饲用复合酶对蛋用种鸡日粮磷的利用和生产性能的影响

本试验旨在研究低磷日粮添加复合酶制剂对蛋用种鸡磷的利用和生产性能的影响。选用972只24周龄罗曼褐父母代种鸡,分为3个处理,每处理6个重复,每重复54只种鸡。采用玉米-豆粕-杂粕型基础饲粮,正对照组含总磷0.55%,非植酸磷0.40%,不加酶;负对照组含总磷0.40%,非植酸磷0.21%,不加酶;试验组含总磷0.40%,非植酸磷0.21%,加酶制剂。试验期18周。结果表明:在低非植酸磷的日粮中添加复合酶,粪便中磷含量极显著低于正对照组(P<0.01);粪便中粗蛋白质含量显著低于正对照组和负对照组(P<0.05);显著改善蛋黃顔色(P<0.05);不影响生产性能、骨骼发育和蛋壳质量。添加200 g/t复合酶替代罗曼褐蛋用种鸡日粮中75%的磷酸氢钙能够降低饲料成本,提高养殖企业的经济效益,对罗曼褐蛋用种鸡生产性能无不利影响。     

地理信息系统在森林分类经营中的应用

以黑龙江省大兴安岭林管局新林林业局的森林资源为研究对象,以森林资源的分类经营为研究目标,通过对各种二类调查因子(如海拔、坡度、地类、地被物等)的分析,提出森林分类经营的区划依据,并通过地理信息系统软件ARCVIEW 3.0实现了这一区划,最后提出塔源林场的森林分类经营区划图。     

艾纳香脱氢酶基因家族的生物信息学分析

采用RT-PCR和RACE（Rapid amplification of cDNA ends）技术,从艾纳香（Blumea balsamifera (L.) DC.）叶片中克隆到4条编码艾纳香脱氢酶（BbADH1、BbADH2、BbADH3、BbADH4）基因的cDNA序列,并对4条核苷酸及其编码的氨基酸序列进行生物信息学分析。结果显示:4条艾纳香脱氢酶序列开放阅读框均在900 bp左右,蛋白质等电点（pI）值在5.0~9.0之间,含量最多的氨基酸为亮氨酸（Leu）,最少的为色氨酸（Try）,具有明显的疏水区和亲水区,N端未发现信号肽,且无跨膜区;同源性比对结果显示,艾纳香BbADH蛋白与其他植物中ADH蛋白具有高度的相似性,且具有脱氢酶的特征功能域;系统发育分析表明,艾纳香（B. balsamifera (L.) DC.）BbADH1和BbADH3同处于一个分支,且与胡杨（Populus euphratica Oliv.）PeADH物种亲缘关系较近,而艾纳香BbADH4和BbADH2处于不同分支,且分别与葡萄（Vitis vinifera）VvADH和烟草（Nicotiana tabacum）NtADH物种亲缘关系较近。     

Mechanism of elemene enhancing radiosensitivity of human glioma U251 cells

AIM To investigate the effect of elemene on the radiosensitivity of human glioma U251 cells and its mechanism. METHODS The U251 cells were used as a glioma model in vitro, and were exposed to different concentrations of elemene and different doses of radiation. The cell viability was measured by MTT assay, the apoptosis and cell cycle distribution were analyzed by flow cytometry, and the related protein levels were determined by Western blot. RESULTS Elemene inhibited the viability of U251 cells in vitro and enhanced the radiosensitivity of the cells. The cells in radiotherapy combined with elemene group had higher rates of early apoptosis, secondary necrosis and total cell death than those in radiation group. Elemene induced G₂/M phase arrest in the U251 cells. Elemene reduced the protein expression of cell division cycle protein 2 （Cdc2）, which resulted in the decrease in cyclin B1 expression induced by radiotherapy, thereby inhibiting the formation of cyclin B-Cdc2 complex. Elemene reduced Cdc2 activity by inhibiting the phosphorylation of Cdc2 protein at threonine 161, thereby inducing G₂/M phase arrest in the cells. It also mediated apoptosis by down-regulating survivin expression. CONCLUSION Elemene may increase the sensitivity of U251 cells to radiotherapy by down-regulating Cdc2 protein, decreasing cyclin B1 expression, inhibiting the formation of cylcin B-Cdc2 complex and down-regulating the expression of survivin.     

地貌发育阶段判识方法研究——以泥河沟流域和枣子沟流域为例

为更加准确判识小流域地貌发育阶段,以黄土高原综合治理小流域——陕西省淳化县泥河沟流域和乾县枣子沟流域为例,分别应用盒分维方法和Horton分维方法进行水系分维值的计算,并利用面积高程积分和地貌信息熵进行验证。结果表明,泥河沟和枣子沟流域水系盒分维值分别为1.886和1.827。根据Strahler曲线积分值和地貌信息熵方法验证结果表明,盒分维方法计算的水系分维值对流域地貌发育阶段的判识更加准确、可靠;这2个流域地貌处于侵蚀发育阶段的壮龄期,其生态敏感性整体上处于较低水平,生态环境抗干扰能力增强并趋于更加稳定的状态。     

我国农村土地流转研究综述

基于CNKI核心期刊及CSSCI数据库,对2009～2019年有关农地流转的研究文献进行了回顾,梳理了我国农地流转研究成果,对农地流转的研究主要从农户农地流转决策影响因素、农地流转对农民收入影响、农地流转过程中存在问题及对策等方向进行。运用系统分析法对我国农地流转的研究成果进行分析,探究农地流转的内在规律和发展趋势,为规范有序推进农地流转,深化农村土地制度改革和优化乡村振兴路径提供参考与借鉴。     

杂种落叶松的识别技术

杂种落叶松在抗早期落叶病、抗寒性等方面具有一定优势,冻害指数较日本落叶松低70%,早期落叶病发病率较兴安落叶松低15%、感病指数下降15%。根据针叶形态和一些生理生化手段可将杂种落叶松和亲本区分开来。     

白洋淀流域水环境风险评估综述

以白洋淀流域水污染问题为研究对象,对其水质现状、风险源和风险因子等方面进行总结和分析,并运用层次分析法初步构建了白洋淀流域水环境风险评估体系框架.最后,基于已有白洋淀流域水环境污染研究进展,提出以后应进一步加强的地方:①加强白洋淀流域水环境综合风险评估研究,并使用数学模型来综合评判白洋淀流域水环境风险水平;②加强风险预...