全文获取类型
收费全文 | 603篇 |
免费 | 44篇 |
专业分类
林业 | 39篇 |
农学 | 32篇 |
基础科学 | 2篇 |
103篇 | |
综合类 | 16篇 |
农作物 | 30篇 |
水产渔业 | 80篇 |
畜牧兽医 | 289篇 |
园艺 | 17篇 |
植物保护 | 39篇 |
出版年
2023年 | 10篇 |
2022年 | 14篇 |
2021年 | 25篇 |
2020年 | 43篇 |
2019年 | 36篇 |
2018年 | 28篇 |
2017年 | 24篇 |
2016年 | 41篇 |
2015年 | 30篇 |
2014年 | 32篇 |
2013年 | 45篇 |
2012年 | 59篇 |
2011年 | 56篇 |
2010年 | 30篇 |
2009年 | 29篇 |
2008年 | 24篇 |
2007年 | 26篇 |
2006年 | 21篇 |
2005年 | 15篇 |
2004年 | 12篇 |
2003年 | 11篇 |
2002年 | 6篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1986年 | 1篇 |
1981年 | 1篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有647条查询结果,搜索用时 78 毫秒
71.
Gabriela Noronha de Toledo Pamela Rodrigues Reina Moreira Daniele Santos Rolemberg Andréia Coutinho Facin Julio Edward Hough Monteiro Mareliza Possa de Menezes Maria Eduarda Bastos Andrade Moutinho da Conceição Felipe Augusto Ruiz Sueiro Rosemeri de Oliveira Vasconcelos Paola de Castro Moraes Mirela Tinucci Costa Andrigo Barboza de Nardi 《Topics in companion animal medicine》2018,33(2):35-39
Osteosarcoma is the most commonly reported bone tumor in dogs, typically affecting the axial and appendicular skeleton. Involvement of the paranasal sinuses and the nasal cavity is extremely rare. This report describes a 6-year-old, female, cross-breed dog presented with signs of 90 days duration and diagnosed with a mass located in the right nasal cavity. Radiography, rhinoscopy, and computed tomography findings suggested the presence of an intranasal tumor. Osteosarcoma in the nasal cavity was diagnosed based on histopathological examination and clinical and imaging findings. Survival time was 12 months from diagnosis. 相似文献
72.
Rita Bastos António T. Monteiro Diogo Carvalho Carla Gomes Paulo Travassos João P. Honrado Mário Santos João Alexandre Cabral 《Landscape Ecology》2016,31(4):701-710
Context
Land-use/land-cover (LU/LC) dynamics is one of the main drivers of global environmental change. In the last years, aerial and satellite imagery have been increasingly used to monitor the spatial extent of changes in LU/LC, deriving relevant biophysical parameters (i.e. primary productivity, climate and habitat structure) that have clear implications in determining spatial and temporal patterns of biodiversity, landscape composition and ecosystem services.Objectives
An innovative hierarchical modelling framework was developed in order to address the influence of nested attributes of LU/LC on community-based ecological indicators.Methods
Founded in the principles of the spatially explicit stochastic dynamic methodology (StDM), the proposed methodological advances are supported by the added value of integrating bottom-up interactions between multi-scaled drivers.Results
The dynamics of biophysical multi-attributes of fine-scale subsystem properties are incorporated to inform dynamic patterns at upper hierarchical levels. Since the most relevant trends associated with LU/LC changes are explicitly modelled within the StDM framework, the ecological indicators’ response can be predicted under different social-economic scenarios and site-specific management actions. A demonstrative application is described to illustrate the framework methodological steps, supporting the theoretic principles previously presented.Conclusions
We outline the proposed multi-model framework as a promising tool to integrate relevant biophysical information to support ecosystem management and decision-making.73.
Garcia JL Navarro IT Biazzono L Freire RL da Silva Guimarães Junior J Cryssafidis AL Bugni FM da Cunha IA Hamada FN Dias RC 《Veterinary parasitology》2007,145(3-4):197-206
This study evaluated a vaccine made from crude rhoptry proteins of Toxoplasma gondii with Quil-A, which was administered to cats by the intranasal route. Eleven short-hair domestic cats were divided into four groups: G1 (n=3) received three doses (200 microg/dose) of the rhoptry vaccine with Quil-A (20 microg); G2 (n=3) received PBS with Quil-A (20 microg); G3 (n=3) and G4 (n=2) received only PBS. Treatments were administered at days 0, 21, and 42 by the intranasal route. Challenge was done to G1, G2, and G3 animals with 600 cysts of the VEG strain on day 51 (challenge day); G4 animals were unchallenged. The anti-T. gondii IgG and IgA antibody levels from sera were measured by indirect enzyme-linked immunosorbent assay (ELISA). At challenge, two animals from G1 revealed antibody levels for both IgG and IgA; oocysts were not detected in feces of these two cats. There were no differences in hematological values between groups throughout the experiment (p>0.10). Preventable fractions were 67% in G1 and 0% in G2 and G3. Comparatively, G1 animals shed 89.3% and 90.8% less oocysts than G3 and G4, respectively. Two out of three cats were protected against T. gondii oocyst shedding when the rhoptry vaccine was administered by the intranasal route. This is the first study using crude rhoptry proteins as vaccine by the intranasal route in cats to evaluate protection against oocysts shedding. 相似文献
74.
Isolation and molecular detection of Neospora caninum from naturally infected sheep from Brazil 总被引:1,自引:0,他引:1
Pena HF Soares RM Ragozo AM Monteiro RM Yai LE Nishi SM Gennari SM 《Veterinary parasitology》2007,147(1-2):61-66
Neospora caninum was isolated from a naturally infected sheep from Brazil by bioassay in dogs. Approximately 70g of brain from each of two 4-month-old sheep with indirect fluorescent antibodies (>or=1:50) to N. caninum was offered to a different IFAT negative dog (Sheep n. 302, IFAT 1:400-Dog 1 and Sheep n. 342, IFAT 1:50-Dog 2). Parasite DNA was detected in both sheep brains using a PCR targeting the Nc-5 gene of N. caninum. Shedding of Neospora-like oocysts was noticed only in Dog 1, from 10 days post-inoculation (PI) to 25 days PI (a total of approximately 27,600 oocysts). Seventy days after infection, Dog 1 was euthanized and brain/cerebellum and medulla were collected and submitted to molecular methods, as were the oocysts, to confirm the identity of the isolate. Serum samples collected weekly from both dogs from the infection to the end of the experimental period had no antibodies anti-N. caninum by IFAT (<1:50). Oocysts, brain/cerebellum and medulla specimens of Dog 1 proved positive by a PCR assay targeting the Nc-5 gene of N. caninum. In addition, the oocysts have the DNA amplified by a PCR based on primers directed to the common toxoplasmatiid ITS1 sequence. The PCR products of ITS1 were sequenced, confirming again the isolate as N. caninum. Oocysts were also orally inoculated in two Swiss white mice two Mongolian gerbils (Meriones ungulatus) and two large vesper mice (Calomys callosus) (10(3)oocysts/animal). The rodents were sacrificed 2 months PI, and fresh preparations of brains showed Neospora thick-walled cysts in gerbil brains, but molecular detection using the Nc-5 PCR assay revealed DNA parasite in gerbil and also C. callosus brains. This is the first report of isolation and sequencing of N. caninum from a Brazilian sheep and the first report of molecular detection of N. caninum from C. callosus. 相似文献
75.
Alex P. Justen Antonio R. G. Monteiro Jane M. G. Mikcha Eliane Gasparino Ádina B. Delbem 《Journal Of Aquatic Food Product Technology》2017,26(3):258-266
This study aimed to develop extruded snacks including flour obtained from Nile tilápia carcasses, and then evaluate the chemical composition, colorimetry, thiobarbituric acid reactive substances, and microbiology. There was a significant increase in the levels of crude protein, ether extract, ash, and minerals, as well as a reduction in carbohydrates as more flour was included in the snacks. Increased levels of flavored flour caused reduction in the brightness of snacks. Oxidative rancidity was not detected in snacks. The inclusion of flour improved the nutritional value of extruded snacks without changing sensory characteristics. Microbiologically, the snacks are suitable for human consumption. 相似文献
76.
Rita Constantino Miguel B. Gaspar Fábio Pereira Susana Carvalho João Cúrdia Domitília Matias Carlos C. Monteiro 《水产资源保护:海洋与淡水生态系统》2009,19(5):542-553
- 1. Razor clams are found in different habitats ranging from sheltered systems (e.g. lagoons and estuaries) to open coasts. They are distributed worldwide and comprise a small number of species of high economic value. Depending on the specific habitat and species exploited, different mechanical and hand‐harvesting techniques are employed. While the environmental effects resulting from mechanized methods have been described by several authors, the impacts caused by traditional hand‐harvesting methods remain unknown. Therefore, a study was undertaken in Ria Formosa lagoon (South of Portugal) addressing the environmental effects resulting from harvesting Solen marginatus with salt.
- 2. No significant impact on the sediment was found; the main effect was an increase in salinity after covering the area with salt, which decreased rapidly with the flood tide and after a few hours had returned to pre‐harvesting levels.
- 3. No effects on benthic communities were observed, with similar fluctuation patterns recorded in control and experimental areas, the observed differences being attributed to the natural variability of benthic populations.
- 4. Based on the results obtained in the present study, a razor clam fishery using salt in intertidal areas can be considered environmentally ‘friendly’.
77.
Marta Monteiro Carla Sousa Filipe Coutinho Carolina Castro Filipa Fontinha Inês Guerreiro Pedro Pouso Elisabete Matos Patrícia Díaz-Rosales Aires Oliva-Teles Paula Enes Ana Couto 《Marine drugs》2021,19(11)
Marine algae are recognised sources of bioactive compounds that have attracted great interest as nutritional supplements for aquaculture fish. Intensive rearing conditions often expose fish to husbandry-related stressors, rendering fish more susceptible to disease and reducing production yields. The present work evaluated the potential of two marine algae extracts (Fucus vesiculosus and Nannochloropsis gaditana) as nutritional supplements to mitigate stress effects in meagre (Argyrosomus regius) exposed to an acute handling stress (AS). A plant-based diet was used as a control, and three other diets were prepared, which were similar to the control diet but supplemented with 1% of each algal extract or a combination of the two extracts (0.5% each). The effects of supplemented diets on stress biomarkers, antioxidant enzyme activities, and immune response were analysed in fish exposed to AS after 4 weeks of feeding. Supplemented diets did not affect growth performance but the inclusion of F. vesiculosus promoted higher feed efficiency, as compared to the control group. Dietary algal extracts supplementation reduced plasma glucose levels, increased white blood cell counts, and reduced the expression of pro-inflammatory genes when compared with the control. N. gaditana supplementation led to a reduction in hepatic antioxidant enzyme activity and glutathione levels, while F. vesiculosus supplementation increased muscle glutathione reductase activity and reduced lipid peroxidation. These findings support the potential of algal extracts as nutraceuticals in aquafeeds to enhance the ability of fish to cope with husbandry-related stressful conditions and ultimately improve fish health and welfare. 相似文献
78.
Natalino da Costa Sousa Mrcia Valria Silva do Couto Higo Andrade Abe Peterson Emmanuel Guimares Paixo Carlos Alberto Martins Cordeiro Emilly Monteiro Lopes Jonathan Stuart Ready Gabriel Fernandes Alves Jesus Mauricio Latera Martins Jos Luiz Pereira Mourio Paulo Csar Falanghe Carneiro Alexandre Nizio Maria Rodrigo Yudi Fujimoto 《Aquaculture Research》2019,50(12):3720-3728
Intensive fish farming has resulted in an increased concern for disease outbreaks. Probiotic use is one of the strategies being developed to improve fish health and productivity. Measures of probiotic colonization, growth performance, haematological characteristics and parasite load were used to evaluate the effect of diets supplemented with Enterococcus faecium on growth and health of Arapaima gigas juveniles. A completely randomized design with four treatments (diet with E. faecium at 1 × 106 CFU/g and 1 × 108 CFU/g, control diet and diet with the culture medium MRS) and three replicates was used. Ninety‐six Arapaima juveniles were distributed in 12 cages fed with the specified diet for 68 days. Colonization of the intestinal tract by lactic acid bacteria reduced the total number of heterotrophic bacteria in fish fed with probiotics compared to controls. Fish fed a supplemented diet containing 1 × 108 CFU/g presented higher values of weight gain, survival and fish growth uniformity, and lower values of feed conversion ratio. The prevalence of Trichodina sp. could have affected the survival of fish in the control group. Reduction in parasite load and an increase in haematocrit, the number of erythrocytes, thrombocytes, neutrophils and monocytes were also observed in fish fed the diet containing 1 × 108 CFU/g. Enterococcus faecium presented a probiotic effect in A. gigas juveniles and can be recommended for use at a concentration of 1 × 108 CFU/g to modify the gut microbiota, improve growth performance and haematology and reduce parasitic load. 相似文献
79.
80.
First feeding of diploid and triploid yellowtail tetra Astyanax altiparanae: An initial stage for application in laboratory studies
下载免费PDF全文
![点击此处可从《Aquaculture Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Rafaela Manchin Bertolini José Augusto Senhorini Nivaldo Ferreira do Nascimento Matheus Pereira‐Santos Laura Satiko Okada Nakaghi Wellington Adriano Moreira Peres Regiane Cristina da Silva George Shigueki Yasui 《Aquaculture Research》2018,49(1):68-74
In this study, the aim was to establish a protocol for first feeding of diploid and triploid yellowtail tetra Astyanax altiparanae in laboratory conditions. The fry were fed with five different diets: (i) Artemia franciscana nauplii, (ii) plankton, (iii) dry food, (iv) Artemia franciscana nauplii + plankton, and (v) Artemia nauplii + plankton + dry food. Additionally, the growth and survival rates of diploid and triploid individuals were also evaluated. On day 10, the length of the fish between the treatments differed significantly (p = .0001) and ranged from 4.07 ± 0.06 mm (dry food) to 8.50 ± 0.64 mm (plankton + Artemia). The sizes of the fish increased with time, except for the fish fed with dry food. The survival rates were similar for the fish fed with the four diets and ranged from 80.7 ± 5.4% (dry food + plankton + Artemia to 92.0 ± 1.6% (plankton + Artemia), but differed from the fish fed with dry food (17.7 ± 5.8%, p = .0017). Diploids and triploids did not present differences on day 0 (p = .2252) and on day 10 (p = .4844) when the fish presented 6.77 ± 0.25 mm and 6.54 ± 0.15 mm respectively. Survival of diploids (87.3 ± 5.13%) and triploids (74.67 ± 2.30%) were also similar (p = .0285). These data are innovative and useful for establishing protocols for this species in both academic and applied sciences. 相似文献