The influence of diet on the grass carp intestinal microbiota and bile acids

To elucidate the influence of different diet on the intestinal microbe and bile acids, we characterized the microbiota and bile acids in the hindgut content of grass carp fed on formula feed (FF group) or Sudan grass (SG group). Fusobacteria and Proteobacteria were significantly more represented in FF group than in SG group whereas Bacteroidetes was significantly more abundant in SG group than in FF group. Simpson diversity was significantly higher in FF group than in SG group (t = 2.33, P < 0.05). Chenodeoxycholic acid (CDCA) was the most abundant primary bile acid in the two groups, with average concentrations of 1.03 ± 0.62 and 4.44 ± 1.80 ng mg^?1 in SG and FF group respectively. The most abundant secondary bile acid was deoxycholic acid (DCA) in SG group and ursodeoxycholic acid (UDCA) in FF group, with average concentrations of 0.17 ± 0.06 and 2.67 ± 0.88 ng mg^?1 respectively. UDCA is significantly more abundant in FF group than in SG group, and the total bile acids were higher in FF group than in SG group. Cetobacterium and Fusobacteriaceae U114 were significantly related with the concentrations of CDCA (r = 0.85, P < 0.05 and r = 0.82, P < 0.05 respectively) and UDCA (r = 0.92, P < 0.01 and r = 0.92, P < 0.01 respectively). However, Bacteroides was negatively related with the concentration of UDCA (r = ?0.67, P < 0.05). Overall, there existed certain relationship between the intestinal microbes and the faecal bile acids, and they were both influenced by the diet.     

Two biomass preparation methods provide insights into studying microbial communities of intestinal mucosa in grass carp (Ctenopharyngodon idellus)

Animal digestive tract is habitat for a large number of autochthonous microbiota, which play central roles in multiple biological and physiological processes of the host. In this study, two different micro‐biomass preparation methods were employed to evaluate the diversity of intestinal mucosa‐associated microbiota in grass carp (Ctenopharyngodon idellus). Genomic DNAs were isolated either directly from intestinal mucosal samples (group A), or from micro‐biomass after microbial dissociation (group B). Community richness, diversity and evenness indices were all higher in group B, but differences were not statistically significant (P = 0.97, P = 0.33, P = 0.34 respectively). Furthermore, group B samples exhibited an increased ratio of bacterial DNA in comparison with group A samples, but the difference was also not statistically significant (P = 0.74). In addition, there were no statistically significant differences between the two groups (P > 0.05) at the taxonomic level. Our results support previous findings that there exists a great abundance of the intestinal mucosa‐adherent microbiota in the grass carp; among these, Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Spirochaetes and Fusobacteria were the most common phyla. Within these microbiota, Paenibacillus, Bacteroides, Bacillus and Cetobacterium genera comprise the majority of the community, implicating their functional importance (e.g. as probiotics) to their host. Our results contribute towards a better understanding of the intestinal microbial profile of grass carp. Both micro‐biomass preparation techniques proved to be feasible for studying mucosa‐adherent microbiota of grass carp; however, the second method (group B) provides a protocol that is somewhat more effective than the first method (group A).     

Reproductive resilience: a paradigm shift in understanding spawner‐recruit systems in exploited marine fish

A close relationship between adult abundance and stock productivity may not exist for many marine fish stocks, resulting in concern that the management goal of maximum sustainable yield is either inefficient or risky. Although reproductive success is tightly coupled with adult abundance and fecundity in many terrestrial animals, in exploited marine fish where and when fish spawn and consequent dispersal dynamics may have a greater impact. Here, we propose an eco‐evolutionary perspective, reproductive resilience, to understand connectivity and productivity in marine fish. Reproductive resilience is the capacity of a population to maintain the reproductive success needed to result in long‐term population stability despite disturbances. A stock's reproductive resilience is driven by the underlying traits in its spawner‐recruit system, selected for over evolutionary timescales, and the ecological context within which it is operating. Spawner‐recruit systems are species specific, have both density‐dependent and fitness feedback loops and are made up of fixed, behavioural and ecologically variable traits. They operate over multiple temporal, spatial and biological scales, with trait diversity affecting reproductive resilience at both the population and individual (i.e. portfolio) scales. Models of spawner‐recruit systems fall within three categories: (i) two‐dimensional models (i.e. spawner and recruit); (ii) process‐based biophysical dispersal models which integrate physical and environmental processes into understanding recruitment; and (iii) complex spatially explicit integrated life cycle models. We review these models and their underlying assumptions about reproductive success vs. our emerging mechanistic understanding. We conclude with practical guidelines for integrating reproductive resilience into assessments of population connectivity and stock productivity.     

Phosphorus‐enriched soils of urban and suburban Nanjing and their effect on groundwater phosphorus

Neither the phosphorus (P)‐rich soils in urban areas nor their environmental implications have been adequately studied. This study investigated soils of typical urban function zones in Nanjing/China, like park, residential areas, school yards, campus as well as suburb vegetable land and garbage filling sites, and meantime ground water in situ. Typical soils were also experimentally leached for P leaching evaluation. All studied soils were enriched with P with enrichment ratios varying from 2 to 10 for total P and 5 to 22 for NaHCO₃‐extractable P, as compared with the original parent soils. The C : P ratios also indicated strong enrichment of P in urban soils. In urban areas the maximum P layer appeared as buried under different depth while in suburban soils as epipedon. The various morphology of P distribution suggested different soil formation patterns, which were related to the land use history. Groundwater P was significantly correlated with the maximum extractable P content of P in soil profiles and even better with the weighted average P content of the whole profile or P content of the soil layer at or close to groundwater table. Dissolved P in experimental leachate was comparable with that of groundwater but higher than environmentally acceptable level. Furthermore, there was a significant correlation between solution P and different extractable P forms of the studied soils. Simple P tests can provide an evaluation of the potential risk of urban soils in discharging P to water system.     

Soil Quality Indicators in Relation to Land Use and Topography in a Small Catchment on the Loess Plateau of China

Better understanding of how the loess soils respond to topography and land use under catchment‐scale vegetation restoration is needed to enable science‐based land management interventions for the policy‐driven “Grain‐for‐Green” eco‐restoration program in the Loess Plateau of China. The objective of this study was to characterize the relationships of four selected soil quality indicators to land use under vegetation restoration and topography for a small catchment (0·58 km²) in the Loess Plateau. The major land uses established in the catchment are cropland, fallow (i.e., natural revegetation), grassland, and jujube orchard. The four soil quality indicators were soil organic carbon (SOC), soil total nitrogen (STN), soil total phosphorus (STP), and mean root zone soil water content during the wet season (MRZSWwet). SOC, STN, and MRZSWwet were significantly different (p < 0·05) for different land uses. Grassland showed the highest values for these three properties, whereas cropland had relatively low values for SOC and STN. Land use had no effect on STP, although the lowest value was observed in grassland. Spatial analysis showed that various relations between soil quality indicators and topography (slope and elevation) were observed. These relations were generally weak for most of them, and they varied with land uses. Further analyses indicated that land uses, slope, and elevation had significant effects on the relations between different soil quality indicators. The results here should provide useful information for the further development of “Grain‐for‐Green” program. Copyright © 2012 John Wiley & Sons, Ltd.     

温室环境多级控制系统及优化目标值设定的初步研究

该文采用多级控制策略,优化设定系统目标值来解决温室环境系统中多个时间响应常数相差过大的问题;设定系统优化目标值时,白天使植物获得最大的光合速率,夜间在满足植物生长和积温要求的前提下使温室处在能耗最小的状态下运行。构建了能量消耗为零（无加热、无制冷和无机械通风）时计算温室内部温度的模型,采用遗传算法对最优目标值进行搜索。计算结果表明：该系统优于某地先进的温室控制系统,既能获得较高的效率又能节能。     

基于非稳态热探针法的稻谷导热系数的测定

根据传热理论,导出探针法测定稻谷导热系数的数学模型。通过测定探针的升温速率,由上述数学模型计算出不同含水率稻谷的导热系数。实验结果表明,在常温下,产于我国南方地区的短粒型稻谷的导热系数随含水率的增加而增大。回归分析表明,短粒型稻谷的导热系数与含水率之间为线性关系。     

基于圆筒瞬态热流法的短粒型稻谷导温系数的测定

根据圆筒瞬态热流法的理论模型 ,利用自制的导温系数测定仪 ,对产于我国南方地区的短粒型稻谷的导温系数进行了测定 ,并研究了含水率对稻谷导温系数的影响。结果显示 ,在 2 0～ 80℃的温度范围内 ,对于含水率在 6 .4 8%～ 19.8%的稻谷 ,其导温系数与含水率之间不具有线性相关性。置信度为 95 %时 ,短粒型稻谷的导温系数为 (0 .14 6± 0 .0 0 3)μm2 / s。     

喷头喷洒雨滴粒径测试的改进研究

色斑法是一种应用广泛的测试雨滴粒径大小的方法。但以往的雨滴率定只考虑色斑直径与雨滴真实粒径的关系,忽略了高度对率定的影响。在大量试验的基础上对数据进行多元回归分析,把高度因素作为影响雨滴粒径测量公式中一个因子。通过数据处理,得到关于高度和色斑直径双因子共同作用下的雨滴粒径的率定公式。并在处理数据过程中对公式的相关性进行了分析,经过与试验数据的比较,发现公式的拟合性好。     

Integrating remote sensing, census and weather data for an assessment of rice yield, water consumption and water productivity in the Indo-Gangetic river basin

Crop consumptive water use and productivity are key elements to understand basin water management performance. This article presents a simplified approach to map rice (Oryza sativa L.) water consumption, yield, and water productivity (WP) in the Indo-Gangetic Basin (IGB) by combining remotely sensed imagery, national census and meteorological data. The statistical rice cropped area and production data were synthesized to calculate district-level land productivity, which is then further extrapolated to pixel-level values using MODIS NDVI product based on a crop dominance map. The water consumption by actual evapotranspiration is estimated with Simplified Surface Energy Balance (SSEB) model taking meteorological data and MODIS land surface temperature products as inputs. WP maps are then generated by dividing the rice productivity map with the seasonal actual evapotranspiration (ET) map. The average rice yields for Pakistan, India, Nepal and Bangladesh in the basin are 2.60, 2.53, 3.54 and 2.75 tons/ha, respectively. The average rice ET is 416 mm, accounting for only 68.2% of potential ET. The average WP of rice is 0.74 kg/m³. The WP generally varies with the trends of yield variation. A comparative analysis of ET, yield, rainfall and WP maps indicates greater scope for improvement of the downstream areas of the Ganges basin. The method proposed is simple, with satisfactory accuracy, and can be easily applied elsewhere.