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21.
Y Henry B Sève Y Colléaux P Ganier C Saligaut P Jégo 《Journal of animal science》1992,70(6):1873-1887
The effects of dietary level of tryptophan (TRP) and CP content and composition on voluntary feed intake, growth performance, and carcass characteristics in finishing pigs were studied in two experiments, with an equal number of females and castrated males. In Exp. 1, involving 120 Large White pigs from 44 to 99 kg BW with ad libitum access to feed, six treatments were compared according to a 2 x 3 factorial arrangement: 1) two levels of TRP (.09 and .13%), suboptimal and optimal for growth, respectively, 2) three types of CP supply (a 12.5% CP diet based on corn-soybean meal, and adequately balanced for essential amino acids [EAA] other than TRP; 15.7% CP diet with additional protein from corn gluten meal; 16.2% CP diet with additional nonessential amino acids [NEAA, in the form of L-glutamic acid.HCl and glycine], and the same levels of EAA as in the 12.5% CP diet. In Exp. 2, including four of the six previous factorial combinations (.09 and .13% TRP, 12.3 and 15.8% CP with additional protein), 32 pigs of 50-kg initial BW were used during 21 d, and further observations on meat quality characteristics, plasma free amino acid levels, and serotonin concentrations in the posterior hypothalamus were made. The major observed effects were interactions of different magnitude according to sex between TRP level and the amount and the composition of additional CP. At the suboptimal level of .09% TRP, the increase in protein content severely decreased daily feed intake and growth compared with the .13% level, especially in females. Conversely, the addition of NEAA at both TRP levels had little effect on daily feed intake and growth. Deficiency of TRP exerted a significant increase of pH in adductor femoris and semimembranosus muscles measured 45 min and 24 h postmortem, but only in females. Voluntary feed intake, as affected by dietary TRP and CP levels, was linearly related with concomitant changes in TRP to large neutral amino acids (TRP:LNAA) ratio both in feed and in plasma, which in turn was directly associated to hypothalamic serotonin concentration. It was concluded that an overly low concentration of serotonin in the hypothalamus, especially in females, as a result of TRP:LNAA imbalance, could be involved in the reduction of voluntary feed intake. 相似文献
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A technique for creating critical-size defects in the metatarsus of sheep for use in investigation of healing of long-bone defects 总被引:2,自引:0,他引:2
Viateau V Guillemin G Yang YC Bensaid W Reviron T Oudina K Meunier A Sedel L Petite H 《American journal of veterinary research》2004,65(12):1653-1657
OBJECTIVE: To develop a technique for use in investigation of healing of long-bone defects by creation of a critical-size defect in the left metarsal III and IV bone (metatarsus) of sheep. ANIMALS: 18 healthy adult sheep. PROCEDURE: Sheep were allocated to 4 groups (3, 3, 5, and 7 sheep in groups 1 to 4, respectively). An ostectomy with various segmental length-to-diaphyseal diameter ratios (0.5, 1.0, 2.0, and 2.0 for groups 1 to 4, respectively) was performed on the left metatarsus of each sheep. The defect was left empty in sheep of groups 1, 2, and 3, whereas the defect was filled with a massive corticocancellous bone autograft in sheep of group 4. RESULTS: All sheep tolerated the surgical procedure well and were able to use the affected limb the day after surgery. Radiographic and histologic examinations conducted 16 weeks after surgery revealed nonunion in all sheep of groups 1, 2, and 3, whereas consistent bone healing with abundant bone formation was observed in all sheep of group 4. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of these findings suggests that the sheep metatarsal model is a critical-size defect model with low morbidity. It should allow the assessment of new technologies for bone regeneration in conditions closely mimicking the clinical setting. IMPACT FOR HUMAN MEDICINE: Use of this technique in sheep should be of benefit for the preclinical study of osteoconductive, osteoinductive, or osteogenic biomaterials for use in humans. 相似文献
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Eve Ramery Stratos Papakonstantinou Manuel Pinilla Hester McAllister Hanne Jahns Barbara Gallagher Peter J. O��Brien 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(4):423-425
A 9-year-old female Yorkshire terrier was presented for vomiting and diarrhea. Blood chemistry tests revealed hepatic dysfunction, cholestasis, and inflammation. Liver ultrasonography and liver biopsy were consistent with cholangiohepatitis. Fine-needle aspiration of the gallbladder revealed the presence of bacteria later identified as Clostridium spp. The cholangiohepatitis was successfully treated. 相似文献
26.
Olga G��jska-Zygner Roman Lechowski Wojciech Zygner 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(6):623-625
An 11-year-old, 24-kg, intact female Siberian husky dog in anestrus had a 2-month history of polyuria and polydipsia. The dog had signs of mineralocorticoid excess such as hypertension and hypokalemia refractory to potassium supplementation. Abdominal ultrasound revealed an irregular mass in the left adrenal gland. The ACTH stimulation test for aldosterone concentration did not reveal hyperaldosteronism. Unilateral adrenalectomy was performed and histopathology identified adrenal cortical carcinoma. All clinical signs of mineralocorticoid excess ceased after surgery. 相似文献
27.
Favrot C Steffan J Seewald W Hobi S Linek M Marignac G Olivry T Beco L Nett C Fontaine J Roosje P Bergvall K Belova S Koebrich S Pin D Kovalik M Meury S Wilhelm S 《Veterinary dermatology》2012,23(1):45-50, e11
Hypersensitivity dermatitides (HD) are commonly seen in cats, and they are usually caused by environmental, food and/or flea allergens. Affected cats normally present with one of the following clinical reaction patterns: head and neck excoriations, usually symmetrical self-induced alopecia, eosinophilic skin lesions or miliary dermatitis. Importantly, none of these clinical presentations is considered to be pathognomonic for HD skin diseases, and the diagnosis of HD is usually based on the exclusion of other pruritic diseases and on a positive response to therapy. The objectives of this study were to propose sets of criteria for the diagnosis of nonflea-induced HD (NFHD). We recruited 501 cats with pruritus and skin lesions and compared clinical parameters between cats with NFHD (encompassing those with nonflea, nonfood HD and those with food HD), flea HD and other pruritic conditions. Using simulated annealing techniques, we established two sets of proposed criteria for the following two different clinical situations: (i) the diagnosis of NFHD in a population of pruritic cats; and (ii) the diagnosis of NFHD after exclusion of cats with flea HD. These criteria sets were associated with good sensitivity and specificity and may be useful for homogeneity of enrolment in clinical trials and to evaluate the probability of diagnosis of NFHD in clinical practice. Finally, these criteria were not useful to differentiate cats with NFHD from those with food HD. 相似文献
28.
Ana M. Guti��rrez Jos�� J. Cer��n Blas A. Marsilla Mar��a D. Parra Silvia Martinez-Subiela 《Canadian journal of veterinary research》2012,76(2):136-142
A new method was developed to simultaneously measure 2 acute-phase proteins (APPs) by time-resolved immunofluorometry. The assay, based on double-label quantification of haptoglobin (Hp) and C-reactive protein (CRP) in meat juice samples from pigs, was constructed by use of a combination of europium and samarium chelate lanthanides as labels. Meat juice samples from 154 pigs were used for analytic and clinical validation of the assay through determination of precision, accuracy, limit of detection, and quantification. The analytic performance of the assay was satisfactory, with good intra-assay and interassay precision and accuracy. The levels of Hp and CRP were increased in the meat juice samples of diseased animals compared with healthy ones. According to the results, higher sensitivity could be achieved if the cut-off values of both proteins were taken into account for clinical relevance rather than used individually. Since the dual assay saved both time and sample, it could be used as a rapid and sensitive screening test in porcine production. 相似文献
29.
Marco Quartuccio Gabriele Marino Giuseppe Garufi Santo Cristarella Antonina Zangh�� 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(2):207-209
The association of cryptorchidism, functional Sertoli cell tumors, and spermatic cord torsion has been rarely reported in the literature. Two dogs were admitted for bilateral skin alopecia and weight loss. Both animals were cryptorchid and displayed a pendulous preputial sheath, prostate hypertrophy, and increased levels of circulating oestrogen. Transabdominal palpation and ultrasonography revealed the presence of neoplastic retained gonads. During surgery, spermatic cord torsion was also detected in the enlarged neoplastic testes of both dogs. Histologic examination confirmed the presence of Sertoli cell tumors that were primarily responsible for the feminizing syndrome. Complete remission of all symptoms occurred within 3 months after orchiectomy. 相似文献
30.