全文获取类型
收费全文 | 1987篇 |
免费 | 101篇 |
国内免费 | 1篇 |
专业分类
林业 | 101篇 |
农学 | 68篇 |
基础科学 | 9篇 |
380篇 | |
综合类 | 248篇 |
农作物 | 144篇 |
水产渔业 | 154篇 |
畜牧兽医 | 740篇 |
园艺 | 53篇 |
植物保护 | 192篇 |
出版年
2023年 | 10篇 |
2021年 | 27篇 |
2020年 | 41篇 |
2019年 | 37篇 |
2018年 | 31篇 |
2017年 | 35篇 |
2016年 | 38篇 |
2015年 | 33篇 |
2014年 | 52篇 |
2013年 | 94篇 |
2012年 | 92篇 |
2011年 | 134篇 |
2010年 | 55篇 |
2009年 | 71篇 |
2008年 | 100篇 |
2007年 | 117篇 |
2006年 | 121篇 |
2005年 | 93篇 |
2004年 | 101篇 |
2003年 | 80篇 |
2002年 | 84篇 |
2001年 | 32篇 |
2000年 | 31篇 |
1999年 | 29篇 |
1998年 | 18篇 |
1997年 | 21篇 |
1996年 | 18篇 |
1995年 | 13篇 |
1994年 | 18篇 |
1993年 | 16篇 |
1992年 | 32篇 |
1991年 | 12篇 |
1990年 | 28篇 |
1989年 | 25篇 |
1988年 | 16篇 |
1987年 | 21篇 |
1986年 | 18篇 |
1985年 | 25篇 |
1984年 | 25篇 |
1982年 | 10篇 |
1981年 | 17篇 |
1980年 | 11篇 |
1979年 | 11篇 |
1978年 | 12篇 |
1976年 | 16篇 |
1975年 | 10篇 |
1974年 | 14篇 |
1973年 | 12篇 |
1969年 | 8篇 |
1967年 | 11篇 |
排序方式: 共有2089条查询结果,搜索用时 15 毫秒
991.
Schotte F Lim M Jackson TA Smirnov AV Soman J Olson JS Phillips GN Wulff M Anfinrud PA 《Science (New York, N.Y.)》2003,300(5627):1944-1947
We report picosecond time-resolved x-ray diffraction from the myoglobin (Mb) mutant in which Leu29 is replaced by Phe (L29Fmutant). The frame-by-frame structural evolution, resolved to 1.8 angstroms, allows one to literally "watch" the protein as it executes its function. Time-resolved mid-infrared spectroscopy of flash-photolyzed L29F MbCO revealed a short-lived CO intermediate whose 140-ps lifetime is shorter than that found in wild-type protein by a factor of 1000. The electron density maps of the protein unveil transient conformational changes far more dramatic than the structural differences between the carboxy and deoxy states and depict the correlated side-chain motion responsible for rapidly sweeping CO away from its primary docking site. 相似文献
992.
Daley GQ Ahrlund Richter L Auerbach JM Benvenisty N Charo RA Chen G Deng HK Goldstein LS Hudson KL Hyun I Junn SC Love J Lee EH McLaren A Mummery CL Nakatsuji N Racowsky C Rooke H Rossant J Schöler HR Solbakk JH Taylor P Trounson AO Weissman IL Wilmut I Yu J Zoloth L 《Science (New York, N.Y.)》2007,315(5812):603-604
993.
Demyelination results in severe disability in many neurodegenerative diseases and nervous system infections, and it is typically mediated by inflammatory responses. Mycobacterium leprae, the causative organism of leprosy, induced rapid demyelination by a contact-dependent mechanism in the absence of immune cells in an in vitro nerve tissue culture model and in Rag1-knockout (Rag1-/-) mice, which lack mature B and T lymphocytes. Myelinated Schwann cells were resistant to M. leprae invasion but undergo demyelination upon bacterial attachment, whereas nonmyelinated Schwann cells harbor intracellular M. leprae in large numbers. During M. leprae-induced demyelination, Schwann cells proliferate significantly both in vitro and in vivo and generate a more nonmyelinated phenotype, thereby securing the intracellular niche for M. leprae. 相似文献
994.
Baumeister W Bachmair A Chau V Cohen R Coffino P Demartino G Deshaies R Dohmen J Emr S Finley D Hampton R Hill C Hochstrasser M Huber R Jackson P Jentsch S Johnson E Kwon YT Pagano M Pickart C Rechsteiner M Scheffner M Sommer T Tansey W Tyers M Vierstra R Weissman A Wilkinson KD Wolf D 《Science (New York, N.Y.)》2004,306(5700):1290-1292
995.
George S Rochford JJ Wolfrum C Gray SL Schinner S Wilson JC Soos MA Murgatroyd PR Williams RM Acerini CL Dunger DB Barford D Umpleby AM Wareham NJ Davies HA Schafer AJ Stoffel M O'Rahilly S Barroso I 《Science (New York, N.Y.)》2004,304(5675):1325-1328
Inherited defects in signaling pathways downstream of the insulin receptor have long been suggested to contribute to human type 2 diabetes mellitus. Here we describe a mutation in the gene encoding the protein kinase AKT2/PKBbeta in a family that shows autosomal dominant inheritance of severe insulin resistance and diabetes mellitus. Expression of the mutant kinase in cultured cells disrupted insulin signaling to metabolic end points and inhibited the function of coexpressed, wild-type AKT. These findings demonstrate the central importance of AKT signaling to insulin sensitivity in humans. 相似文献
996.
997.
998.
999.