鸡痘病毒282E_4株TK基因重组载体质粒的构建

应用已克隆的鸡痘病毒（ＦＰＶ）２８２Ｅ＿４株基因组ＴＫ基因，在ＮｃｏⅠ部位引入痘苗病毒Ｐ７．５启动子和Ｐ１１启动子控制下的大肠杆菌ＬａｃＺ基因，经酶切鉴定，成功地构建了用于ＦＰＶ重组的载体质粒。与亲本毒株在鸡胚成纤维细胞内共转染，产生了表达ＬａｃＺ基因的重组鸡痘病毒。经传代筛选、纯化，重组病毒较稳定。实验结果表明，以ＴＫ基因构建的重组载体质粒可用于外源基因的重组表达研究。     

鸭流感病毒4/2004-H6N2亚型毒株的血凝素基因全序列克隆与分析

2004初从正常鸭群中分离到一株鸭源禽流感病毒，命名为A／Duck／HN／4／2004(H6N2)。经对血凝素基因(HA)序列分析发现HA基因全长为1744bp，共编码566个氨基酸，在裂解位点仅含一个碱性氨基酸-精氨酸(R)，符合LPAIV的标准。将所得基因序列与已发表的同一亚型参考序列分析表明，与H6亚型流感HA基因同源性为89．2％-97．1％，经分子遗传演化分析表明本次分离株与香港分离株A／Duck／Hong Kong／3600／99(H6N2)、A／Duck／Hong Kong／3600／99(H6N2)最近。     

Quantifying landscape linkages among giant panda subpopulations in regional scale conservation

Understanding habitat requirements and identifying landscape linkages are essential for the survival of isolated populations of endangered species. Currently, some of the giant panda populations are isolated, which threatens their long-term survival, particularly in the Xiaoxiangling mountains. In the present study, we quantified niche requirements and then identified potential linkages of giant panda subpopulations in the most isolated region, using ecological niche factor analysis and a least-cost path model. Giant pandas preferred habitat with conifer forest and gentle slopes (>20 to ≤30°). Based on spatial distribution of suitable habitat, linkages were identified for the Yele subpopulation to 4 other subpopulations (Liziping, Matou, Xinmin and Wanba). Their lengths ranged from 15 to 54 km. The accumulated cost ranged from 693 to 3166 and conifer forest covered over 31%. However, a variety of features (e.g. major roads, human settlements and large unforested areas) might act as barriers along the linkages for giant panda dispersal. Our analysis quantified giant panda subpopulation connectivity to ensure long-term survival.     

宰前短期添加镁对PIC育肥猪肉色和pH值的影响

试验研究了宰前短期添加不同剂量镁对PIC育肥猪肉质的影响。试验以PIC育肥猪为研究对象,共分4个处理,每个处理4头。分别在限饲基础日粮基础上每日补饲0、2、4、6 g镁。结果表明:每头添加镁2 g/d有提高色素含量趋势(P>0.05),添加4 g/d可极显著提高肌肉色素含量7.41%(P<0.01),添加6 g/d显著提高肌肉色素含量(P<0.05);肉色评分随添加剂量的增加而提高,2 g/d可以显著提高肉色评分(P<0.05),4、6 g/d可极显著提高肉色评分(P<0.01);随镁添加量的增加,肌肉pH值显著提高(P<0.05),6 g/d添加组与对照组相比较差异极显著(P<0.01)。宰前5 d补镁可增加肉色,显著提高肌肉pH值,改善猪肉品质。     

谈谈"一犬事多主"的训练

“一犬事多主”是指经过专门训练的一头工作犬,能够忠诚、勇敢地为一人乃至多人服务。这一训练方法,虽然与传统观念上的“义犬不事二主”相矛盾,但是客观上已经证实其有可行性。对于“义犬不事二主”的观念,笔者认为:虽然突出了犬的忠义品质,但是容易误导人们,将那些能为多个主     

Issues and special features of animal health research

ABSTRACT: In the rapidly changing context of research on animal health, INRA launched a collective discussion on the challenges facing the field, its distinguishing features, and synergies with biomedical research. As has been declared forcibly by the heads of WHO, FAO and OIE, the challenges facing animal health, beyond diseases transmissible to humans, are critically important and involve food security, agriculture economics, and the ensemble of economic activities associated with agriculture. There are in addition issues related to public health (zoonoses, xenobiotics, antimicrobial resistance), the environment, and animal welfare.Animal health research is distinguished by particular methodologies and scientific questions that stem from the specific biological features of domestic species and from animal husbandry practices. It generally does not explore the same scientific questions as research on human biology, even when the same pathogens are being studied, and the discipline is rooted in a very specific agricultural and economic context.Generic and methodological synergies nevertheless exist with biomedical research, particularly with regard to tools and biological models. Certain domestic species furthermore present more functional similarities with humans than laboratory rodents.The singularity of animal health research in relation to biomedical research should be taken into account in the organization, evaluation, and funding of the field through a policy that clearly recognizes the specific issues at stake. At the same time, the One Health approach should facilitate closer collaboration between biomedical and animal health research at the level of research teams and programmes.     

藏北高原高寒草地土壤有效养分含量研究

选择藏北高原高寒草地不同海拔的12个样地对土壤有效养分进行研究,结果表明:在海拔4 480m优势植物为嵩草(Kobresiaspp)、苔草(Carex tristachya)的高山草原土速效氮含量较高;人工种植的在海拔4 530m的紫花苜蓿(Medicago sativa)和海拔4 300m燕麦草(Arrhenatherum elatius)有效磷含量较高;高山草原土壤速效钾含量较高;在海拔4 466m优势植物为早熟禾(Poasp.)的高山草原土有效铜和有效铁含量较高;在海拔4 481m高山草甸土的裸地有效锰含量较高;在海拔3 473m原生植被覆盖的亚高山草原土有效锌含量较高。有效氮、磷、铜、锌和锰在高山草原土中分布异质性较亚高山草原土、高山草甸土和高山荒漠土差;速效钾在各种类型土壤中分布异质性较均匀;有效铜在亚高山草原土中分布异质性较差。总体而言,藏北高原高寒草地中土壤有效养分含量高低和分布异质性,受到海拔高度、土壤质地、地上植被、人为开发利用及气候变化等多种因素影响。     

激情泰国魅力VIV——聚焦2009亚洲国际集约化畜牧展览会

2009年3月11日,亚太地区规模最大的国际性畜牧展览会VIV Asia2009,在泰国首都曼谷国际贸易展览中心（BITEC）开幕,会期3天。展会同期召开了畜牧水产行业的系列研讨会和技术讲座。VIV是荷兰VNU皇家展览集团旗下的“国际集约化畜牧业展览会”品牌,与全球各地合作形成了VIV Eu-rope（欧洲）、VIV Asia（亚洲）、VIV China（中国）、VIV Turkey（土耳其）、VIV Russia（俄罗斯）、VIV India（印度）等系列品牌展会,在全球畜牧行业具有很大的影响力和号召力。VIV Asia是VUN与泰国NEO展览集团于1993年开始合作开办的亚洲国际集约化畜牧展览会,每两年一届,举办地固定在泰国曼谷。     

努力学习把握重点认真贯彻全国畜牧总站站长

农业部饲料法规宣贯暨行业统计培训班今天开班了,首先,我代表全国畜牧总站、中国饲料工业协会对会议的成功召开表示热烈的祝贺!刚才,王宗礼副司长代表农业部畜牧业司(全国饲料工作办公室)全面介绍了当前饲料行业发展形势,明确了本次培训班的主要任务,并就贯彻实施<饲料生产企业审查办法>和做好饲料行业信息统计工作做了重要讲话.希望大家认真学习并在实际工作中认真贯彻落实.下面,我讲三点意见,供参考.