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排序方式: 共有1484条查询结果,搜索用时 15 毫秒
941.
Joo Youn Park Lawrence K. Fox Keun Seok Seo Mark A. McGuire Yong Ho Park Fred R. Rurangirwa William M. Sischo Gregory A. Bohach 《Veterinary microbiology》2011,147(1-2):149-154
The coagulase-negative staphylococci (CNS) are the most prevalent mastitis pathogen group yet their virulence characteristics have not been well described. We investigated the presence of 19 classical and newly described staphylococcal superantigen (SAg) genes in CNS isolates from bovine intramammary infections (IMI). A total of 263 CNS representing 11 different Staphylococcus spp. were examined, and 31.2% (n = 82) of CNS isolates had one or more SAg genes; there were 21 different SAg gene combinations. The most prevalent combination of SAg genes (seb, seln and selq; n = 45) was found in S. chromogenes, S. xylosus, S. haemolyticus, S. sciuri subsp. carnaticus, S. simulans and S. succinus. The genes for SAgs appear to be widely distributed amongst CNS isolated from bovine IMI. 相似文献
942.
John L. Orrock Gregory R. Curler Brent J. Danielson David R. Coyle 《Landscape Ecology》2011,26(10):1361-1372
The size, shape, and isolation of habitat patches can affect organism behavior and population dynamics, but little is known
about the relative role of shape and connectivity in affecting ecological communities at large spatial scales. Using six sampling
sessions from July 2001 until August 2002, we collected 33,685 arthropods throughout seven 12-ha experimental landscapes consisting
of clear-cut patches surrounded by a matrix of mature pine forest. Patches were explicitly designed to manipulate connectivity
(via habitat corridors) independently of area and edge effects. We found that patch shape, rather than connectivity, affected
ground-dwelling arthropod richness and beta diversity (i.e. turnover of genera among patches). Arthropod communities contained
fewer genera and exhibited less turnover in high-edge connected and high-edge unconnected patches relative to low-edge unconnected
patches of similar area. Connectivity, rather than patch shape, affected the evenness of ground-dwelling arthropod communities;
regardless of patch shape, high-edge connected patches had lower evenness than low- or high-edge unconnected patches. Among
the most abundant arthropod orders, increased richness in low-edge unconnected patches was largely due to increased richness
of Coleoptera, whereas Hymenoptera played an important role in the lower evenness in connected patches and patterns of turnover.
These findings suggest that anthropogenic habitat alteration can have distinct effects on ground-dwelling arthropod communities
that arise due to changes in shape and connectivity. Moreover, this work suggests that corridors, which are common conservation
tools that change both patch shape and connectivity, can have multiple effects on arthropod communities via different mechanisms,
and each effect may alter components of community structure. 相似文献
943.
944.
Bacteria that selectively kill males ("male-killers") were first characterized more than 50 years ago in Drosophila and have proved to be common in insects. However, the mechanism by which sex specificity of virulence is achieved has remained unknown. We tested the ability of Spiroplasma poulsonii to kill Drosophila melanogaster males carrying mutations in genes that encode the dosage compensation complex. The bacterium failed to kill males lacking any of the five protein components of the complex. 相似文献
945.
Xu J Peng C Sankaran VG Shao Z Esrick EB Chong BG Ippolito GC Fujiwara Y Ebert BL Tucker PW Orkin SH 《Science (New York, N.Y.)》2011,334(6058):993-996
Persistence of human fetal hemoglobin (HbF, α(2)γ(2)) in adults lessens the severity of sickle cell disease (SCD) and the β-thalassemias. Here, we show that the repressor BCL11A is required in vivo for silencing of γ-globin expression in adult animals, yet dispensable for red cell production. BCL11A serves as a barrier to HbF reactivation by known HbF inducing agents. In a proof-of-principle test of BCL11A as a potential therapeutic target, we demonstrate that inactivation of BCL11A in SCD transgenic mice corrects the hematologic and pathologic defects associated with SCD through high-level pancellular HbF induction. Thus, interference with HbF silencing by manipulation of a single target protein is sufficient to reverse SCD. 相似文献
946.
Abrahamsen MS Templeton TJ Enomoto S Abrahante JE Zhu G Lancto CA Deng M Liu C Widmer G Tzipori S Buck GA Xu P Bankier AT Dear PH Konfortov BA Spriggs HF Iyer L Anantharaman V Aravind L Kapur V 《Science (New York, N.Y.)》2004,304(5669):441-445
The apicomplexan Cryptosporidium parvum is an intestinal parasite that affects healthy humans and animals, and causes an unrelenting infection in immunocompromised individuals such as AIDS patients. We report the complete genome sequence of C. parvum, type II isolate. Genome analysis identifies extremely streamlined metabolic pathways and a reliance on the host for nutrients. In contrast to Plasmodium and Toxoplasma, the parasite lacks an apicoplast and its genome, and possesses a degenerate mitochondrion that has lost its genome. Several novel classes of cell-surface and secreted proteins with a potential role in host interactions and pathogenesis were also detected. Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development. 相似文献
947.
948.
He L Lu XY Jolly AF Eldridge AG Watson SJ Jackson PK Barsh GS Gunn TM 《Science (New York, N.Y.)》2003,299(5607):710-712
mahoganoid is a mouse coat-color mutation whose pigmentary phenotype and genetic interactions resemble those of Attractin (Atrn). Atrn mutations also cause spongiform neurodegeneration. Here, we show that a null mutation for mahoganoid causes a similar age-dependent neuropathology that includes many features of prion diseases but without accumulation of protease-resistant prion protein. The gene mutated in mahoganoid encodes a RING-containing protein with E3 ubiquitin ligase activity in vitro. Similarities in phenotype, expression, and genetic interactions suggest that mahoganoid and Atrn genes are part of a conserved pathway for regulated protein turnover whose function is essential for neuronal viability. 相似文献
949.
950.
Kuhlman B Dantas G Ireton GC Varani G Stoddard BL Baker D 《Science (New York, N.Y.)》2003,302(5649):1364-1368
A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue alpha/beta protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature. 相似文献