全文获取类型
收费全文 | 1055篇 |
免费 | 94篇 |
国内免费 | 1篇 |
专业分类
林业 | 91篇 |
农学 | 24篇 |
基础科学 | 5篇 |
173篇 | |
综合类 | 74篇 |
农作物 | 67篇 |
水产渔业 | 166篇 |
畜牧兽医 | 452篇 |
园艺 | 13篇 |
植物保护 | 85篇 |
出版年
2024年 | 2篇 |
2023年 | 18篇 |
2022年 | 31篇 |
2021年 | 50篇 |
2020年 | 70篇 |
2019年 | 60篇 |
2018年 | 59篇 |
2017年 | 53篇 |
2016年 | 57篇 |
2015年 | 38篇 |
2014年 | 59篇 |
2013年 | 71篇 |
2012年 | 102篇 |
2011年 | 90篇 |
2010年 | 44篇 |
2009年 | 42篇 |
2008年 | 40篇 |
2007年 | 49篇 |
2006年 | 26篇 |
2005年 | 30篇 |
2004年 | 29篇 |
2003年 | 26篇 |
2002年 | 18篇 |
2001年 | 9篇 |
2000年 | 8篇 |
1999年 | 7篇 |
1998年 | 2篇 |
1997年 | 6篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1993年 | 3篇 |
1992年 | 7篇 |
1991年 | 6篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1965年 | 3篇 |
1963年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有1150条查询结果,搜索用时 31 毫秒
61.
A map of the interactome network of the metazoan C. elegans 总被引:1,自引:0,他引:1
Li S Armstrong CM Bertin N Ge H Milstein S Boxem M Vidalain PO Han JD Chesneau A Hao T Goldberg DS Li N Martinez M Rual JF Lamesch P Xu L Tewari M Wong SL Zhang LV Berriz GF Jacotot L Vaglio P Reboul J Hirozane-Kishikawa T Li Q Gabel HW Elewa A Baumgartner B Rose DJ Yu H Bosak S Sequerra R Fraser A Mango SE Saxton WM Strome S Van Den Heuvel S Piano F Vandenhaute J Sardet C Gerstein M Doucette-Stamm L Gunsalus KC Harper JW Cusick ME Roth FP Hill DE Vidal M 《Science (New York, N.Y.)》2004,303(5657):540-543
62.
Faham S Watanabe A Besserer GM Cascio D Specht A Hirayama BA Wright EM Abramson J 《Science (New York, N.Y.)》2008,321(5890):810-814
Membrane transporters that use energy stored in sodium gradients to drive nutrients into cells constitute a major class of proteins. We report the crystal structure of a member of the solute sodium symporters (SSS), the Vibrio parahaemolyticus sodium/galactose symporter (vSGLT). The approximately 3.0 angstrom structure contains 14 transmembrane (TM) helices in an inward-facing conformation with a core structure of inverted repeats of 5 TM helices (TM2 to TM6 and TM7 to TM11). Galactose is bound in the center of the core, occluded from the outside solutions by hydrophobic residues. Surprisingly, the architecture of the core is similar to that of the leucine transporter (LeuT) from a different gene family. Modeling the outward-facing conformation based on the LeuT structure, in conjunction with biophysical data, provides insight into structural rearrangements for active transport. 相似文献
63.
Understanding the brain computations leading to object recognition requires quantitative characterization of the information represented in inferior temporal (IT) cortex. We used a biologically plausible, classifier-based readout technique to investigate the neural coding of selectivity and invariance at the IT population level. The activity of small neuronal populations (approximately 100 randomly selected cells) over very short time intervals (as small as 12.5 milliseconds) contained unexpectedly accurate and robust information about both object "identity" and "category." This information generalized over a range of object positions and scales, even for novel objects. Coarse information about position and scale could also be read out from the same population. 相似文献
64.
Neuron-glia adhesion is inhibited by antibodies to neural determinants 总被引:10,自引:0,他引:10
Suspensions of embryonic chick neuronal cells adhered to monolayers of glial cells, but few neurons bound to control monolayers of fibroblastic cells from meninges or skin. Neuronal cell-glial cell adhesion was inhibited by prior incubation of the neurons with Fab' fragments of antibodies to neuronal membranes. In contrast, antibodies to the neural cell adhesion molecule (N-CAM) did not inhibit the binding. These results suggest that a specific adhesive mechanism between neurons and glial cells exists and that it is mediated by CAM's that differ from those so far identified. 相似文献
65.
Morin S Savarino J Frey MM Yan N Bekki S Bottenheim JW Martins JM 《Science (New York, N.Y.)》2008,322(5902):730-732
Atmospheric nitrogen oxides (NOx =NO+ NO2) play a pivotal role in the cycling of reactive nitrogen (ultimately deposited as nitrate) and the oxidative capacity of the atmosphere. Combined measurements of nitrogen and oxygen stable isotope ratios of nitrate collected in the Arctic atmosphere were used to infer the origin and fate of NOx and nitrate on a seasonal basis. In spring, photochemically driven emissions of reactive nitrogen from the snowpack into the atmosphere make local oxidation of NOx by bromine oxide the major contributor to the nitrate budget. The comprehensive isotopic composition of nitrate provides strong constraints on the relative importance of the key atmospheric oxidants in the present atmosphere, with the potential for extension into the past using ice cores. 相似文献
66.
Pierrette Fleurat-Lessard Fabienne Dédaldéchamp Florence Thibault Emile Béré Gabriel Roblin 《Scientia Horticulturae》2011
The present study aimed to determine the most efficient experimental conditions of iron sulfate use leading to optimal inhibition in the development of fungal pathogens. Assays have been focused on fungal species inducing severe grapevine diseases. FeSO4 directly inhibited the in vitro mycelial growth of Botrytis cinerea, Eutypa lata, Phaeomoniella chlamydospora, Phaeoacremonium aleophilum, Diplodia seriata, and Neofusicoccum parvum with variable efficiency in the range of 0.5–10 mM. The development was always completely inhibited at 20 mM. This inhibitory effect was greatly increased at acidic pH values. The anionic moiety of the molecule was of importance since bromide, chloride and sulfate were highly active, whereas acetate and oxalate showed a small effect. Electron microscope observations on E. lata and B. cinerea showed that a treatment with FeSO4 induced dramatic changes in the hyphal organization leading to cell death. No toxicity was observed on grapevine leaves following repeated FeSO4 sprays in the antifungal concentration range. Therefore, FeSO4 may be proposed to effectively replace the long-term pollutant use of CuSO4 as an antifungal agent, with the additional advantage of iron being an important plant micronutrient. 相似文献
67.
Sonia Salvo Carlos Muñoz Julio Ávila Jaime Bustos Emilio Cariaga Carolina Silva Gabriel Vivallo 《Scientia Horticulturae》2011
Blueberry exporting is an important activity in Chile, with fresh blueberries commanding the highest prices and being among the most exported products to the European and North American markets. To maintain quality in the centres of consumption, farmers must continuously improve the logistics of harvesting and shipping the blueberries. Thus every year they must calculate the production of the orchard well in advance in order to hire staff and ensure the logistic cold chain. For this calculation they use a count of flower buds and a simple linear model of which the slope parameter represents the number of fruits per bud. However, due to the cost of the counting procedure, some producers count only a fraction of each plant (25%, 50% or 100%), and furthermore they do not know what effect the variety and productive age of the plants may have on the estimation. The objective of this work is to measure the impact of the cultivated variety, the age of the plant in productive years, and the percentage of fruits counted in estimating the parameter fruits per bud. The study involved monitoring 310 plants of different varieties and ages distributed in northern, central and southern Chile (over an area of approximately 700 km × 200 km). The parameter was estimated by fitting simple linear regression models (SLRM) as a function of the number of fruits and flower buds. To evaluate the impact on the parameter, the SLRM was fitted considering the variables observed in all the plants, by percentage counted, by variety and by variety-age of the plant. The major findings indicate significant differences in the estimation of the parameter, suggesting that in order to estimate fruits per bud the whole plant must be counted and its age and variety taken into account. 相似文献
68.
Ryo Kubota Mark A. Schell Gabriel D. Peckham Joanne Rue Anne M. Alvarez Caitilyn Allen Daniel M. Jenkins 《Journal of General Plant Pathology》2011,77(3):182-193
New rapid diagnostic methods are urgently needed to discriminate the quarantine pathogen Ralstonia solanacearum (Rs) race 3 biovar 2 (R3B2) from other populations of Rs that lack the adaptation to cause bacterial wilt disease in temperate
regions. We used an in silico bioinformatic approach to identify several genome sequences potentially specific to R3B2 strains. Primer sets were designed
to PCR-amplify sequences in these regions, and four sets were ultimately shown to be >99% accurate for detection of R3B2 strains.
On the basis of these results, several primers were designed to enable development of a loop-mediated isothermal amplification
assay that was rapid, technologically simple, and essentially 100% accurate for identification of R3B2 when applied to a comprehensive
collection of geographically diverse Rs strains. We fortuitously found that a sequence in one of the “R3B2-specific” regions
has ~90% identity to a sequence present in strains of the blood disease bacterium (BDB), a member of the Rs species complex
that infects banana. Alignments of these sequences allowed design of a second PCR primer set that proved 100% accurate for
identification of BDB strains when tested on the 22 BDB strains available to us. These results demonstrate the power of in silico genomic subtraction for rapid identification of population-specific DNA sequences and for the development of simple, reliable
detection methods for Rs subpopulations. 相似文献
69.
华北农业高产粮区地下水硝酸盐污染环境价值损失评估技术研究——以山东省桓台县为例 总被引:6,自引:0,他引:6
应用人力资本法、防护支出法和支付意愿调查法评估了华北高产粮区桓台县地下水农业面源硝酸盐污染的环境经济损失,并分析了不同评估技术对农业面源硝酸盐污染地下水环境价值损失评估的适用性,其适用性优先序为防护支出法的深井工程法>脱硝工程法≥人力资本法>支付意愿调查法。2 0 0 2年该县由农业地下水硝酸盐污染造成的年环境价值损失约为880万元(86 0 .8~1170 .1万元/浕) ,占当年农业总产值的1.1%~1.5 %。 相似文献
70.
Bernárdez M Pastoriza L Sampedro G Herrera JJ Cabo ML 《Journal of agricultural and food chemistry》2005,53(6):1903-1906
The objective of this study was the development of a method for the quantification of free fatty acids (FFA) using less aggressive reactants against the handler and the environment than those used in the classic method of Lowry and Tinsley. The modified procedure is a variation of the Lowry and Tinsley method employing cyclohexane in place of benzene. The use of benzene is prohibited in certain work processes and laboratories, and the competent authority in each country is actively promoting research into harmless or less harmful products that could replace benzene. A comparison with the traditional AOCS titration method for oil analysis was performed. FFA content in mackerel frozen at -10 degrees C was measured according to the three methods over a 12 month period. The results showed similar values, and good correlations were obtained. 相似文献