Nonspecific immunostimulation against viruses

A trypsinized preparation from chromogenic selected strain of Mycobacterium phlei (NSI) stimulated the recipient immune system non-specifically against a variety of viruses viz. Rabies virus (RNA virus). Marek's disease (DNA virus) and Foot and Mouth disease virus (RNA virus) in phylogenetically different hosts like mice, chicks and guinea pigs respectively. Investigation into mechanisms of such nonspecific immunostimulation revealed that there was induction of strong cell mediated immune response (CMIR) against both specific as well as to nonspecific viral antigens as evinced by LMIT, lymphokine (LyIF) assay and lymphocyte transformation test etc. There was induction of appreciable quantity of "Infectivity inhibiting substance(s)" (IIS) which was not the classical antibody (Ig) in the serum of NSI inoculated animals and birds. This substance(s) neutralized FMD, IBR, rabies and Newcastle disease virus in cell culture, mice and in embryonated eggs. Electrophoretic separation of the NSI induced serum revealed an increase in beta fraction of globulin.     

An epidemiological investigation of an idiopathic myopathy in hunting dogs in New Zealand

AIM: To conduct an epidemiological investigation of an idiopathic myopathy, known as “Go Slow” (GSM), which was initially recognised in dogs used for pig hunting. A secondary aim was to describe the hunting activities, diet and health of dogs used for pig hunting in New Zealand.

METHODS: A retrospective cohort study was conducted between June 2014–June 2017. Cases of GSM in dogs were diagnosed by veterinarians using a combination of clinical history, physical examination findings, serum biochemistry and/or skeletal muscle histology. A telephone interview was conducted with the owner or primary veterinarian to provide information regarding the dog’s diet and exercise over the 7 days preceding the onset of clinical signs. In August 2015, a separate online survey of owners of dogs used for pig hunting was conducted to characterise the normal hunting activities, diet and health of these dogs.

RESULTS: A total of 86 cases of GSM were recruited, of which 58 (67%) were pig hunting dogs, 16 (19%) pet dogs and 12 (14%) working farm dogs. Cases were most commonly reported in the upper North Island, and 65 (76 (95% CI=67–85)%) were from the Northland region. Processed commercial dog food had been fed to 93 (95% CI=88–98)% of affected dogs. Ingestion of raw, frozen or cooked wild pig in the preceding week was reported for 76 (88 (95% CI=82–95)%) dogs with the myopathy. In the survey of owners of healthy pig hunting dogs, 203 eligible responses were received; pig hunting was reported to most commonly occur in Northland (20.2%), Waikato (22.3%) and Bay of Plenty (23.2%) regions. Commercial dog food was fed to 172 (85 (95% CI=80–90)%) of the dogs included in this survey, and 55 (27 (95% CI=20–33)%) had eaten wild pig in the preceding week. The most common reported health problem in pig hunting dogs was traumatic wounds.

CONCLUSIONS: Cases of GSM were most commonly recognised in dogs used for pig hunting, but also occurred in pet and working farm dogs. The disease was most frequently reported in the upper North Island of New Zealand and ingestion of wild pig was a consistent feature in cases of this myopathy.

CLINICAL RELEVANCE: To minimise the risk of dogs developing this myopathy, it would seem prudent to avoid feeding any tissues from wild pigs to dogs in areas where the disease is known to occur.     

Egg production performance,egg yolk antioxidant profile and excreta concentration of corticosterone in golden pheasants (Chrysolophus pictus) fed diets containing different levels of green vegetables

In order to study the effect of feeding graded levels of green vegetables on egg production performance and egg yolk antioxidant status, 27 female golden pheasants (GP) were randomly distributed into three groups of nine each in an experiment based on completely randomized design. The diets of the birds in groups T₁, T₂ and T₃ contained 1.4%, 2.7% and 5.0% of green vegetables respectively. Feeding of experimental diets started on 12th February (day 1) and was continued till 30 June 2012. Average number of eggs laid and egg mass produced by the hens in group T₃ was higher (p < 0.004) than those of T₁ and T₂. Hen day egg production was lowest (p < 0.001) in group T₁. Roche yolk colour score was highest (p < 0.01) in T₃ followed by T₂ and was lowest in T₁. Other external and internal egg quality parameters were similar among the groups. Total antioxidant capacity (TAC) of egg yolk was higher (p < 0.04) in group T₃ as compared to other two groups. Egg yolk concentration of total carotenoids, β‐carotene, total sulfhydryl, protein‐bound sulfhydryl and non‐protein‐bound sulfhydryl was higher (p < 0.05), whereas concentration of malondialdehyde was lower (p < 0.024) in group T₃ as compared to other two groups. Excreta concentration of corticosterone was highest (p < 0.012) in T₁, followed by T₂, and was lowest in T₃. Egg yolk concentration of TAC and TSH was positively and that of malondialdehyde was negatively correlated with carotenoids intake. Excreta concentration of corticosterone was negatively correlated with carotenoids intake. It was concluded that incorporation of green vegetables in the diet at 5% on dry matter basis would improve egg production, egg yolk antioxidant status and the ability of the captive GP to combat stress.     

Assessing forest fragmentation in north-western Himalaya: a case study from Ranikhet forest range,Uttarakhand, India

The northwestern Himalaya harbors high levels of biodiversity due to its unique topography, climatic conditions and heterogeneity. Forest fragmentation is one of the major threats causing a decline in biodiversity in the Himalayan region. We assesses forest fragmentation and changes in land use land cover(LULC) patterns using multi-temporal satellite data over a time span of four decades(1976–2013). Fragmentation analysis using the Landscape Fragmentation Tool(LFT) reveals a decrease in core and edge areas by 14 and 2.3 %, respectively; while an increase in non-forest, patch area and perforation area by 2.1, 0.4, and 14 %, respectively. The LULC dynamics show that the areas under dense forest and scrub forest have decreased by 2.8 % and 1.9 %, respectively; and there is an increase in open forest, crop land and fallow land area by 2.6, 1.7 and 2.1 %, respectively. The quantification of landscape heterogeneity is undertaken with the help of landscape metrics computed using FRAGSTATS at class and landscape level, showing signs of increased fragmentation. Our study provides baseline database that can support the future biodiversity conservation and sustainable forest management initiatives.     

Effects of N‐acetyl‐cysteine and N‐acetyl‐cysteine‐amide Supplementation on In Vitro Matured Porcine Oocytes

This study was conducted to evaluate the effects of different concentrations of the antioxidant N‐acetyl‐cysteine (NAC) supplemented to the maturation medium on porcine embryo development. Concentrations of NAC and its synthetic derivative, NAC‐amide (NACA) were evaluated for effects on nuclear maturation, fertilization success and embryo development. Concentrations of NAC (0, 0.5, 1.0, 1.5, 2.0, 2.5 and 5.0 mm ) were supplemented to maturing oocytes, and embryo development was analysed at 48 and 144 h post‐fertilization. There were no differences among cleavage rates for any of the treatment groups. Blastocyst formation for 1.5 mm NAC (56.5 ± 9.2%) was higher (p < 0.05) than all other supplementations. There were no differences in nuclear maturation or fertilization or in cleavage rates when comparing 1.5 mm NAC and 1.5 mm NACA supplementation to the control. Blastocyst formation for 1.5 mm NAC (44.4 ± 4.7%) and 1.5 mm NACA (46.2 ± 3.4%) supplementation were higher (p < 0.05) than the control (32.1 ± 6.2%) oocytes. These results indicate that supplementing 1.5 mm of NAC or NACA to the oocyte maturation medium increased the percentage of viable embryos reaching the blastocyst stage of development.     

Organizational dynamics of an information system： case study from the forestry sector

The Forest Department in the State of Uttar Pradesh, India developed Forest Management Information System （FMIS） for achieving organizational goals of improved financial and human resource management, improvement in the management of forests and wildlife, and for achieving responsive administration. This paper, based on field research, presents an assessment of the dynamics of FMIS in organizational context for a better understanding of such systems in forestry organizations. The paper also investigates the success of FMIS in assisting decision makers in achieving organizational goals. Based on the knowledge developed during the course of the study, key learning elements have been highlighted for the benefit of the stakeholders in information systems in forest sector.     

Effect of fertilization frequency on pond productivity and fish biomass in still water ponds stocked with Cirrhinus mrigala (Ham.)

Four different fertilization frequencies, namely twice per week, once per week, twice a month and once a month, were used in ponds to assess their effects on nutrient release, pond productivity and fish biomass. All ponds received the same total fertilizer inputs during the experimental period of 60 days (cow dung 208.3 kg ha^?1 week^?1, TSP 9.8 kg ha^?1 week^?1, urea 6.0 kg ha^?1 week^?1). Studies have revealed that the highest values of fish biomass, specific growth rate (SGR), net primary productivity (NPP), plankton population and nutrients were observed in the ponds that were fertilized twice a month. A strong and significant correlation of fertilization frequency was observed with dissolved oxygen, biochemical oxygen demand (BOD), alkalinity, nutrient release, NPP, plankton density (no. L^?1), fish biomass and SGR. The linear relationship between NPP and fish biomass/SGR for all the ponds was strong (r²= 0.88). Sediment chemistry revealed that O‐PO₄, NO₃‐N, organic carbon and electrical conductivity (EC) increased significantly (P<0.05) with a decrease in the frequency of fertilization, while alkalinity and calcium were high in ponds that were fertilized twice a month.