全文获取类型
收费全文 | 119篇 |
免费 | 6篇 |
专业分类
林业 | 1篇 |
3篇 | |
水产渔业 | 3篇 |
畜牧兽医 | 116篇 |
植物保护 | 2篇 |
出版年
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 4篇 |
2014年 | 3篇 |
2013年 | 11篇 |
2012年 | 2篇 |
2011年 | 7篇 |
2010年 | 5篇 |
2009年 | 7篇 |
2008年 | 7篇 |
2007年 | 12篇 |
2006年 | 4篇 |
2005年 | 11篇 |
2004年 | 6篇 |
2003年 | 9篇 |
2002年 | 8篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 6篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有125条查询结果,搜索用时 15 毫秒
51.
Tiels P Verdonck F Coddens A Ameloot P Goddeeris B Cox E 《Veterinary microbiology》2007,119(2-4):115-120
F18+ Escherichia coli can cause post-weaning diarrhoea and oedema disease in pigs. These diseases are responsible for substantial economic losses, but a vaccine is not available. A good knowledge of the characteristic of the fimbriae is useful for the development of a vaccine composed of the fimbrial virulence factor. F18 fimbriae are composed of the major subunit FedA and the minor subunits FedE and the adhesin FedF. In the present study monoclonal antibodies (mAbs) against FedA and FedF were produced. In addition to their diagnostic value, these mAbs revealed a weaker interaction between FedA and FedF compared to the subunit-subunit interactions in other fimbriae, like type 1 and P pili. Further experiments are needed to investigate if this weak interaction could be one of the reasons for the slow colonisation of the small intestinal mucosa by F18+ E. coli. 相似文献
52.
In order to limit the number and impact of exotic pest invasions, leading-edge technologies must be embraced and embedded within integrated national and international biosecurity systems. Outlined here are recent advances in the detection of exotic pests, and prospects for the early recognition of disease. Applications of new tools are described, using our understanding of the genomes of pathogens and vectors. In addition, the role of mathematical and simulation models to aid both biosecurity planning, and decision making in the face of an epidemic, are discussed, and recent attempts to unify epidemiology and evolutionary dynamics are outlined. Given the importance of emerging diseases and zoonoses, the need to align human and veterinary surveillance within fully integrated systems is underlined. 相似文献
53.
Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection
54.
Consumer interest in social sustainability issues of whitefish from capture fisheries in the north‐east Atlantic 下载免费PDF全文
Linda J L Veldhuizen Ivo A van der Lans Paul BM Berentsen Imke J M de Boer Eddie A M Bokkers 《Fish and Fisheries》2017,18(3):527-542
Capture fisheries in the north‐east Atlantic account for approximately 10% of all fish consumed from capture fisheries globally. The literature shows that consumers show considerable interest in social sustainability of products in general and of fish specifically. This interest, however, has not yet been investigated for fish from the north‐east Atlantic. The first objective of this study, therefore, was to investigate whether consumers are interested in social sustainability issues of whitefish from the north‐east Atlantic by determining preferences for four social sustainability issues with distinct benefits in relation to a known reference point (i.e. approach to overfishing). The second objective of this study was to determine to what degree case‐specific and general psychographic consumer characteristics explain preferences for these issues. Choice modelling results from an online survey among 457 Dutch consumers show that consumers have the strongest preference for the environmental sustainability issue approach to overfishing. In addition, results on the social sustainability issues showed that consumers prefer fish welfare over product quality, worker safety and local employment, indicating that in this case, consumers place animal benefits over personal, worker and community benefits. The case‐specific psychographic characteristic concern contributed most to explaining preferences for the environmental sustainability issue, whereas the general psychographic characteristic personal relevance contributed most to explaining preferences for the social sustainability issues. This result is likely explained by the principal focus on MSC certification in markets for fish products, which caused consumers to form opinions (e.g. concern) on overfishing, but not on social sustainability. 相似文献
55.
Decreased fertility in pigs is a common occurrence during summer months. An objective of the current experiments was to evaluate if elevated ambient temperature altered the oocyte plasma membrane including potential receptors for sperm. This would potentially contribute to reduced fertilizability. Treated gilts were exposed in vivo to 32°C for 12 h per day and 20°C for the remaining 12 h per day for 7 days; control gilts were exposed to 22°C for 12 h and 20°C for the remaining 12 h each day. Cumulus–oocyte complexes were also aspirated from ovaries obtained from gilts maintained at thermoneutral ambient temperature and matured in vitro at 38.5°C or 40°C. Relative abundance of a porcine oocyte membrane protein was examined by intensity of immunolabelling of the in vivo and in vitro matured oocytes evaluated with confocal microscopy; fertilizability of the in vitro matured oocytes was evaluated in in vitro fertilization assays. Oocytes obtained from gilts exposed to elevated ambient temperature for 7 days had reduced immunolabelling compared with oocytes from control gilts (p < 0.05). Similarly, oocytes matured in vitro for 44 h at elevated ambient temperature had reduced immunolabelling and reduced fertilizability compared with oocytes matured at 38.5°C (p < 0.01 and p < 0.05). These results suggest porcine oocyte quality is reduced by elevated ambient temperature and immunolabelling of oocytes with antibodies to specific membrane proteins may be effective to evaluate some aspects of oocyte quality. 相似文献
56.
Holland WG Claes F My LN Thanh NG Tam PT Verloo D Büscher P Goddeeris B Vercruysse J 《Veterinary parasitology》2001,97(1):23-33
In this study five parasitological methods and a polymerase chain reaction (PCR) were compared for the diagnostic sensitivity for Trypanosoma evansi in experimentally infected water buffaloes over a period of 15 weeks. The combined estimates of sensitivity (CE(se)) of the PCR proved to be highest at 78.2%, closely followed by the mouse inoculation (MI), the micro-haematocrite centrifugation technique (MHCT) and the mini-anion-exchange centrifugation technique (MAECT) with CE(se) of, respectively, 74.0, 69.6 and 62.4%. The CE(se) of the buffy-coat technique (BCT) at 38.6% and the sodium dodecyl sulfate (SDS) clarification technique at 25.1% were considerably lower. PCR detected consistently all buffaloes infected from week 3 post-infection (PI) onwards. For MI this occurred after 5 weeks PI while for MHCT and MAECT these sustainable high levels were reached in the 7th week PI. BCT and SDS never detected all buffaloes infected. The influence of time and temperature on the viability of T. evansi in heparinized blood from water buffalo was also studied. In general we observed that the survival time tends to be longer when blood is kept at 4 degrees C. In samples kept in direct sunlight parasites became undetectable with the MHCT after 30min. After treatment of the water buffaloes with diminazene aceturate, the PCR signal disappeared within 24h. 相似文献
57.
Holland WG Thanh NG Do TT Sangmaneedet S Goddeeris B Vercruysse J 《Tropical animal health and production》2005,37(6):457-467
This study is concerned with the evaluation of established diagnostic tests for diagnosis of Trypanosoma evansi in pigs. The immune trypanolysis test (TL), card agglutination test (CATT), latex agglutination test (LATEX), enzyme-linked
immunosorbent assay (ELISA), microhaematocrit centrifugation technique (MHCT) and mouse inoculation (MI) tests were initially
evaluated in experimentally infected fattening pigs. All infected pigs were confirmed parasitologically positive with both
MHCT and MI. Results of the serological assays indicated that the TL could be a reference test for the presence of RoTat 1.2
antibodies in pigs. The results of the CATT and LATEX were inconsistent with the TL while the ELISA results correlated with
the TL results. The four serological assays were subsequently used in two field surveys in Vietnam and Thailand. Results of
the two agglutination assays (CATT and LATEX) were not consistent and did not correlate with TL results. The ELISA at percentage
positivity of 22 appeared to have good ability to discriminate between seropositive and seronegative animals. Of the 437 samples
collected at smallholder pig premises in northern Vietnam, no positive pigs were detected with the TL test. In Thailand, 77
samples were collected from five farrowing farms with a history of surra. Two parasitologically positive sows were found and
on each farm seropositive sows were detected. 相似文献
58.
Claes F Ilgekbayeva GD Verloo D Saidouldin TS Geerts S Buscher P Goddeeris BM 《Veterinary parasitology》2005,131(3-4):221-225
In this study, we compared the complement fixation test (CFT), the horse complement fixation test (HCFT) and a card agglutination test for trypanosomosis (CATT/T. evansi) for the diagnosis of equine trypanosomosis in the Republic of Kazakhstan. Cohen's kappa test was used to evaluate the concordance between the three tests. Kappa scores for CFT versus HCFT and CATT are both 0.6165 (95% Confidence Interval CI 0.414--0.819) indicating a "substantial" agreement between CFT and HCFT or CATT, respectively. Kappa for HCFT versus CATT is 0.395 (CI 0.142--0.648) indicating a "fair" agreement between the two tests. In the absence of a golden standard, seroprevalence and sensitivity and specificity of the three tests were estimated using maximum likelihood estimation. CFT has a sensitivity of 57.2% (CI 31.5--79.5%) and a specificity of 95.8% (CI 89.2--98.5%), HCFT has a sensitivity of 80.6% (CI 44.1--95.6%) and a specificity of 99.5% (CI 90.7--100%), CATT has a sensitivity of 80.2% (CI 44.5--95.2%) and a specificity of 98.5% (CI 79.5--99.9%). The seroprevalence of equine trypanosomosis in Kazakhstan was estimated at 16.4% (CI 9.4--27.0%). The data suggest that for epidemiological studies and the control of equine trypanosomosis serological tests prove useful since they have a high specificity and a satisfactory sensitivity. Field applicable tests, such as CATT/T. evansi may be used to replace laboratory-based tests, such as CFT and HCFT. 相似文献
59.
60.
Holland WG Thanh NG My LN Do TT Goddeeris BM Vercruysse J 《Tropical animal health and production》2004,36(1):45-48
Tropical Animal Health and Production - 相似文献