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Colletotrichum lagenarium is the casual agent of anthracnose disease of melons. Light and scanning electron microscopy were used to observe the infection process of C. lagenarium on the leaves of two melon cultivars differing in susceptibility. On both cultivars conidia began germinating 12 h after inoculation (hai), forming appressoria directly or at the tips of germ-tubes. By 48 hai appressoria had melanised and direct penetration of host tissue had begun. On the susceptible cultivar, infection vesicles formed within 72 hai and developed thick, knotted primary hyphae within epidermal cells. By 96 hai C. lagenarium produced highly branched secondary hyphae that invaded underlying mesophyll cells. After 96 hai, light brown lesions appeared on the leaves, coincident with cell necrosis and invasion by secondary hyphae. While appressoria formed more quickly on the resistant cultivar, fewer germinated to develop biotrophic primary or invasive necrotrophic secondary hyphae than on the susceptible cultivar. These results confirm that C. lagenarium is a hemibiotrophic pathogen, and that resistance in melons restricts colonisation by inhibiting the development of necrotrophic secondary hyphae. 相似文献
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Although not without its critics, considerable recognition has been given to the climate cooling benefits provided by storing carbon from biomass in various storage pools. However, it has recently been found that depending on the storage pool/period and source of biomass, the associated climate impacts may be a burden or a benefit. It is important that carbon accounting schemes and life cycle assessment practitioners take these carbon/CO2 flux dynamics and the climate impacts that they create into consideration. In this work we illustrate these climate impacts with a Norwegian case study using a material flow analysis of the biogenic carbon in harvested wood products derived from a 2006 harvest year. We illustrate the dynamic carbon balance over time and show how the climate impacts can diverge greatly between two well-known climate impact metrics: global warming potential (GWP) and global temperature potential (GTP). We also show how these climate impacts can be attributed to contributing parties with an example of a glue laminated beam value chain which is stored in a long-lived building. We discuss the associated attribution issues that will inevitably arise and we offer recommendations on how best to minimize them. 相似文献
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