云南引进帝国百脉根的研究

对引进帝国百脉根进行茎叶比、干鲜比、干草产量、营养成分和体外干物质消化率(IVDMD)的测定。结果表明，帝国百脉根含有丰富的营养，分枝期和盛花期粗蛋白含量分别高达28．0%和20．79%，粗纤维含量为13．0%和17．46%，Ca、P 丰富，比例恰当，体外干物质消化率较高而稳定，在分枝期和开花期分别为58．64%和57．25%。因此，帝国百脉根为饲用价值较高的优良豆科牧草，应予以进一步开发利用。     

黄金茶CsDAM2基因克隆及其功能分析

休眠是通过植物体内相应基因的表达调控机制,使植物芽停止生长,以适应外界低温、短日照等不良环境条件的结果。茶芽打破休眠的早晚,密切关系到茶树经济生产的效益。为进一步掌握茶树芽休眠与解除的分子机理>本研究克隆了可能与黄金茶发芽早相关的转录因子(dormancy associated MADS-box CsDAM2),并在烟草中过表达CsDAM2,对CsDAM2转基因烟草和野生型烟草进行低温处理。结果表明,在相同处理条件下,随着温度的降低,同野生型烟草比较,转基因烟草电导率增加幅度小,并且叶绿素荧光值降低率低,说明在抗寒性能上,转基因烟草具有显著优势,进而一定程度上证实了 CsDAM2具有提髙茶树抗寒性的作用。本研究结果为进一步揭示茶树芽休眠与解除的分子机理提供了科学依据。     

基于屈服强度的X80钢管100％SMYS试压的可行性

高强度试压是国内外管道试压的普遍趋势.为研究我国X80钢管100％SMYS(最小屈服强度)试压的可行性,以西气东输二线西段为例,应用概率统计方法,分析了X80直缝钢管和螺旋缝钢管管材试样屈服强度的分布特征,并研究了实物屈服强度和管材试样屈服强度的差异.结果表明:X80直缝钢管和螺旋缝钢管试样的屈服强度分布均符合正态分布,X80直缝钢管屈服强度性能优于螺旋缝钢管,钢管实物屈服强度比管材小试样屈服强度高10％～15％.以100％SMYS试压导致母材屈服概率小于1％为目标,根据管材试样屈服强度概率分布特征及试样与实物屈服强度的差异,得出X80钢管可进行100％SMYS试压的结论.     

Impacts of diet on hindgut microbiota and short‐chain fatty acids in grass carp (Ctenopharyngodon idellus)

Diet is known to influence intestinal microbiota in fish, but the specifics of these impacts are still poorly understood. Different protein/fibre ratio diets may result in differing structures and activities of gut microbiota. We examined the hindgut microbiome of grass carp (Ctenopharyngodon idellus) fed three different diets: fish meal (FM, high protein – low fibre), Sudan grass (SG, high fibre – low protein) and compound feed (CF, intermediate). Microbial profiles of fish fed on FM were significantly different from profiles of fish fed CF and SG (F = 18.85, p < .01). Cetobacterium, known to be positively associated with protein digestion, was the dominant microbial group in FM samples (approximately 75.7%), while Lachnospiraceae and Erysipelotrichaceae, thought to be involved in fermentation of plant polysaccharides, were dominant in CF and SG samples (46.8% and 42.9% respectively). Network analyses indicated that the abundance of Lachnospiraceae and Erysipelotrichaceae was in a significantly positive correlation (r = .895, p = .001). Short‐chain fatty acid (SCFA) levels may indicate that the digestibility of diet by microbiota in the grass carp gut decreased from FM to SG (FM>CF>SG). Overall low SCFA levels indicate that hindgut fermentation probably provides a low proportion of energy requirements in grass carp.     

美洲鲥致病性维氏气单胞菌的分离鉴定及药敏试验

从自然感染发病的美洲鲥肝脏、肾脏和肠道中分离出1株细菌,经革兰染色镜检及PCR鉴定,确定为维氏气单胞菌(Aeromonas veronii)。用该菌株接种健康美洲鲥,鉴定其致病力,从人工感染50 g左右的健康美洲鲥发现,菌株具有很强的致病力,试验鱼在感染后72 h内全部死亡,且患病症状与自然发病的鱼症状一致。进一步采用药敏纸片法测定分离菌株对多种抗生素的敏感性,结果发现:维氏气单胞菌对恩诺沙星、氧氟沙星、头孢噻吩三种药物极为敏感,对红霉素、头孢唑啉中度敏感,对青霉素、氨苄西林、阿莫西林、四环素、链霉素不敏感。     

Digestibility of the defatted microalgae Nannochloropsis sp. and Desmodesmus sp. when fed to Atlantic salmon,Salmo salar

The aim of the study was to investigate the apparent digestibility coefficient (ADC) of defatted biomass derived from microalgae Nannochloropsis sp. and Desmodesmus sp. when fed to Atlantic salmon postsmolts in seawater. Two experiments, one employing cold‐pelleted and the other employing extruded pellets, were carried out to determine the ADC of dry matter (DM), protein, ash and energy. The test feeds consisted of a fishmeal‐based reference feed and the algal biomass, at a ratio of 70:30, and yttrium oxide as the inert marker. The ADC of DM and protein in fish fed microalgae‐incorporated cold‐pelleted feeds were significantly higher for Nannochloropsis sp. compared with Desmodesmus sp. The Nannochloropsis sp. inclusion in extruded feeds also led to higher ADCs of DM and energy compared with Desmodesmus sp. The extrusion processing significantly increased the ADC of ash in both defatted microalgae biomass, as well as ADCs of DM in Nannochloropsis and protein in Desmodesmus sp. In conclusion, the microalga Nannochloropsis sp. was more digestible than Desmodesmus sp., and extrusion processing can be used as a means to improve digestibility of certain nutrients.     

几个杂交稻组合的适应性分析

以汕优63为对照,通过比较Ⅱ优084、Ⅱ优838、Ⅱ优725、粤优938、两优培九和丰优香占等6个组合对水稻主要病害的抗性、秧龄弹性以及对高温和涝害适应的能力,研究结果表明:6个组合中Ⅱ优084适应性最好,总体上强于汕优63;其次为Ⅱ优838和Ⅱ优725,总体上与汕优63相当;粤优938、两优培九和丰优香占的适应性总体上不如汕优63。     

饲料加工中水分在线控制系统研究进展

水分在线控制系统可实时准确检测饲料的水分,并能保证使饲料的水分在国家规定的范围内,且能满足用户的要求。主要的方法是在待制粒仓内检测待制粒料的实时水分,在冷却器排料口检测成品饲料的实时水分;待制粒料的水分是为调质器加水作依据,而成品水分是保证饲料品质的依据。成品水分偏高,可通过控制冷却器风量来降低,水分偏低,又为调质器加水作参考。本系统通过在几个饲料厂的实际应用,证明完全符合设计要求。     

探析IPv6技术

阐述了IPv4现存在的一些问题和IPv6的一些优点,在此基础上对IPv6、DNS进行简单的介绍。