环境因素对球虫卯囊孢子化的影响

探讨环境因素对球虫卵囊孢子化的影响,为切断鸡球虫病的感染途径提供依据。本实验将未孢子化球虫卵囊分别置于高温干燥、高温潮湿、室温三个不同环境中处理后,再按常规方法培养,并测其孢子化率。结果表明:80℃高温干燥环境中放置15 min以上,80℃高温潮湿环境中放置30 min以上,室温环境中放置1 d以上,卵囊均已完全丧失孢子化能力。     

预防兽医资源库的构建与学生实践能力的培养

预防兽医资源库的构建,建立了以六门课程为主干,以文献资料、互动网站为依托,以素材库、课件库、网络课程案例库、试题库为内容,形成综合性教学体系。视频图像的引入,增强了学生对实践教学的预知和理解能力,大大地提高了实践教学的效果,同时增加了实践教学的容量,培养了学生的实践能力。     

苏丹红和饲料着色剂的区别

本文主要从苏丹红和饲料着色剂的化学结构、作用机理及毒性等方面说明饲料着色剂与苏丹红的天壤之别。     

在广东省饲料行业协会第四次会员代表大会上的讲话

广东省饲料行业协会第四次会员代表大会的召开,是广东省饲料界的一件喜庆事,也是我们全饲料行业的一件喜庆事。我谨代表全国畜牧总站、中国饲料工业协会表示热烈的祝贺!热烈祝贺通过选举产生的广东省饲料行业协会新一届领导集体!热烈祝贺广东省饲料工业取得的巨大成就!热烈祝贺广东省饲料工业协会取得的出色业绩!热烈祝贺广东饲料行业对养殖业增产增效、对农民增收、对农业和农村经济建设和社会主义新农村建设做出的突出贡献!     

复方中草药免疫增强剂对鸡堆型艾美耳球虫早熟株免疫的影响研究

选用两个中草药方剂,采用1.5%、2.0%两个添加水平,在相同方法、相同攻虫强度下,比较其抗鸡堆型艾美耳球虫的效果。结果表明:方剂一在2.0%的添加水平时效果显著,抗球虫指数高达196.04,其E-玫瑰花环形成率也显著提高,具有明显的免疫促进作用。     

Differential expression of peroxisome proliferator-activated receptors alpha and gamma gene in various chicken tissues

The peroxisome proliferator-activated receptors (PPARs) are the members of superfamily of nuclear hormone receptors. A great number of studies in rodent and human have shown that PPARs were involved in the lipids metabolism. The goal of the current study was to investigate the expression pattern of PPAR genes in various tissues of chicken. The tissue samples (heart, liver, spleen, lung, kidney, stomach, intestine, brain, breast muscle and adipose) were collected from six Arber Acres broilers (8 weeks old, male and female birds are half and half). Semi-quantitative RT-PCR and Northern blot were used to characterize the expression of PPAR-alpha and PPAR-gamma genes in the above tissues. By semi-quantitative RT-PCR, the results showed the expression level of PPAR-alpha gene was higher in brain, lung, kidney, heart and intestine, medium in stomach, liver and adipose than in spleen, and it did not express in breast muscle. The expression level of PPAR-gamma gene was higher in adipose, medium in brain and kidney than in spleen, heart, lung, stomach and intestine, but it did not express in liver and breast muscle. Northern blot results showed that PPAR-alpha gene expressed in heart, liver, kidney and stomach, and the intensity of hybridization signal was the stronger in liver and kidney than in other tissues, however, PPAR-gamma gene only expressed in adipose and kidney tissues. The results of this study showed the profile of PPAR gene expression in the chicken was similar to that in rodent, human and pig. However the expression profile of chicken also have its own specific trait, i.e. compared with mammals, PPAR-alpha gene can not be detected in skeletal muscle and PPAR-gamma gene can be stronger expressed in kidney tissues. This work will provide some basic data for the PPAR genes expression and lipids metabolism of birds.     

Characterization of a monoclonal antibody to Spiroplasma eriocheiris and identification of a motif expressed by the pathogen

Tremor disease (TD) has been found to be a lethal epidemic in Chinese mitten crab during recent years. A new species of spiroplasma, Spiroplasma eriocheiris (S. eriocheiris), was identified as the pathogen causing TD. In order to acquire appropriate biological tools for characterizing this newly discovered pathogen, we developed a new S. eriocheiris specific monoclonal antibody (mAb) 6H7. The antibody showed high binding affinity to S. eriocheiris (K_a = 6.25 × 10⁸ M^?1) and it bound to the adhesin-like protein (ALP41) of S. eriocheiris in Western blot analysis. Screening of a commercially available 12-mer linear peptide library by using 6H7 as bait led to isolation of a consensus sequence (FQGINHYNQMER). The clone displaying this sequence exhibited a significant, dose-dependent binding to 6H7 and inhibited the binding of S. eriocheiris to 6H7, suggesting a similar epitope between the sequence and S. eriocheiris. Homology searches and multiple sequence alignments indicated moderate homology between the consensus peptides and the ALP of S. eriocheiris. Thus, an internal image of 6H7 binding epitope might be buried in ALP41 of S. eriocheiris. In conclusion, 6H7 is a promising mAb to identify S. eriocheiris and the consensus sequence can be used in future research on the characterization of S. eriocheiris and its pathogenesis.     

氟对牛脾淋巴细胞膜ATP酶活性的影响

取健康牛脾脏,常规方法分离培养淋巴细胞,用不同浓度氟化钠（NaF）染毒24h。低渗溶胞法提取细胞膜,检测膜上ATP酶活性。结果显示,与对照组相比较,染氟组淋巴细胞膜ATP酶活性降低,组间差异显著或极显著。表明：氟能抑制牛脾淋巴细胞膜ATP酶活性。     

细粒棘球蚴细胞系(13G-5)细胞代谢抗原的免疫原性和反应原性

应用培育成功的人源细粒棘球蚴细胞系(13G-5)细胞的天然代谢抗原,接种昆明小鼠,于接种后第14天给免疫小鼠接种人源细粒棘球蚴原头节约1 000 个,于200 d 剖检观察有无包囊形成,病理切片鉴定;并用该抗原作为诊断抗原,使用间接血凝(IHA)、琼脂扩散(AGD)试验,检测临床确诊的细粒棘球蚴病人血清。以多房棘球蚴病人血清、细颈囊尾蚴羊血清及正常人血清作为对照。结果表明,该抗原能使60% 的小鼠获得完全抵抗细粒棘球蚴原头节攻击的能力,未完全获得保护的小鼠形成包囊的数量、大小较对照组明显减少,且为不育囊;使用该抗原IHA 检测31 份血清,阳性24 份,阳性率77.42% ,AGD检测30 份血清,阳性13 份,阳性率43.33% ,2 种方法对多房棘球蚴病人血清和细颈囊尾蚴羊血清及正常人血清不起反应。     

高温条件下湿度对肉用仔鸡干物质和能量采食量及代谢率的影响

分别用4～5周龄艾维茵肉用仔鸡216只和6～7周龄180只分两阶段进行了试验,第1阶段同在32℃气温下设置30％,60％,90％相对湿度,第2阶段,同在30℃气温下设置30％,60％,90％相对湿度,分别测定对干物质及能量的采食量和表观代谢率.每个处理设6个重复,每阶段试验期为2周.结果表明,高温条件下,相对湿度显著影响4～5周龄肉仔鸡的干物质采食量和能量采食量(P<0．05),而对6～7周龄肉鸡无显著影响;高温条件下6～7周龄高湿组的干物质代谢率和能量代谢率较高(P<0．05),而4～5周龄阶段这一差异不显著