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171.
172.
Identification of a universal Group B streptococcus vaccine by multiple genome screen 总被引:2,自引:0,他引:2
Maione D Margarit I Rinaudo CD Masignani V Mora M Scarselli M Tettelin H Brettoni C Iacobini ET Rosini R D'Agostino N Miorin L Buccato S Mariani M Galli G Nogarotto R Nardi-Dei V Nardi Dei V Vegni F Fraser C Mancuso G Teti G Madoff LC Paoletti LC Rappuoli R Kasper DL Telford JL Grandi G 《Science (New York, N.Y.)》2005,309(5731):148-150
Group B Streptococcus (GBS) is a multiserotype bacterial pathogen representing a major cause of life-threatening infections in newborns. To develop a broadly protective vaccine, we analyzed the genome sequences of eight GBS isolates and cloned and tested 312 surface proteins as vaccines. Four proteins elicited protection in mice, and their combination proved highly protective against a large panel of strains, including all circulating serotypes. Protection also correlated with antigen accessibility on the bacterial surface and with the induction of opsonophagocytic antibodies. Multigenome analysis and screening described here represent a powerful strategy for identifying potential vaccine candidates against highly variable pathogens. 相似文献
173.
Cell therapy of alpha-sarcoglycan null dystrophic mice through intra-arterial delivery of mesoangioblasts 总被引:1,自引:0,他引:1
Sampaolesi M Torrente Y Innocenzi A Tonlorenzi R D'Antona G Pellegrino MA Barresi R Bresolin N De Angelis MG Campbell KP Bottinelli R Cossu G 《Science (New York, N.Y.)》2003,301(5632):487-492
Preclinical or clinical trials for muscular dystrophies have met with modest success, mainly because of inefficient delivery of viral vectors or donor cells to dystrophic muscles. We report here that intra-arterial delivery of wild-type mesoangioblasts, a class of vessel-associated stem cells, corrects morphologically and functionally the dystrophic phenotype of virtually all downstream muscles in adult immunocompetent alpha-sarcoglycan (alpha-SG) null mice, a model organism for limb-girdle muscular dystrophy. When mesoangioblasts isolated from juvenile dystrophic mice and transduced with a lentiviral vector expressing alpha-SG were injected into the femoral artery of dystrophic mice, they reconstituted skeletal muscle in a manner similar to that seen in wild-type cells. The success of this protocol was mainly due to widespread distribution of donor stem cells through the capillary network, a distinct advantage of this strategy over previous approaches. 相似文献
174.
Nadia Musco Valeria Maria Morittu Vincenzo Mastellone Anna Antonella Spina Giuseppe Vassalotti Biagio D’Aniello Raffaella Tudisco Federico Infascelli Pietro Lombardi 《Journal of animal physiology and animal nutrition》2021,105(Z1):26-33
A nutritional supplement (Ecotrofin™, by Vetoquinol Italia S.r.l) recommended in ruminants feeding to strengthen the physiological condition and improve digestive performance was tested in 20 pluriparae grazing goats divided in two groups (control and treated) to assess its possible effects on milk yield and quality and to assess eventual adverse effects. Animals from both groups also received 400 g/day of corn meal, and the treated group was supplemented with 20 g/head/day of the nutritional supplement. At the doses suggested by the manufacturer, despite a transient increase after 30 days of supplementation, Ecotrofin™ did not show significant effects on milk yield and, although some changes were found in the fatty acids profile, no significant improvement of MUFA and PUFA, as well as of omega-6:omega-3 ratio and CLA content were seen. Therefore, in our experimental conditions the supplementation of diet with Ecotrofin™ did not appear useful to improve goat's performance. A significant effect on kidney health markers (27 vs. 22.5 for urea and 0.83 vs. 0.76 for creatinine, p < 0.05) suggested a beneficial effect on renal function but, since levels fell in the normal ranges in both groups, such hypothesis would need further studies to be addressed. 相似文献
175.
Alessandro Vetere Mara Bertocchi Igor Pelizzone Giuseppe F. La Cauza Laura Denti Enrico Bigliardi Francesco Di Ianni 《Journal of veterinary diagnostic investigation》2021,33(1):116
Forty-five Horsfield’s tortoises (Testudo horsfieldii; syn. Agrionemys horfieldii, Russian tortoise) belonging to different owners had decreased appetite and respiratory issues. Twenty-nine tortoises had epiphora, dyspnea, and white necrotic diphtheroid oral plaques (group G1). Ten of the remaining 16 tortoises had serious dehydration, appetite disorder, and depression (G2). The last 6 tortoises had only decreased appetite and moderate conjunctival discharge (G3). During the physical examination of all 45 tortoises, a cytologic sample and an oral swab for herpesvirus and Mycoplasma agassizii PCR testing were taken. In 20 of 29 specimens from G1, in 8 of 16 from G2, and 0 of 6 from G3, the cytologic exam revealed intranuclear acidophilic inclusion bodies, multinucleate cellular syncytia, and further abnormalities caused by herpesviral infection. Moreover, all 45 tested subjects were found to be positive for testudinid herpesvirus 1; 2 were positive for M. agassizii. This prospective study suggests that Horsfield’s tortoises with such signs would benefit from this screening procedure, given that it was effective in a significant proportion of infected and symptomatic animals, and no negative effects were seen. 相似文献
176.
Maria Caria Carlo Boselli Angela Calvo Silverio Grande Giuseppe Todde 《Animal Science Journal》2021,92(1):e13667
The objectives of this study were to evaluate and compare the effect of working vacuum levels (35 and 44 kPa) and liners dimensions (mouthpiece lip diameter and overall length, 20–185 and 22–170 mm) on the main milking characteristics of goats. The results highlight that both the working vacuum level and the liner dimension have influenced the milk flow curve parameters. The maximum variations were found for peak flow rate, which increased significantly with liner dimensions of 20–185 mm at a working vacuum level of 44 kPa as well as average milk flow rate and for plateau phase duration. The incorrect adoption of operative parameters and unsuitable milking machine components, might affect the performance of the mechanical milking and negatively affecting animal productions and welfare. 相似文献
177.
Dario Di Giuseppe Massimiliano Melchiorre Umberto Tessari Barbara Faccini 《Journal of Soils and Sediments》2016,16(3):909-915
Purpose
An analytical database containing XRF chemical analyses and real density measurements of unconsolidated sediments of the Padania Plain (Northern Italy) has been used to understand the relationship that exists between the soil particle density (ρs) and their bulk chemical composition.Materials and methods
Using a linear regression, we built an equation able to link the particle density with the soil elemental composition.Results and discussion
Positive correlations were found between ρs and SiO2, MgO, CaO and Na2O and negative correlations with K2O, TiO2, Al2O3, Fe2O3 and LOI, reflecting the presence in the soils of quartz and feldspars/mineral clays respectively.Conclusions
Our equation is very useful because it helps to know the density properties of a soil when it is not possible to measure ρs with a pycnometer. On the other hand, by knowing the ρs, it is possible to have a quite precise knowledge about the chemistry of the studied soils.178.
Guttikunda Sarath K. Thongboonchoo Narisara Arndt Richard L. Calori Giuseppe Carmichael Gregory R. Streets David G. 《Water, air, and soil pollution》2001,131(1-4):383-406
Sulfur transport and deposition in Asia, on an annual andseasonal basis, is analyzed using the ATMOS model. Calculationsare performed for two complete years (1990 and 1995). Deposition amounts in excess of 0.5 g S m-2 yr-1 are estimated for large regions in Asia, with values as high as 10 g S m-2 yr-1 in southeastern China. Annual averaged SO2 concentrations in excess of 20 μg SO2 m-3 are calculated for many urban and suburban areas ofeastern China and S. Korea, with an average of 5 μg SO2 m-3 over most of the emitter regions. Sulfur deposition by major source categories is also studied. Southeast Asia (Indonesia, Malaysia, Philippines, Singapore)receives ~25% of its sulfur deposition from shipping activities. Sulfur deposition from bio-fuel burning is significant for most of the underdeveloped regions in Asia. Volcanoes are a major source of sulfur emissions in the PacificOcean, Papua New Guinea, Philippines and Southern Japan. Sulfur deposition is shown to vary significantly throughout the year.The monsoons are found to be the largest factor controlling sulfur transport and deposition in the Indian sub-continent andSoutheast Asia. India receives over 35% of its total depositionduring the summer months. In East Asia, sulfur deposition isestimated to be 10% higher during summer and fall than winterand spring. Model results are compared with observations from a number of monitoring networks in Asia and are found to be generally consistent with the limited observations. 相似文献
179.
Carmichael Gregory R. Hayami Hiroshi Calori Giuseppe Uno Itsushi Cho Seog Yeon Engardt Magnuz Kim Seung-Bum Ichikawa Yoichi Ikeda Yukoh Ueda Hiromasa Amann Markus 《Water, air, and soil pollution》2001,130(1-4):51-62
To help improve the use of models in science & policy analysis in Asia it is necessary to have a better understanding of model performance and uncertainties. Towards this goal an intercomparison exercise has been initiated as a collaborative study of scientists interested in long-range transport in East Asia. An overview of this study is presented in this paper. The study consists of a set of prescribed test calculations with carefully controlled experiments. Models used the same domain, emission inventory, model parameters, meteorological conditions, etc. Two periods (January and May 1993) were selected to reflect long-range transport conditions under two distinct seasons. During these periods measurements of sulfur concentrations and deposition were made throughout the study region using identical sampling and analysis protocols. The intercomparison activity consists of four tasks (Blind Test, Fixed Parameter Test, Source Receptor test, and Tuning Test). All participants were asked to do Task A, and as many of the other tasks as possible. To date seven different models have participated in this study. Results and key findings are presented. 相似文献
180.