Health survey of wild and captive bog turtles (Clemmys muhlenbergii) in North Carolina and Virginia.

Blood samples, fecal samples, and cloacal swabs were collected from 42 bog turtles (Clemmys muhlenbergii). including 14 wild males, 22 wild females, three captive males, and three captive females, in Virginia and North Carolina, USA. Samples were analyzed for hematologic and plasma chemistry values, Mycoplasma sp. antibodies, intestinal parasites, and normal cloacal flora.     

Integrating revenues from carbon sequestration into economic breeding objectives for Eucalyptus globulus pulpwood production

A system where carbon sequestration was directly dependent upon biomass production in a plantation was modelled to assess whether economic breeding objectives for the genetic improvement of Eucalyptus globulus were sensitive to potential revenues from carbon sequestration. Carbon dioxide equivalent accumulation in the biomass (CO₂e) of the Australian E. globulus plantation estate established between 2004 and 2012 was estimated. Total carbon dioxide equivalent (CO₂e) accumulation was in the order of ～146 t CO₂e ha^?1, of which 62 t CO₂e ha^?1 were tradable in 2012 (the 1st Kyoto Protocol commitment period) and a further 30 t CO₂e ha^?1 were tradable in 2016 (a hypothetical second Kyoto protocol commitment period). The correlated response of breeding objectives with and without carbon revenues (ΔcG _{h 1}) never fell below 0.86 in sensitivity analysis, and the mean was 0.93. Where economic breeding objectives for the genetic improvement of Eucalyptus globulus for pulpwood plantations are based on maximizing net present value by increasing biomass production, the consideration of carbon revenues in economic breeding objectives will have no significant effect on the relative economic weights of the key economic traits, wood basic density and standing volume at harvest.     

Foliage browning and shoot death in Sitka spruce and other conifers in northern Britain during winter 1983–84

A hitherto undescribed form of injury to Sitka spruce and other conifers occurred in plantations throughout northern Britain during the dormant season 1983–84. Damage consisted primarily of shoot death which was typically concentrated in a zone of variable extent below the upper few whorls of the tree. It occurred predominantly in forests in the west and was most severe in older crops at high elevation. Possible causes are discussed and it is concluded that the damage was probably climatic in origin.     

Costs of harvesting, transportation and milling in the Brazilian Amazon: Estimation and policy implications

We estimate economic cost functions for timber harvesting, transportation and milling in the Brazilian Amazon using a 2003 sample of 527 firms in both new and older frontier locations. We find that labor wage, distance from the forest to the processing location, type of equipment, and the type of the frontier all factor significantly in the total and marginal cost of each activity, and that predicted processing costs are not significantly different on new frontiers implying a lack of technology adoption as industry expansion into the Amazon has occurred. We also show that capturing economies of scale in logging by increasing average annual logging volumes by 50% and reducing the number of firms to about 1400 could lead to an industry wide cost savings of approximately US$90 million per year. Similar economies of scale are also present in log transport but not in processing. Further, if improved logging techniques allow harvest for an additional 1 month per year, for example through better planning, the industry could reduce logging costs by almost US$30 million. This points towards generating forest policies and economic conditions that encourage firm size growth, as opposed to those policies encouraging massive entry of small, unregulated and inefficient firms, and the adoption of management practices that allow for additional time in the forest.     

Pedigree analysis of the origin of manganese tolerance in Canadian spring wheat (Triticum aestivum L.) cultivars

Summary Breeding wheat (Triticum aestivum L.) for tolerance to manganese (Mn) might be in some cases more feasible and economical than use of soil amendments. As part of research on the heritability of Mn tolerance, a study on the level of Mn tolerance in Canadian wheat cultivars and its probable origin was accomplished by analysis of cultivar pedigrees and drawing phylogenetic maps to discern filial relationships. Cultivar tolerance to Mn was determined by relative root weight (RRW) in solution culture in the presence of 500 M Mn. A total of 91 cultivars were screened, 76 of which were Canadian. These data, together with data from another 28 cultivars reported in the literature, were used to draw two pedigree maps, a map for Canadian cultivars only, and a map for the Mn-tolerant Canadian cultivars Norquay and Laura. Results indicated a range of tolerance to Mn among Canadian cultivars. Manganese tolerance, found in either Canadian or foreign germplasm, and of either recent or older selection or origin, seems to have originated from land races from Rio Grande do Sul, the southernmost state of Brazil. Tolerance may have been introduced into Canadian germplasm directly by the use of Brazilian cultivars as parents, or indirectly by the introduction of Mexican germplasm with Brazilian parentages. This information will help the plant breeder to develop plant breeding systems, and may also help in the study of the mechanisms for Mn tolerance in wheat.     

Genetic diversity and conservation of two endangered eggplant relatives (<Emphasis Type="Italic">Solanum vespertilio</Emphasis> Aiton and <Emphasis Type="Italic">Solanum lidii</Emphasis> Sunding) endemic to the Canary Islands

Solanum vespertilio Aiton and Solanum lidii Sunding are endemic, endangered wild species from the Canary Islands. These species are of potential value for eggplant (S. melongena) breeding, given that they are part of the secondary genepool of this crop. We study genetic diversity with amplified fragment length polymorphisms (AFLPs) markers from 5 populations of S. vespertilio (47 samples) and 3 of S. lidii (26 samples). Five related African species (S. dasyphyllum Schumach. et Thonn., S. delagoense Dunal, S. campylacanthum Hochst., S. panduriforme E. Mey, S. aff. violaceum Ortega) were also included in the analysis. A total of 235 AFLP markers included 178 and 156 that were polymorphic in S. vespertilio and S. lidii, respectively. Analysis of genetic distance, phenograms, and principal component plots showed that these rare Canarian species are differentiated (G _ST = 0.412) from the continental materials and that Solanum vespertilio is more distinct to its African congeners than is S. lidii. There is a relatively high level of differentiation between the two species (G _ST = 0.373), that presumably reflects geographic restrictions (S. lidii to Gran Canaria; S. vespertilio essentially to Tenerife). However, both species have similar levels of total diversity. We speculate that the combination of the many unusual reproductive features (andromonoecy, zygomorphy, heteranthery and weak enantiostyly in S. vespertilio) help explain genetic diversity that is high for self compatible species. The high genetic diversity may also indicate populations were larger in the past. A decrease in population size could contribute to the relatively low genetic differentiation among the populations. The data presented herein provide the foundation for initiation of ex situ and in situ conservation programs for these wild relatives of eggplant. This paper is dedicated to the memory of Dr. Richard N. Lester, who made significant contributions to the taxonomy, biosystematics and conservation of genetic resources of African species of Solanum.     

Assessing Se cycling and toxicity in aquatic ecosystems

Toxic substances exert impacts on ecosystems at several levels: biogeochemical, toxicological, and populations/communities. Integrating exposure (biogeochemistry) and effects (toxicology) into an ecological context requires models as a necessary step to prediction and assessment of effects on populations and their interactions. To this end, research is being conducted to develop techniques for predicting the effects of Se on aquatic ecosystems. The objective is to develop mechanistic models to predict biogeochemical cycling, toxicological processes, and toxic effects on population and community dynamics. Earlier research demonstrated that different chemical forms of Se have different toxicological properties. Biogeochemical research culminated with a dynamic model of the Se cycle. In ongoing research, experiments are being conducted on microorganisms, phytoplankton, microzooplankton, zooplankton, benthic invertebrates, and fish to measure uptake, biotransformation, and depuration kinetics of different chemical forms of Se, transfer through the food web, and effects on growth, reproduction, community interactions, and survival. This information will provide the basis for the ecosystem effects model.     

Selenium accumulation in beef: effect of dietary selenium and geographical area of animal origin

Selenium (Se) is an essential nutrient with multiple human health benefits; the single most important dietary source of Se is beef. The Se content of beef varies, and cattle fed a high selenium diet may have Se concentrations in beef that are well above average. Such beef is potentially a unique supplemental source of dietary Se. To examine factors affecting Se accumulation in beef, 16 steers (initial wt 374.4 +/- 33.7 kg) were taken from seleniferous or nonseleniferous areas and fed in a 2 x 2 factorial design with diets high or moderate in Se (11.9 or 0.62 mg Se/kg diet). Diets contained 50% alfalfa, 25% wheat, and 25% corn on a dry matter basis. All dietary Se was from agricultural products, and Se in the high Se diet was primarily from high Se wheat and alfalfa hay. A loin muscle biopsy was taken at the start of the trial to determine initial Se content of beef. Steers were slaughtered after 14 weeks of the trial, and edible carcass (round, sirloin, shoulder clod, and ribeye) and organ samples were collected. Diets did not affect growth or feed intake (P > 0.05), and Se toxicity signs were not observed. Different cuts of meat had similar Se concentrations, and the Se content of all cuts was increased by both high dietary Se and high Se background. Except for liver and kidney, Se in tissues was increased by seleniferous background (P < 0.02) and high dietary Se (P < 0.001). Kidney Se concentrations of animals fed the high Se diet were lowest in animals from seleniferous areas (P = 0.04), suggesting a possible adaptation to the high Se diet. These results demonstrate that cattle fed diets high in Se from agricultural products will accumulate substantial amounts of Se in the beef without developing signs of Se toxicity and that prior Se status regulates Se accumulation in some organs. They further demonstrate that management practices may be altered so as to make beef a significant source of dietary Se.     

Flavor encapsulation in yeasts: limonene used as a model system for characterization of the release mechanism

Empty yeast cells are used as a new delivery system for flavor encapsulation. The flavor release mechanism from yeast cells is characterized using a series of analytical techniques, and limonene is used as a model representing a hydrophobic flavor. Furthermore, the thermal stability of the capsules was assessed. The characterization of the cell wall structure gives rise to the development of an empirical model explaining water adsorption as well as the desorption singularities observed on drying. The study of the rate of flavor release as a function of temperature and water uptake in the cell wall clearly demonstrated a particular behavior of the yeast cell wall permeability. Below a water activity around 0.7, no flavor release is permitted whereas release occurs above it. Surface analysis on dry or wet cells using atomic force microscopy is discussed.