全文获取类型
收费全文 | 208篇 |
免费 | 14篇 |
专业分类
林业 | 80篇 |
农学 | 2篇 |
23篇 | |
综合类 | 20篇 |
农作物 | 7篇 |
水产渔业 | 23篇 |
畜牧兽医 | 61篇 |
园艺 | 1篇 |
植物保护 | 5篇 |
出版年
2021年 | 5篇 |
2020年 | 5篇 |
2019年 | 1篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 7篇 |
2015年 | 6篇 |
2014年 | 3篇 |
2013年 | 19篇 |
2012年 | 10篇 |
2011年 | 10篇 |
2010年 | 8篇 |
2009年 | 4篇 |
2008年 | 11篇 |
2007年 | 9篇 |
2006年 | 11篇 |
2005年 | 8篇 |
2004年 | 8篇 |
2003年 | 12篇 |
2002年 | 10篇 |
2001年 | 10篇 |
2000年 | 9篇 |
1999年 | 9篇 |
1998年 | 8篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1972年 | 1篇 |
1968年 | 1篇 |
1967年 | 2篇 |
排序方式: 共有222条查询结果,搜索用时 15 毫秒
81.
Yamamoto Y Fukushima T Suna Y Ishii N Saeki A Seki S Tagawa S Taniguchi M Kawai T Aida T 《Science (New York, N.Y.)》2006,314(5806):1761-1764
Controlled self-assembly of a trinitrofluorenone-appended gemini-shaped amphiphilic hexabenzocoronene selectively formed nanotubes or microfibers with different photochemical properties. In these nanotubes, which are 16 nanometers in diameter and several micrometers long, a molecular layer of electron-accepting trinitrofluorenone laminates an electron-donating graphitic layer of pi-stacked hexabenzocoronene. The coaxial nanotubular structure allows photochemical generation of spatially separated charge carriers and a quick photoconductive response with a large on/off ratio greater than 10(4). In sharp contrast, the microfibers consist of a charge-transfer complex between the hexabenzocoronene and trinitrofluorenone parts and exhibit almost no photocurrent generation. 相似文献
82.
Keisuke Kojiro Yuzo Furuta Makoto Ohkoshi Yutaka Ishimaru Misao Yokoyama Junji Sugiyama Shuichi Kawai Takumi Mitsutani Hiromasa Ozaki Minoru Sakamoto Mineo Imamura 《Journal of Wood Science》2008,54(6):515-519
To investigate the changes in microstructures of wood with elapsed time in the environment, CO2 adsorption onto dry wood was measured at ice-water temperature (273 K) for samples aged from 0.1 years to over 1000 years.
The micropore size distribution was obtained using the Horvath-Kawazoe method. Micropores smaller than 0.6 nm in wood decreased
in number with elapsed time in the environment, and a negative correlation was found between cumulative pore volume for pores
smaller than 0.6 nm and elapsed time in the environment. Cumulative pore volume in the 1000-year sample was almost half of
that in the 0.1- year sample. Micropores smaller than 0.6 nm in wood with a few decades or more of elapsed time increased
in number after rewetting and drying. Consequently, microstructures of wood with longer time elapsed in the environment were
considered to be more stable, because of longer-term thermal motion and possibly more repeated moisture adsorption and desorption
and/or temperature variation in the environment. 相似文献
83.
Control of Pseudomonas mastitis on a large dairy farm by using slightly acidic electrolyzed water
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Kazuhiro Kawai Yasunori Shinozuka Ikuo Uchida Kazuhiko Hirose Takashi Mitamura Aiko Watanabe Kana Kuruhara Reiko Yuasa Reiichiro Sato Ken Onda Hajime Nagahata 《Animal Science Journal》2017,88(10):1601-1605
The disinfection effect of slightly acidic electrolyzed water (SAEW) use in a farm where Pseudomonas mastitis has spread was evaluated. Despite the application of antibiotic therapy and complete cessation of milking infected quarters, numerous new and recurrent Pseudomonas aeruginosa clinical mastitis infections (5.8–7.1% of clinical mastitis cases) occurred on the farm from 2003 to 2005. Procedural changes and equipment modifications did not improve environmental contamination or the incidence of Pseudomonas mastitis. To more thoroughly decontaminate the milking parlor, an SAEW system was installed in 2006. All milking equipment and the parlor environment were sterilized with SAEW (pH 5–6.5, available chlorine 12 parts per million) before and during milking time. After adopting the SAEW system, the incidence of clinical and subclinical Pseudomonas mastitis cases decreased significantly (P < 0.0001) and disappeared. These findings suggest that SAEW effectively reduced the incidence of mastitis in a herd contaminated by Pseudomonas species. This is the first report to demonstrate the effectiveness of disinfection by SAEW against mastitis pathogens in the environment. 相似文献
84.
Growth of Salmonella typhimurium and Salmonella enteritidis in egg yolks from highly immunized hens.
K Takase T Nakayama T Kawai H Fujikawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(8):959-960
This experiment was conducted to ascertain whether the growth of Salmonella Enteritidis (SE) or Salmonella Typhimurium (ST) would be suppressed in the presence of antibodies contained in egg yolks. Specific pathogen-free chickens (102 days of age) were subcutaneously immunized with oil-adjuvanted bacterin of SE or ST, twice within a four-week interval. During 160 to 170 days of age, eggs were collected, the yolks were removed and mixed with an equal volume of physiological buffered saline, inoculated with ten colony forming units (CFU) of SE or ST, and incubated at 37 degrees C or 20 degrees C for 23 hr. The growth of organisms in each yolk solution was examined. The egg yolk derived from non-immunized hens was examined in the same manner as the controls. There was no difference in the growth titer between the antibody-positive yolk and the negative yolk. The result suggests that the antibodies in the yolk do not influence the growth of each organism, even if the hens are highly immunized. 相似文献
85.
JDA Losano DSR Angrimani A Dalmazzo BR Rui MM Brito CM Mendes GKV Kawai CI Vannucchi MEOA Assumpção VH Barnabe M Nichi 《Reproduction in domestic animals》2017,52(2):289-297
Studies have demonstrated the importance of mitochondria to sperm functionality, as the main source of ATP for cellular homoeostasis and motility. However, the role of mitochondria on sperm metabolism is still controversial. Studies indicate that, for some species, glycolysis may be the main mechanism for sperm energy production. For ram sperm, such pathway is not clear. Thus, we evaluated ram sperm in response to mitochondrial uncoupling and glycolysis inhibition aiming to assess the importance of each pathway for sperm functionality. Statistical analysis was performed by the SAS System for Windows, using the General Linear Model Procedure. Data were tested for residue normality and variance homogeneity. A p < .05 was considered significant. Groups treated with the mitochondrial uncoupler Carbonyl cyanide 3 chlorophenylhydrazone (CCCP) showed a decrease in the percentage of cells with low mitochondrial activity and high mitochondrial membrane potential. We also observed that the highest CCCP concentration promotes a decrease in sperm susceptibility to lipid peroxidation. Regardless the lack of effect of CCCP on total motility, this substance induced significant alterations on sperm kinetics. Besides the interference of CCCP on spermatic movement patterns, it was also possible to observe such an effect in samples treated with the inhibitor of glycolysis (2‐deoxy‐d ‐glucose, DOG). Furthermore, treatment with DOG also led to a dose‐dependent increase in sperm susceptibility to lipid peroxidation. Based on our results, we suggest that the glycolysis appears to be as important as oxidative phosphorylation for ovine sperm kinetics as this mechanism is capable of maintaining full motility when most of the cells have a low mitochondrial membrane potential. Furthermore, we found that changes in the glycolytic pathway trough glycolysis inhibition are likely involved in mitochondrial dysfunction and sperm oxidative unbalance. 相似文献
86.
N Sasaki K Takahashi S Kawamoto T Kurosawa M Sonoda S Kawai 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(3):495-501
The parthenogenetic Haemaphysalis longicornis larvae engorged on cattle naturally infected with Theileria sergenti were reared at 24 degrees C. The resultant nymphal ticks were incubated at 37 degrees C to clear the effect of incubation on the development and maturation of sporozoites. The sporozoites in the salivary glands of the nymphal ticks exposed to 37 degrees C for 16 days were observed by the methyl green pyronin staining method. The ticks exposed to 37 degrees C were ground up in a mortar and the supernatant of the tick suspension in PBS was inoculated into cattle. The cattle showed parasitemia and specific antibody response 18 days after inoculation. Consequently, the parasites in the tick salivary glands became infective to cattle by incubating infected. H. longicornis nymphs at 37 degrees C. 相似文献
87.
88.
Collagenofibrotic glomerulonephropathy (CFGN) is characterized by the deposition of type III collagen within the mesangial matrix and the absence of mesangial cell proliferation. A case of CFGN in a 2.7-year-old female cynomolgus macaque was investigated in the present study. Clinically, the animal was shown to have severe systemic edema along with hypoproteinemia. At necropsy, the kidneys were swollen and pale. The glomerular lesions were characterized by massive diffuse and global accumulation of fibrous materials in the mesangial areas. Neither mesangial cell proliferation nor changes in other organs were found. The fibrous materials were confirmed by the results of immunohistochemical and electron microscopic findings to consist mainly of randomly arranged, curve-shaped, twisted, and entwined type III collagen. This is the first case report of CFGN in nonhuman primates to date. 相似文献
89.
S. M. M. Nakayama Y. Ikenaka A. Hayami H. Mizukawa W. S. Darwish K. P. Watanabe Y. K. Kawai M. Ishizuka 《Journal of veterinary pharmacology and therapeutics》2016,39(5):478-487
Research on drug metabolism and pharmacokinetics in large animal species including the horse is scarce because of the challenges in conducting in vivo studies. The metabolic reactions catalyzed by cytochrome P450s (CYPs) are central to drug pharmacokinetics. This study elucidated the characteristics of equine CYPs using diazepam (DZP) as a model compound as this drug is widely used as an anesthetic and sedative in horses, and is principally metabolized by CYPs. Diazepam metabolic activities were measured in vitro using horse and rat liver microsomes to clarify the species differences in enzyme kinetic parameters of each metabolite (temazepam [TMZ], nordiazepam [NDZ], p‐hydroxydiazepam [p‐OH‐DZP], and oxazepam [OXZ]). In both species microsomes, TMZ was the major metabolite, but the formation rate of p‐OH‐DZP was significantly less in the horse. Inhibition assays with a CYP‐specific inhibitors and antibody suggested that CYP3A was the main enzyme responsible for DZP metabolism in horse. Four recombinant equine CYP3A isoforms expressed in Cos‐7 cells showed that CYP3A96, CYP3A94, and CYP3A89 were important for TMZ formation, whereas CYP3A97 exhibited more limited activity. Phylogenetic analysis suggested diversification of CYP3As in each mammalian order. Further study is needed to elucidate functional characteristics of each equine CYP3A isoform for effective use of diazepam in horses. 相似文献
90.
The phenylpropanoid glycosides, vanicoside A and B, isolated from rhizomes of giant knotweed (Polygonum sachalinense) showed beta-glucosidase inhibitory activity, with IC(50) values of 59.8 and 48.3 mug/ml (59.9 and 50.5 muM), respectively. In contrast, p-coumaric acid and ferulic acid, corresponding to phenylpropanoyl moieties of vanicosides, exhibited very little inhibition. 相似文献