the genetic diversity and relationships among cultivated and weedy ryes (Secale cereale L.) using a large global set of accessions. On the basis of 395 polymorphic bands resulted from nine PstI-MseI primer combinations, cultivated rye exhibited higher average genetic diversity (Ht?=?0.34) than that of the weedy rye (Ht?=?0.27), however, it had lower genetic differentiation (Fst?=?0.16). The average genetic diversity of cultivated rye varied from region to region ranging from 0.21 to 0.31. As expected, all cultivated accessions clustered together both in dendrogram and principal coordinate diagram indicating common breeding program selection criteria based on similar value-added agronomic characteristics. A clustering of cultivated rye accessions into groups based strictly on geographical origin was not found. The relationships among cultivated, weedy and wild ryes were discussed. 相似文献
75.
Gunnela M Gustafson Ingemar Olsson 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(1):10-19
The partitioning of nutrients and trace elements from feeds, drinking water and mineral supplements on growth, faeces and urine of growing cattle was studied with eight steers in a balanced Latin square design with two replicates, each comprising four treatments (diet compositions), four periods and four animals. The treatments were four rations with a silage proportion of 15, 30, 60 and 100% of the dry matter. Records were taken of the individual intake of silage, concentrates, mineral supplements and water, and excretions by collecting all urine and faeces. All feeds, urine, faeces and water were analysed for their content of Al, Ca, Cd, Cl, Co, Cu, Fe, K, Mg, Mn, Mo, N, Na, Ni, P, S, Se, and Zn. Elements that were mainly excreted in faeces (Ca, Cd, Cu, Fe, P, and Zn) tended to have a constant uptake in amount. As there is a fairly good correlation between DM consumption and faeces production, it is possible to estimate the amount of these elements excreted at farm level by analysing a faeces sample. Elements that are excreted in significant amounts in both faeces and urine (Cl, K, Mg, Mo, N, Na, S, and Se) tended to have a constant uptake over treatments as a fraction of the feed content. It is more difficult to estimate the amounts of these elements at farm level because it is more difficult to predict urine production than faeces production. 相似文献
76.
CJ MORROW † IG BELL† SB WALKER§ PF MARKHAM† BH THORP¶ KG WHITHEAR† 《Australian veterinary journal》1990,67(4):121-124
This report describes the first isolations of Mycoplasma synoviae from the synovial sheaths and joints of commercial chickens affected with synovitis in Australia. Over 4 years 3 separate outbreaks were investigated in which up to 20% of birds exhibited clinical signs of poor growth and "hot foot" syndrome (swollen inflamed footpads). Once an outbreak occurred, chronic infection of the farm usually ensued. Grossly the hocks and footpads were swollen by a purulent exudate and associated inflammatory changes with histological features of a severe acute synovitis. Seroconversion of the flocks occurred at the time of the development of lesions. M. synoviae specific antibodies were demonstrated by ELISA in the joint fluid of affected birds. It is concluded that the cases described are similar to avian infectious synovitis syndrome caused by M. synoviae previously described overseas. 相似文献
77.
Addition of Colchicine to Wheat Anther Culture Media to Increase Doubled Haploid Plant Production 总被引:3,自引:0,他引:3
W. Navarro-Alvarez P.S. Baenziger K.M. Eskridge M. Hugo V.D. Gustafson 《Plant Breeding》1994,112(3):192-198
Chromosome doubling is critical for obtaining doubled-haploid plants from wheat (Triticum aestivum L.) anther culture. The most common doubling method applies colchicine to the plant. However, colchicine is phytotoxic and can induce a high frequency of plant death. In this experiment, anthers from two wheat genotypes (“Pavon 76” and ‘Centurk’) were placed on nine embryoid initiation media having three sugar sources (maltose, sucrose, and maltose + glucose) with three colchicine concentrations (0.0, 0.1, and 0.2 g · l-1). Wheat starch was used as a gelling agent. After three days, the anthers were washed and moved to fresh media without colchicine. Increasing the colchicine concentration decreased the number of embryoids produced from 77.4 embryoids/100 anthers to 29.9 embryoids/100 anthers, but did not significantly affect the frequency of plant regeneration (0.49 green plants/embryoid to 0.40 green plants/embryoid), and increased the frequency of doubled-haploid plants (19.0 doubled-haploid plants/100 green plants to 72.3 doubled-haploid plants/100 green plants). Considering the total number of doubled-haploid plants produced, low levels of colchicine added to the initiation media were very effective. 相似文献
78.
The influence of telomeric heterochromatin on early embryo and endosperm development and the production of aberrant endosperm nuclei were investigated using eight triticale (×Tritocmecale Wittmack) lines homozygous for the presence or absence ol blocks of telomeric heterochromatin from rye chromosome arms 1RL, 2RS, (SRS, and 7RL. The loss of heterochromatin from two chromosomes (6R, 7R) produced a significantly lower percentage of aberrant endosperm nuclei than did the loss from one chromosome (6R). Deletion of a third block had a significant additional effect in only one of four lines. The differences between lines for rales of embryo and endosperm development were significant even within a karyotypc, presumably because of genetic variation. The onset of cellularization of the endosperm tended to obscure the detection of aberrancy, and since it evidently occurred at different times in different lines, may have been largely responsible for the apparent reduction in aberrant nuclei in lines with less heterochromatin. 相似文献
79.
H. Guedes-Pinto J. Lima-Brito C. Ribeiro-Carvalho J. P. Gustafson C. O. Qualset 《Plant Breeding》2001,120(1):27-31
Limited genetic knowledge is available regarding crossability between hexaploid triticale (2n= 6x= 42, 21″, AABBRR, amphiploid Triticum turgidum L.‐Secale cereale L.) and rye (2n= 14, 7″, RR). Our objectives were to determine (1) the crossability between triticales and rye and (2) the inheritance of crossability between F2 progeny from intertriticale crosses and rye. First, ‘8F/Corgo’, a hexaploid triticale, was crossed as a female with two landrace ryes, ‘Gimonde’ and, ‘Vila Pouca’ and two derived north European cultivars, ‘Pluto’ and ‘Breno’. These crosses produced 21.7, 20.9, 5.9, and 5.6%, seed‐set or crossability, respectively, showing that the landrace ryes produced higher seed‐set than the cultivars. Second, ‘Gimonde’ rye was crossed as a male with four triticales for 3 years. The control cross, ‘Chinese Spring’ wheat × rye, produced 80‐90% seed‐set. Of the four triticales, ‘Beagle’ produced 35.7‐56.8% seed‐set. The other three triticales produced less than 20% seed‐set, showing that the triticales differ in crossability with ‘Gimonde’ rye. Third, six FiS from intertriticale crosses (‘8F/Corgo’בBeagle’, ‘Beagle’בCachirulo’, ‘Lasko’בBeagle’, ‘8F/Corgo’בCachirulo’, ‘Lasko’בCachirulo’, ‘Lasko’ב8F/Corgo’) were crossed to ‘Gimonde’ rye. Results indicated that lower crossability trait was partially dominant in the two F1S from crosses involving ‘Beagle’(high crossability) with‘8F/Corgo’ and ‘Cachirulo’(low crossability) and completely dominant in the ‘Beagle’בLasko’ cross, as it happens in wheat. Fourth, segregants in four F2 populations (‘Lasko’בBeagle’, ‘8F/Corgo’בBeagle’, ‘Lasko’ב8F/Corgo’, and‘8F/Corgo’בCachirulo’) were crossed with rye. Segregation for crossability was observed, although distinct segregation classes were blurred by environmental and perhaps other factors, such as self‐incompatibility alleles in rye. Segregation patterns showed that ‘Beagle’, with high crossability to rye, carries either Kr1 or Kr2. The three triticales with low crossability with rye were most likely homozygous for Kr1 and Kr2. Therefore, it is likely that the Kr loci from A and B genomes acting in wheat also play a role in triticale × rye crosses. 相似文献
80.
Peridotite xenoliths from the Cascade arc in the United States and in the Japan arc have neodymium and osmium isotopic compositions that are consistent with addition of 5 to 15 percent of subducted material to the present-day depleted mantle. These observations suggest that osmium can be partitioned into oxidized and chlorine-rich slab-derived fluids or melts. These results place new constraints on the behavior of osmium (and possibly other platinum group elements) during subduction of oceanic crust by showing that osmium can be transported into the mantle wedge. 相似文献