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91.
Fauci AS Johnston MI Dieffenbach CW Burton DR Hammer SM Hoxie JA Martin M Overbaugh J Watkins DI Mahmoud A Greene WC 《Science (New York, N.Y.)》2008,321(5888):530-532
The need to broaden research directed at answering fundamental questions in HIV vaccine discovery through laboratory, nonhuman primate (NHP), and clinical research has recently been emphasized. In addition, the importance of attracting and retaining young researchers, developing better NHP models, and more closely linking NHP and clinical research is being stressed. In an era of a level budget for biomedical research at the U.S. National Institutes of Health (NIH), HIV/AIDS vaccine research efforts will need to be carefully prioritized such that resources to energize HIV vaccine discovery can be identified. This article summarizes progress and challenges in HIV vaccine research, the priorities arising from a recent summit at NIAID, and the actions needed, some already under way, to address those priorities. 相似文献
92.
Lamont LA Greene SA Grimm KA Tranquilli WJ 《American journal of veterinary research》2004,65(1):93-98
OBJECTIVE: To determine the relationship between bispectral index (BIS) and minimum alveolar concentration (MAC) multiples of sevoflurane in cats. ANIMALS: 8 domestic cats. PROCEDURE: Each cat was anesthetized twice with sevoflurane. First, the MAC of sevoflurane for each cat was determined by use of the tail clamp method. Second, cats were anesthetized with sevoflurane at each of 5 MAC multiples administered in random order. Ventilation was controlled, and after a 15-minute equilibration period at each MAC multiple of sevoflurane, BIS data were collected for 5 minutes and the median value of BIS calculated. RESULTS: The mean (+/- SD) MAC of sevoflurane was 3.3 +/- 0.2%. The BIS values at 0.5 MAC could not be recorded as a result of spontaneous movement in all 8 cats. The BIS values at 2.0 MAC were confounded by burst suppression in all 8 cats. Over the range of 0.8 to 1.5 MAC, BIS values decreased significantly with increasing end-tidal sevoflurane concentrations. Mean (+/- SD) BIS measurements were 30 +/- 3, 21 +/- 3, and 5 +/- 2 at 0.8, 1.0, and 1.5 MAC, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Values of BIS are inversely and linearly related to end-tidal sevoflurane concentrations in anesthetized cats, and BIS may be a useful predictor of CNS depression in this species. The consistently low BIS values recorded in this study suggest that clinical BIS end points used to titrate anesthetic agents in humans may not be applicable to cats. 相似文献
93.
Leonardo F.C. Brito DVM PhD Patricia L. Sertich MS VMD Keith Durkin BSc Bhanu P. Chowdhary BVScAH PhD Regina M. Turner VMD PhD Lauren M. Greene VMD Sue McDonnell PhD 《Journal of Equine Veterinary Science》2008,28(7):431-436
A 19-month-old Standardbred colt was donated to the University of Pennsylvania School of Veterinary Medicine with a suspicion of intersexuality. The anal−genital distance and penis were normal, and there was no evidence of intersexuality, but the colt was bilaterally cryptorchid. Several aspects of the colt's behavior appeared unusual, including general temperament and behavior described as sympathetically dull and affable. With herd mates, the colt appeared slow to perceive or to learn the usual intraspecies social cues. An atypical gait characterized by intermittent unnatural shuffle of the hind limbs, sliding them along in short rhythmic strides for 3 to 10 seconds at a time was noted at times when a horse might normally transition from a slow walk to a fast walk or a slow trot. Occasionally the colt exhibited slight protrusion of the tongue through the teeth and lips with jaw movements and smacking of the tongue against the teeth as if struggling to retract the tongue to the normal position. Evaluation of the karyotype combined with fluorescent in situ hybridization (FISH) revealed an abnormal male karyotype showing trisomy of chromosome 27 (65, XY + 27). The colt was euthanized at 24 months of age, and a necropsy revealed no significant abnormalities. This case of trisomy was not associated with developmental abnormalities described in other rare reports of trisomy in horses; however, some features were strikingly similar to that of humans with trisomy 21. FISH was demonstrated to be an excellent method for correct identification of equine chromosomes. 相似文献
94.
Hess SL Henry RM Leovy CB Ryan JA Tillman JE Chamberlain TE Cole HL Dutton RG Greene GC Simon WE Mitchell JL 《Science (New York, N.Y.)》1976,194(4260):78-81
The results from the meteorology instruments on the Viking 1 lander are presented for the first 20 sols of operation. The daily patterns of temperature, wind, and pressure have been highly consistent during the period. Hence, these have been assembled into 20-sol composites and analyzed harmonically. Maximum temperature was 241.8 degrees K and minimum 187.2 degrees K. The composite wind vector has a mean diurnal magnitude of 2.4 meters per second with prevailing wind from the south and counterclockwise diurnal rotation. Pressure exhibits diurnal and semidiurnal oscillations. The diurnal is ascribed to a combination of effects, and the semidiurnal appears to be the solar semidiurnal tide. Similarities to Earth are discussed. A major finding is a continual secular decrease in diurnal mean pressure. This is ascribed to carbon dioxide deposition at the south polar cap. 相似文献
95.
Krishona Lynn Martinson Thomas Bartholomay Kathleen P. Anderson Christine Skelly Elizabeth Greene 《Journal of Equine Veterinary Science》2012
Evaluation has become a more significant component of planning and delivering extension programs, as federal partners and granting agencies are requesting information on program and integrated grant outcomes, including participant learning gains, behavior change, and program-generated impacts. Effective evaluation of equine extension programs involves a balance between asking enough well-designed questions to obtain desired information and keeping the evaluation tool brief enough to encourage participant completion. For most faculties, the difficulty with evaluation lies in developing appropriate and useful questions. The objective of this article was to share examples of questions successfully used to evaluate six key equine extension program areas: participant demographics, program logistics, participant behavior change, participant knowledge gain, teaching effectiveness, and program impact. Data generated by postprogram evaluations can be a source of statistically sound information that can be shared with administration, stakeholders, and granting agencies. Extension personnel can use evaluation data to improve planning and delivery of extension programs and to demonstrate teaching ability and program impacts. 相似文献
96.
97.
The Fick and thermodilution (TD) methods are two currently popular techniques for determination of cardiac output (CO) in adult horses. To our knowledge, a comparison of these two techniques has not been reported. Six healthy, resting, fit, adult horses of either sex and weighing 516.5+/-33.2 kg (mean+/-SD) were instrumented to enable measurement of cardiac output. Resting CO was determined by the Fick method and by thermodilution while the horses stood quietly in the stocks. Fick and thermodilution CO measurements were repeated under conditions of increased cardiac output achieved with the use of a dobutamine infusion (5 microg kg(-1) min(-1), IV), and again under conditions of decreased CO induced by administration of xylazine (0.5 mg/kg, IV). Fick and thermodilution cardiac outputs were compared using Bland-Altman analysis for repeated measures. The mean of the differences+/-1.96SD (bias and precision) between the two techniques was 1.88+/-24.17 L/min. Variability between measurements with the two techniques was decreased to 3.41+/-46.78 mL kg(-1) min(-1) when CO was normalized for body size by calculation of cardiac index. 相似文献
98.
Linking winter and summer events in a migratory bird by using stable-carbon isotopes 总被引:1,自引:0,他引:1
For migratory birds, early arrival and physical condition on the breeding grounds are important determinants of reproductive success and fitness. Differences in arrival times often exceed a month, and later arriving individuals are often in poorer condition. Habitat-specific isotopic signatures indicate that the quality of winter habitats occupied by American redstarts (Setophaga ruticilla) determines their physical condition and spring departure dates, which in turn result in variable arrival schedules and condition on temperate breeding grounds. These findings link events in tropical winter grounds with those in temperate breeding areas for a migratory songbird and provide evidence that winter habitats may be limiting. 相似文献
99.
100.
Greene K 《Science (New York, N.Y.)》2003,301(5631):301-302