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ObjectiveTo investigate the contribution of K+ channels on peripheral antinociception induced by ketamine.Study designProspective experimental study.Animals110 male Wistar rats weighing 160–200 g.MethodsThe paw pressure required to elicit limb flexion was designated as the nociceptive threshold. Hyperalgesia was induced by intraplantar injection of prostaglandin E2. All drugs were administered locally into the right hind paw of rats. Ketamine was administered into the right hind paw 2 hours and 55 minutes after local injection of PGE2. Tetraethylammonium was administered 30 minutes prior to ketamine and the other K+ channel blockers, glibenclamide, dequalinium and paxilline, were administered 5 minutes prior to ketamine.ResultsProstaglandin E2 (2 μg per paw) induced hyperalgesia. Ketamine (10, 20, 40 and 80 μg per paw) elicited a local peripheral antinociceptive effect that was antagonized by a specific blocker of ATP‐sensitive K+ channels, glibenclamide (20, 40 and 80 μg per paw). In another experiment, the non‐selective voltage‐dependent K+ channel blocker tetraethylammonium (30 μg per paw) and small and large conductance blockers of Ca2+‐activated K+ channels, dequalinium (50 μg per paw) and paxilline (20 μg per paw), were ineffective at blocking the effect of a local ketamine injection.Conclusions and clinical relevanceAnalysis of these results provides evidence that ketamine, may in part, induce peripheral antinociceptive effects by ATP‐sensitive K+ channel pathway activation. 相似文献
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M. F. Amaral J. C. Mezzalira C. Bremm J. K. Da Trindade M. J. Gibb R. W. M. Suñe P. C. de F. Carvalho 《Grass and Forage Science》2013,68(2):271-277
This study tested the hypothesis that different sward structures, which were constructed by varying the pre‐ and post‐grazing sward heights of annual ryegrass pasture (Lolium multiflorum Lam.) in southern Brazil, affect the short‐term intake rate (STIR) by dairy cows. Treatments consisted of four sward‐management strategies defined by a combination of two pre‐ (25 and 15 cm) and two post‐grazing sward heights (10 and 5 cm): 15‐05, 15‐10, 25‐05 and 25‐10. A completely randomized block design with four replicates was used for the experimental design. The STIR was determined by the double‐weighing technique. Jaw movements were evaluated using automatic recorders (IGER Behaviour Recorder). The results showed that treatment 25‐10 allowed the animals to collect more herbage with a greater bite mass and thus resulted in a greater STIR. Treatments in which sward height was reduced to 10 cm generally favoured the ingestion process than treatments with a post‐grazing sward height of 5 cm. 相似文献
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Karen Gibb Jennifer Beard Peter OReagain Keith Christian Valeria Torok Kathy Ophel-Keller 《Pedobiologia》2008,51(5-6):445-461
An urgent need exists for indicators of soil health and patch functionality in extensive rangelands that can be measured efficiently and at low cost. Soil mites are candidate indicators, but their identification and handling is so specialised and time-consuming that their inclusion in routine monitoring is unlikely. The aim of this study was to measure the relationship between patch type and mite assemblages using a conventional approach. An additional aim was to determine if a molecular approach traditionally used for soil microbes could be adapted for soil mites to overcome some of the bottlenecks associated with soil fauna diversity assessment. Soil mite species abundance and diversity were measured using conventional ecological methods in soil from patches with perennial grass and litter cover (PGL), and compared to soil from bare patches with annual grasses and/or litter cover (BAL). Soil mite assemblages were also assessed using a molecular method called terminal-restriction fragment length polymorphism (T-RFLP) analysis. The conventional data showed a relationship between patch type and mite assemblage. The Prostigmata and Oribatida were well represented in the PGL sites, particularly the Aphelacaridae (Oribatida). For T-RFLP analysis, the mite community was represented by a series of DNA fragment lengths that reflected mite sequence diversity. The T-RFLP data showed a distinct difference in the mite assemblage between the patch types. Where possible, T-RFLP peaks were matched to mite families using a reference 18S rDNA database, and the Aphelacaridae prevalent in the conventional samples at PGL sites were identified, as were prostigmatids and oribatids. We identified limits to the T-RFLP approach and this included an inability to distinguish some species whose DNA sequences were similar. Despite these limitations, the data still showed a clear difference between sites, and the molecular taxonomic inferences also compared well with the conventional ecological data. The results from this study indicated that the T-RFLP approach was effective in measuring mite assemblages in this system. The power of this technique lies in the fact that species diversity and abundance data can be obtained quickly because of the time taken to process hundreds of samples, from soil DNA extraction to data output on the gene analyser, can be as little as 4 days. 相似文献
15.
Inappetence is commonly associated with parasitism and has been observed in both housed and pastured ruminants. In seeking a functional explanation for these observations, it has been hypothesised that parasitized animals may feed more selectively in order to proportionally increase the protein content of their diet and thus partially compensate for their reduced feed intake. Support for this theory is found principally in studies in housed animals under carefully controlled experimental conditions. Grazing animals face a far more heterogeneous environment and a multiplicity of potentially confounding factors that could influence diet selection. Controlled grazing of adjacent monocultures of grass and clover can mitigate some of these variables and was used in the current study to examine the dietary preference of dairy heifers with sub-clinical parasitic gastroenteritis when compared to those receiving regular anthelmintic treatments. Grazing behaviour and herbage intake rates were determined through the use of jaw-movement recorders, direct observation and short-term liveweight change. Consistent with previous observations and despite evidence that nematode burdens were low in the untreated control heifers, a reduction in daily grazing time of 56 min (P = 0.054) was observed in the control animals. There was, however, no evidence that the control heifers showed greater preference for clover compared with ryegrass: partial preference for clover was 73.0% in the untreated controls and 75.5% in the treated heifers. Furthermore control heifers were observed grazing the clover swards significantly (P = 0.032) less frequently than the treated heifers. This study provides additional evidence in grazing cattle for parasite-induced inappetence, manifest as a reduction in grazing time and in subtle changes in ingestive behaviour. The observed partial preference for clover in both treated and control cattle was not significantly affected by the level of parasitism. 相似文献
16.
NH Bonadeo J Erland D Gammon D Park DS Katzer DG Steel 《Science (New York, N.Y.)》1998,282(5393):1473-1476
Picosecond optical excitation was used to coherently control the excitation in a single quantum dot on a time scale that is short compared with the time scale for loss of quantum coherence. The excitonic wave function was manipulated by controlling the optical phase of the two-pulse sequence through timing and polarization. Wave function engineering techniques, developed in atomic and molecular systems, were used to monitor and control a nonstationary quantum mechanical state composed of a superposition of eigenstates. The results extend the concept of coherent control in semiconductors to the limit of a single quantum system in a zero-dimensional quantum dot. 相似文献
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Extract Madam: — We recently treated a goat poisoned by the lily of the valley tree. On Thursday the 30th of December, an adult Angora buck was presented with the following symptoms: projectile vomiting, ataxia, depression, excessive salivation, toxic mucous membranes, weak ruminal movements and a subnormal temperature. The vomiting was spectacular and included numerous leaves of one species of plant. 相似文献
20.
We tested the effects of forest management type (managed vs. unmanaged) on the abundances of Ips typographus (L.) (Col., Curculionidae, Scolytinae) and related parasitoids and predators reared from spruce bolts exposed in Białowieża,
Poland. Habitat availability for these species differed between forest types mainly as a result of regular sanitary fellings
occurring in managed forests. Populations of the common polyphagous insect predators Paromalus paralellepipedus (Herbst) and Plegaderus vulneratus (Panz.) (Col., Histeridae) or parasitoids Rhopalicus tutela (Walk.), Dinotiscus eupterus (Walk.) and Roptrocerus xylophagorum Ratz. (Hym., Pteromalidae) related to I. typographus did not differ between managed and unmanaged forests. Only Thanasimus spp. (Col., Cleridae) was significantly more abundant in managed forest, possibly because it was favored by the more open,
sunny stands. However, the experiment was performed in stands where spruce is only a minor component and during the non-epidemic
phase of the bark beetle population, so further experiments may be necessary to determine if the effects of sanitary fellings
are greater in different forest types and at different stages of the population cycle. 相似文献