针叶树苗圃病害调查与主要病害发生规律研究

本文调查了针叶树苗期病害的种类和苗期病害的发生规律，结果表明针叶树苗期病害有8种，其中侵染性病害有6种，生理性病害有2种。猝倒病是针叶树苗期主要的病害，表现为3种症状类型。日灼型猝倒是气象性病害的主要形式，由丝核菌Rhizoctonia和镰刀菌Fusarium引起的生物猝倒型和立枯型病害是针叶树育苗的主要病害，其发生与育苗地所处的环境、营养土基质有关,同时提出了对针叶树苗期病害防治的对策。     

Stand biometry and leaf area distribution in an old olive grove at Andria, southern Italy

The objectives of this paper were (1) to provide general biometry data for an 80-year-old olive (Olea europea L., cv. Coratina) grove in Andria, southern Italy, and (2) to compare different methods for estimating leaf area distributions. Stand biometry was represented by a stocking density of 132 trees ha^?1, mean spacing of 8.7 m and mean social area (proportional to spacing and tree size) of about 76 m² per tree. Trunk total circumference averaged 110 cm and after subtraction of missing or dead parts of stems averaged 81 cm, projected area of crowns averaged 17.7 m² and the mean tree height was 4.9 m. Leaf distribution was evaluated using calibrated ground-based side photographs through image analysis and through using a simple canopy-layer model (considering hollow volume within tree crowns) and double-Gaussian curves. The mean leaf size was about 5 cm² (distributed in a log-normal manner over the range of 2 to 12 cm²). Considering whole tree crowns, the mean leaf density was about 2.6 m²m^?3; the maximum leaf area occurred in canopy layers between 1.5 to 3 m, tailing with a steeper slope to the crown base and a less steep slope to the tree-top. The foliated volume of olive crowns (mean 33.2 m³) contained on average 145 thousand leaves of the total area of 72.6 m². The corresponding leaf area index on the stand level (LAI _grove = 0.96), was rather low due to low stocking density. However when taking into account only the projected crown areas (and avoiding free space between trees), the mean LAI reached about 3.5 (range from 1–7). The radial pattern of leaf distribution derived from image analysis indicated peak LAI _rad values at a distance from the stem of about 60 to 70% of crown radius in trees of different size. The applicability of different approaches to the estimation of the necessary allometric parameters is discussed.     

A novel epidemiological model to better understand and predict the observed seasonal spread of Pestivirus in Pyrenean chamois populations

Seasonal variations in individual contacts give rise to a complex interplay between host demography and pathogen transmission. This is particularly true for wild populations, which highly depend on their natural habitat. These seasonal cycles induce variations in pathogen transmission. The seasonality of these biological processes should therefore be considered to better represent and predict pathogen spread. In this study, we sought to better understand how the seasonality of both the demography and social contacts of a mountain ungulate population impacts the spread of a pestivirus within, and the dynamics of, this population. We propose a mathematical model to represent this complex biological system. The pestivirus can be transmitted both horizontally through direct contact and vertically in utero. Vertical transmission leads to abortion or to the birth of persistently infected animals with a short life expectancy. Horizontal transmission involves a complex dynamics because of seasonal variations in contact among sexes and age classes. We performed a sensitivity analysis that identified transmission rates and disease-related mortality as key parameters. We then used data from a long-term demographic and epidemiological survey of the studied population to estimate these mostly unknown epidemiological parameters. Our model adequately represents the system dynamics, observations and model predictions showing similar seasonal patterns. We show that the virus has a significant impact on population dynamics, and that persistently infected animals play a major role in the epidemic dynamics. Modeling the seasonal dynamics allowed us to obtain realistic prediction and to identify key parameters of transmission.

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0218-8) contains supplementary material, which is available to authorized users.     

Using ecological trajectories to track long-term taxonomic and functional changes in benthic shallow soft-bottom communities (Bay of Saint-Brieuc,English Channel)

1. Taxonomic and functional trajectories of benthic assemblages were studied in shallow soft-bottom sediments in the bay of Saint-Brieuc (Western English Channel). Changes were assessed at different spatial and temporal scales using a macrobenthic dataset based on 38 stations sampled in 1987 and 2019, coupled with data from one station sampled annually between 2005 and 2019 as part of the European Water Framework Directive monitoring programme.
2. Taxonomic trajectories indicated 1) changes in the structure and distribution of benthic assemblages, 2) an homogenization of the assemblages, and 3) significant functional shifts.
3. Fishing activities and nutrient enrichment are probably strong drivers of the observed changes, as suggested by the higher mortality rate of fragile, flexible, tubiculous and burrowing species, and the increase in the abundance of opportunistic species in the assemblages. Certain populations of macroinvertebrates seem jointly controlled by climate change and by the aforementioned local factors of disturbance.
4. The Community Trajectory Analysis framework appears as a new and interesting method to track ecological changes in marine ecosystems by measuring change with respect to a baseline state, to help define ecological recovery (station returning to the initial ecological state) and departure (station presenting increased changes over time), and to analyse trajectory similarity.
5. According to the degradation of habitat over time, we identify the need for the implementation of knowledge-based conservation strategies, especially within Natura 2000 sites.

     

犊牛开食料的研制及其犊牛消化试验研究(3)

犊牛开食料是以玉米高梁等谷物类为主,适量加入高能量高蛋白质饲料和促生长剂等组成并对某些关键性原料进行特殊加工处理的植物性固体饲料。经营养成份分析,开食料含粗蛋白质为20.52％,含可消化总养分为76％以上。经36～41d 龄消化试验结果,试验组对日粮各种养分的消化率都接近80％或80％以上,其中粗纤维消化率为53.74％。经85～90d 龄消化试验结果,试验组对各种养分的消化率均高于对照组,除无氮浸出物外,其它养分的消化率均显著高于对照组(P<0.05),特别是对粗纤维的消化率高达63.20％。试验组断奶时共消耗96kg 鲜奶。对照组则消耗416kg 鲜奶。在43～91d 龄期间日粮干物质摄取量对照组为92.47kg,试验组为125.59kg,组间差异极显著(P<0.01)。     

Micro/nano encapsulation via electrified coaxial liquid jets

We report a method to generate steady coaxial jets of immiscible liquids with diameters in the range of micrometer/nanometer size. This compound jet is generated by the action of electro-hydrodynamic (EHD) forces with a diameter that ranges from tens of nanometers to tens of micrometers. The eventual jet breakup results in an aerosol of monodisperse compound droplets with the outer liquid surrounding or encapsulating the inner one. Following this approach, we have produced monodisperse capsules with diameters varying between 10 and 0.15 micrometers, depending on the running parameters.     

Comparative genomics of trypanosomatid parasitic protozoa

A comparison of gene content and genome architecture of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major, three related pathogens with different life cycles and disease pathology, revealed a conserved core proteome of about 6200 genes in large syntenic polycistronic gene clusters. Many species-specific genes, especially large surface antigen families, occur at nonsyntenic chromosome-internal and subtelomeric regions. Retroelements, structural RNAs, and gene family expansion are often associated with syntenic discontinuities that-along with gene divergence, acquisition and loss, and rearrangement within the syntenic regions-have shaped the genomes of each parasite. Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont.     

Degradation pathway of dicyclanil in water in the presence of titanium dioxide. Comparison with photolysis

The solar photolytic behavior of the pesticide 4,6-diamino-2-cyclopropyl-pyrimidine-5-carbonitrile, currently known as dicyclanil, has been mimicked in a photoreactor operating with an artificial light flux. The rate and pathway of degradation were performed. An additional study, using TiO(2) photocatalysis, has been achieved in order to determine the efficiency of photocatalysis to degrade the molecule. The catalyst was titania Degussa P-25. The aim of this article was the identification of the intermediate products formed during the irradiation, to establish the degradation pathway of dicyclanil. The kinetics of the reactions were followed by liquid chromatography with a diode array detector (LC-DAD). Most of the organic compounds occurring during the photodegradation have been identified by means of liquid chromatography and mass spectrometry coupled techniques (HPLC-MS). Additional analyses were carried out to evaluate the mineralization rates into nitrate and ammonium ions.