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61.
Gordon SG Kowitwanich K Pipatpongpinyo W St Martin SK Dorrance AE 《Phytopathology》2007,97(1):113-118
ABSTRACT Molecular analysis of sources of resistance to plant pathogens should expedite and confirm novel gene discovery and consequently the development of disease resistant cultivars. Recently, soybean plant introductions (PIs) were identified that contain putative novel Rps genes for resistance to Phytophthora sojae. The number of resistance genes that confer resistance to P. sojae isolates OH17 (1b,1d,2,3a,3b,3c,4,5,6,7) and OH25 (1a,1b,1c,1k,7) was then determined in several of the PIs. The objective of this study was to determine if the Rps genes present in these PIs were associated with eight described Rps loci that have been mapped on soybean molecular linkage groups F, G, J, and N. Nine F(2:3) soybean populations were genotyped with simple sequence repeat (SSR) markers linked to previously mapped Rps loci. The nine PI populations all had SSR markers associated (P < 0.01) with resistance to P. sojae isolate OH17 in the Rps1 region. Rps1c is a likely candidate in eight PIs but novel genes may also be possible, while novel genes may confer resistance in one PI to P. sojae isolate OHI7. Two or more Rps genes, including some that are potentially novel, confer resistance to P. sojae isolate OH25 in eight of the populations. However, based on the response to these two isolates, virulence already exists for at least some of the novel genes identified in this study. 相似文献
62.
ABSTRACT Phytophthora sojae, which causes Phytophthora root and stem rot of soybean, is a serious disease worldwide and is managed primarily by deploying cultivars with resistance. Thirty-two soybean plant introductions (PIs), all but three of which were from South Korea, were proposed as new sources of single-gene resistance to P. sojae. The objective of this study was to characterize the inheritance of resistance to P. sojae in these PIs. Twenty-two soybean populations from crosses of these PIs and the susceptible cv. Williams were inoculated with P. sojae OH17 (vir 1b, 1d, 2, 3a, 3b, 3c, 4, 5, 6, 7), and OH25 (vir 1a, 1b, 1c, 1k, 7). These isolates were selected because they are virulent on soybeans with all known Rps genes and many Rps gene combinations. Thirteen of the twenty-two populations had consistent segregation responses following inoculations between the two generations. In two PIs, resistance was conferred by two genes to OH17 and three genes to OH25. Resistance to both isolates was conferred by a single gene in PI 398440 although the individual families were not resistant to the same isolates. The data suggest that six of the populations have three-Rps gene combinations as previously proposed, while another four may have either a novel Rps gene or a four-Rps gene combination. Based on this phenotypic analysis, novel and uncharacterized Rps genes may be present in this material. More importantly, these PIs may serve as sources of novel Rps genes that can be used to more effectively manage Phytophthora root and stem rot. 相似文献
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65.
H. M. Tuihedur Rahman Jiban Chandra Deb Gordon M. Hickey Imrul Kayes 《Journal of Forest Research》2014,19(1):12-21
This research contrasts the financial profitability of different agroforestry practices in community-based buffer zone management of Madhupur National Park (MNP), Bangladesh. This park is the second largest in Bangladesh and subjected to enormous anthropogenic pressure and land rights-related conflicts. This paper reports the different agroforestry practices of villagers in different modules of the buffer zone of MNP, identified by a participatory research approach. It then assesses the profitability of each module on the basis of cost–benefit analysis, net present value, internal rate of return, and annualized income. Results indicate three agroforestry systems were being practised in the buffer zone by the participants: agro-silviculture, agro-silvo-horticulture, and combined woodlot and agroforestry. Among these modules, agro-silvo-horticulture was the most profitable, followed by agro-silviculture then combined woodlot and agroforestry. We recognize that not all of the participants can practise intensive agroforestry; our results suggest that the community-based buffer zone management strategy for MNP would benefit from prioritizing agroforestry practices where possible. This will lead to more successful buffer zone management for the conservation of natural forests while supporting the development of local resource-dependent communities. 相似文献
66.
John Gordon Bell Fiona Strachan Joanne E Good & Douglas R Tocher 《Aquaculture Research》2006,37(6):606-617
Echium oil (EO) is a vegetable oil in which percentages of stearidonic acid (STA, 18:4n‐3) often exceed those of its n‐6 series equivalent γ‐linolenic acid (GLA, 18:3n‐6). Stearidonic acid is elongated to 20:4n‐3 in fish cell cultures, suggesting that EO could be included in diets for marine fish to increase tissue 20:4n‐3 and 20:3n‐6 and, thereby, modulate eicosanoid metabolism. Thus, the present study aimed to test the hypotheses that dietary EO would increase tissue 20:4n‐3 and 20:3n‐6 and modulate immune function and eicosanoid production in juvenile Atlantic cod (Gadus morhua L.) fed a diet where fish oil (FO) was replaced by EO. Duplicate groups of juvenile cod (initial weight ca. 4 g) were fed for 18 weeks on fish meal‐based diets (55% protein and 16% lipid) that differed in oil source (FO or EO). There were no significant differences in growth and feed efficiency, hepato‐somatic index, percentages of liver and flesh lipids and lipid class compositions for cod fed FO and EO. Percentages of 18:4n‐3, 18:3n‐6 and 20:3n‐6 in the total lipids of flesh and liver were higher, and percentages of 20:5n‐3 and 20:4n‐6 were both lower in fish fed EO than in those given FO. In flesh, the increased 18:3n‐6 and 18:4n‐3 were primarily located in phosphatidylcholine and, to a lesser extent, phosphatidylethanolamine, whereas 20:3n‐6 concentration was highest in phosphatidylinositol. Desaturation of 18:3n‐3 (to tetraene products) and 20:5n‐3 to 22:6n‐3 in hepatocytes was very low but was increased by dietary EO. Echium oil significantly decreased the production of prostaglandin F from gill cells stimulated with calcium ionophore A23187, and reduced head kidney macrophage activity, but had no effect on serum lysozyme activity or basic haematology. In conclusion, dietary EO may have beneficial effects on some immune parameters including eicosanoid metabolism in marine fish although this may be primarily because of decreased 20:4n‐6 rather than increasing tissue levels of 20:3n‐6 or 20:4n‐3. 相似文献
67.
Freshly isolated rainbow trout hepatocytes were exposed to tert-butyl hydroperoxide (BuOOH), a substrate for glutathione peroxidase.
BuOOH at a concentration approximately equimolar (1 mM) with intracellular reduced glutathione (GSH) caused a reversible increase
in intracellular glutathione disulphide (GSSG) but did not compromise cell viability or damage membrane lipids. BuOOH at 10
mM caused a large irreversible increase in intracellular GSSG followed by efflux into the medium. Considerable leakage of
lactate dehydrogenase and loss of highly unsaturated fatty acids, particularly docosahexaenoic acid also occurred. Dependence
of hydroperoxide removal on flux through the hexose monophosphate pathway was suggested by the increased release of 14CO2 from [1-14C] glucose from hepatocytes incubated with BuOOH. 相似文献
68.
Hang Ryeol Na Gordon M. Heisler David J. Nowak Richard H. Grant 《Urban Forestry & Urban Greening》2014,13(4):785-792
A mathematical model is constructed for quantifying urban trees’ effects on mitigating the intensity of ultraviolet (UV) radiation on the ground within different land use types across a city. The model is based upon local field data, meteorological data and equations designed to predict the reduced UV fraction due to trees at the ground level. Trees in Seoul, Korea (2010), produced average UV protection factors (UPF) for pedestrians in tree shade at solar noon (May to August) of 8.3 for park and cemetery land uses and 3.0 for commercial and transportation land uses. The highest daily UPF was 11.8 in the park and cemetery land uses, which has the highest percent canopy cover. This UV model is being implemented within the i-Tree modeling system to allow cities across the world to estimate tree effects on UV exposure. Understanding the impacts of urban trees on UV exposure can be used in developing landscape design strategies to help protect urban populations from UV exposure and consequent health impacts. 相似文献
69.
Carbon sequestration potential of five tree species in a 25-year-old temperate tree-based intercropping system in southern Ontario,Canada 总被引:1,自引:0,他引:1
Amy Wotherspoon Naresh V. Thevathasan Andrew M. Gordon R. Paul Voroney 《Agroforestry Systems》2014,88(4):631-643
Carbon (C) sequestration potential was quantified for five tree species, commonly used in tree-based intercropping (TBI) and for conventional agricultural systems in southern Ontario, Canada. In the 25-year-old TBI system, hybrid poplar (Populus deltoides × Populus nigra clone DN-177), Norway spruce (Picae abies), red oak (Quercus rubra), black walnut (Juglans nigra), and white cedar (Thuja occidentalis) were intercropped with soybean (Glycine max). In the conventional agricultural system, soybean was grown as a sole crop. Above- and belowground tree C Content, soil organic C, soil respiration, litterfall and litter decomposition were quantified for each tree species in each system. Total C pools for hybrid poplar, white cedar, red oak, black walnut, Norway spruce and a soybean sole-cropping system were 113.4, 99.4, 99.2, 91.5, 91.3, and 71.1 t C ha?1, respectively at a tree density of 111 trees ha?1, including mean tree C content and soil organic C stocks. Net C flux for hybrid poplar, white cedar, red oak, black walnut, Norway spruce and soybean sole-crop were 2.1, 1.4, 0.8, 1.8, 1.6 and ?1.2 t C ha?1 year?1, respectively. Results presented suggest greater atmospheric CO2 sequestration potential for all five tree species when compared to a conventional agricultural system. 相似文献
70.
Estimating coarse root biomass with ground penetrating radar in a tree-based intercropping system 总被引:1,自引:0,他引:1
Kira A. Borden Marney E. Isaac Naresh V. Thevathasan Andrew M. Gordon Sean C. Thomas 《Agroforestry Systems》2014,88(4):657-669
Conventional measurements of tree root biomass in tree-based intercropping (TBI) systems can be inadequate in capturing the heterogeneity of rooting patterns or can be highly destructive and non-repeatable. In this study, we estimated coarse root biomass using ground penetrating radar (GPR) of 25-year-old trees inclusive of five species (Populus deltoides × nigra clone DN-177, Juglans nigra L., Quercus rubra L., Picea abies L. Karst, and Thuja occidentalis L.) at a TBI site in Southern Ontario, Canada. Subsurface images generated by GPR were collected in grids (4.5 × 4.5 m) centred on tree stems. The predictive relationship developed between GPR signal response and root biomass was corrected for species effects prior to tree-scale estimates of belowground biomass. Accuracy of the tree-scale estimates was assessed by comparing coarse root biomass measured from complete excavations of the corresponding tree. The mean coarse root biomass estimated from GPR analysis was 54.1 ± 8.7 kg tree?1 (mean ± S.E.; n = 12), within 1 % of the mean coarse root biomass measured from excavation. Overall there was a root mean square error of 14.4 kg between measured and estimated biomass with no detectable bias despite variable conditions within the in-field and multi-species study. Root system C storage by species, calculated with species-specific root carbon concentrations, is estimated at 5.4 ± 0.7–34.8 ± 6.9 kg C tree?1 at this site. GPR is an effective tool for non-destructively predicting coarse root biomass in multi-species environments such as temperate TBI systems. 相似文献