大豆脂肪含量与生态因子关系的研究

2001～2002年在河南省夏大豆主产区的5个试点,以豫豆25号13期分期播种的方法,研究了气象、土壤养分和海拔等38个生态因子对豫豆25号脂肪含量的关系,通过逐步回归统计分析,筛选出与大豆脂肪含量密切相关的11个生态因素间的直线或曲线关系.明确了夏大豆鼓粒成熟期较少的日照、较高的均温、较多的降水和较大的昼夜温差以及出苗期较高的均温和花荚期较多的降水利于脂肪的积累形成.较高的土壤全氮和钾含量、较低的硫含量有利于大豆脂肪大幅度提高.在本试验pH值6.95～7.89范围内,偏碱性土壤利于脂肪的形成.在本试验研究因子范围内,其它生态因子对大豆脂肪含量无明显影响.     

高产优质抗病虫棉花品种冀2658

冀2658是2015年通过河北省审定的高产、优质、抗病虫棉花品种。本文概述了其选育过程、特征特性及栽培的技术要点。     

NMR-Based Metabolomic Analysis of Spatial Variation in Soft Corals

Soft corals are common marine organisms that inhabit tropical and subtropical oceans. They are shown to be rich source of secondary metabolites with biological activities. In this work, soft corals from two geographical locations were investigated using ¹H-NMR spectroscopy coupled with multivariate statistical analysis at the metabolic level. A partial least-squares discriminant analysis showed clear separation among extracts of soft corals grown in Sanya Bay and Weizhou Island. The specific markers that contributed to discrimination between soft corals in two origins belonged to terpenes, sterols and N-containing compounds. The satisfied precision of classification obtained indicates this approach using combined ¹H-NMR and chemometrics is effective to discriminate soft corals collected in different geographical locations. The results revealed that metabolites of soft corals evidently depended on living environmental condition, which would provide valuable information for further relevant coastal marine environment evaluation.     

Determination of melengestrol acetate in bovine tissue: collaborative study.

Seven laboratories collaboratively studied a method for the assay of melengestrol acetate at the 0, 10, and 20 ppb levels in bovine fat, liver, muscle, and kidney. The study included fortification of tissue by each laboratory and analysis of fat samples taken from treated heifers which had endogenous levels of 0, 10, and 20 ppb melengestrol acetate. The multistep cleanup procedure used included extraction, solvent partition, column chromatography, and electron capture gas-liquid chromatographic, determination. Results of the study for muscle, liver, kidney, and fat showed that the method gave satisfactory recoveries and accuracy. In fat, the most critical tissue, recovery was greater than 93%. A statistical comparison of the results reported for fat tissue from treated heifers demonstrated that 5 of the 7 laboratories obtained similar results. The results produced by the method can be expected to be repeatable within and among laboratories. On the basis of the collaborative results the method has been adopted as official first action.     

作物品质性状的分子遗传改良

近年来,人们对蔬菜、水果和农作物的品质要求不断提高,而品质性状多为多基因或寡基因控制的数量性状或数量-质量性状,其表型易受到环境影响。一些品质基因常表现隐性且品质检测操作复杂,制约了品质育种的发展。随着分子标记技术的发展,寻找与优良品质性状紧密连锁的分子标记,并用以进行标记辅助选择育种,将大大提高品质性状选择效率和遗传改良速度,分子标记、分子标记辅助育种和转基因技术将为改善作物品质提供有效手段。总体来说,作物品质在分子水平上的研究尚处于起步阶段,但取得了一些令人鼓舞的成果,本文对小麦、水稻、黄瓜、番茄、西瓜等作物外观品质、营养品质和风味品质在分子水平上的研究进展进行了综述,并探讨转基因技术和分子标记辅助育种在改善作物品质性状方面的前景。     

地高辛标记核酸探针检测重组人白细胞介素1受体颉颃剂中DNA残余量的研究

试验旨在建立重组人白细胞介素1受体颉颃剂(interleukin-1 receptor antagonist,IL-1ra)原液中宿主菌DNA残余量的检测方法。以重组白细胞介素1受体颉颃剂工程菌基因组DNA为模板,优化试验条件,借助超声波手段,制备地高辛标记的核酸探针, 并以此探针进行狭缝杂交及显色反应。结果显示,在24 批次重组人IL-1ra原液中,每人份使用剂量的宿主DNA残余量均小于10 ng。结果表明,该方法灵敏度较高、特异性较强、重复性较好、操作步骤安全且较为方便,可用于重组人IL-1ra原液及半成品的DNA残余检测。     

景天科观赏植物“巴”的叶片扦插技术

作为一种新型的观赏植物,"巴"近几年被人们从遥远的东非引进中国,它不但具有很强的观赏特征,同时人们发现它特殊的代谢方式更是对居室环境和人们的身体健康极为有益。但是,由于是原产地气候环境里的特殊植物—在新的环境里很难收获种子,有性繁殖困难,同时它具有明显的休眠特征,且体内富含水分,给无性繁殖也带来了很大的难度,在经过一系列的试验后,终于找到了用"巴"的叶片进行无性繁殖的成功途径。     

Soil bacterial community structure and extracellular enzyme activities under different land use types in a long-term reclaimed wetland

Journal of Soils and Sediments - This study was conducted to investigate shifts in the bacterial community structure and soil enzyme activities during long-term reclamation of different land use...     

氰烯菌酯与己唑醇对小麦纹枯病菌联合毒力测定

为明确氰烯菌酯与己唑醇不同比例混配对小麦纹枯病菌联合作用类型,采用菌丝生长速率法测定了氰烯菌酯与己唑醇及其5种配比对小麦纹枯病菌的毒力。结果表明,氰烯菌酯与己唑醇及其1:5、1:7、1:9、1:11、1:13等混配组合对禾谷丝核菌的EC50分别是0.212、0.339、0.227、0.207、0.213、0.315、0.320 mg/L,5种混配组合对禾谷丝核菌的增效系数(SR)分别是1.35、1.52、1.51、1.02、1.02;对立枯丝核菌的EC50分别是0.452、2.984、1.091、1.139、1.217、1.280、1.781 mg/L,增效系数(SR)分别是1.41、1.54、1.57、1.59、1.20;氰烯菌酯与己唑醇1:(7~9)混配对小麦纹枯病菌有增效作用。