全文获取类型
收费全文 | 318篇 |
免费 | 11篇 |
专业分类
林业 | 15篇 |
农学 | 17篇 |
26篇 | |
综合类 | 19篇 |
农作物 | 27篇 |
水产渔业 | 59篇 |
畜牧兽医 | 122篇 |
园艺 | 5篇 |
植物保护 | 39篇 |
出版年
2024年 | 1篇 |
2022年 | 6篇 |
2021年 | 8篇 |
2020年 | 6篇 |
2019年 | 3篇 |
2018年 | 8篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 14篇 |
2014年 | 21篇 |
2013年 | 21篇 |
2012年 | 21篇 |
2011年 | 21篇 |
2010年 | 12篇 |
2009年 | 8篇 |
2008年 | 15篇 |
2007年 | 24篇 |
2006年 | 23篇 |
2005年 | 21篇 |
2004年 | 13篇 |
2003年 | 27篇 |
2002年 | 11篇 |
2001年 | 6篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1980年 | 1篇 |
排序方式: 共有329条查询结果,搜索用时 15 毫秒
91.
Partial sequences of the 18S rRNA gene (625 bp) from a Hepatozoon detected in two canine hepatozoonosis cases, one clinical and one subclinical, in Japan were analyzed. Both sequences were identical to each other and they were closely related to the Hepatozoon canis strain found in Israel with 99% (617/625) nucleotide identity. Both Hepatozoon americanum and Hepatozoon catasbianae were distantly related to the Japanese Hepatozoon with 94% (586/625) and 91% (566/625) identities, respectively. In a phylogenetic tree, the Japanese Hepatozoon was most closely related to H. canis from Israel but was significantly different than H. americanum and H. catasbianae. These results suggest that the Hepatozoon detected in the Japanese dogs might be a strain variant of H. canis, but is apparently a different species than H. americanum. 相似文献
92.
Arai T Ogawa T Nakamura M Hosoya M Ohnishi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1065-1067
Activities of malate dehydrogenase (MDH) and aspartate aminotransferase in the malate-aspartate shuttle were significantly increased in the cytosolic fractions of livers with early neoplastic symptoms such as swelling and discoloration in transgenic mice carrying the prototype human c-Ha-ras gene, Tg-rasH2 mice, which were administered with diethylnitrosamine (DEN) as a carcinogenic chemical at a dose of 200 mg/kg body weight. Cytosolic MDH/LDH (lactate dehydrogenase) (ML) ratio increased significantly and was considered to be a useful marker to characterize the energy metabolism at early neoplastic stage in livers of the Tg-rasH2 mice. 相似文献
93.
Koji Tanaka Sayaka Nakatsu Zen-nosuke Katsuba Hiroshi Furuya Takao Tsuchiya Takashi Oku 《Journal of General Plant Pathology》2005,71(2):144-146
The pathogenic races of 450 cultures of Xanthomonas oryzae pv. oryzae, isolated from eight locations in the inland mountainous area of Hiroshima Prefecture during 2000 to 2003, were determined with a set of Japanese differentials. The rice cultivars infected with the bacterium are thought to be in the Kinmaze group, which does not have any resistance genes. Five Japanese races IA, IB, II, V, and VII occurred across the area, although the composition of the races in each location altered during the surveyed 4 years. 相似文献
94.
Naruto FURUYA Hiroyuki URA Kazuhiro IIYAMA Masaru MATSUMOTO Minoru TAKESHITA Yoichi TAKANAMI 《Journal of General Plant Pathology》2002,68(3):220-224
Specific primers were designed based on the sequences of the spacer region between the 16S and 23S ribosomal DNA (rDNA) for
direct, rapid and specific detection of Burkholderia gladioli. These primers were named GLA-f and GLA-r. PCR performed on boiled bacterial suspensions yielded an amplification product of
approximately 300 bp. No products from other bacterial species, including B. glumae were amplified, even after complete DNA extraction by the cetyltrimethyl-ammonium bromide (CTAB) method. Using the specific
primers designed in this study, the PCR method can detect B. gladioli in plant samples within 6 hr. These data demonstrate the potential of specific PCR for the detection of B. gladioli.
Received 10 December 2001/ Accepted in revised form 15 April 2002 相似文献
95.
Inokuma H Nagata M Hosoi E Itamoto K Okuda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(4):401-405
The 18S rRNA gene and the piroplasm major immunodominant protein gene (p33/34) of Theileria from various subspecies of sika deer in 8 different locations of Japan were analyzed. The similarity between 633 bp partial sequences of the 18S rRNA gene among various subspecies of sika deer was found to be between 99.7% and 100%. While the percent identities of the 412 bp partial p33/34 gene sequence and deduced amino acid sequences between Theileria of sika deer from Yamaguchi Prefecture and those found in deer from other Prefectures, were comparatively low, 68.7% to 70.1% and 64.1% to 70.0% respectively. These findings suggest that there are at least two genetically distinct strains of Theileria of sika deer in Japan. 相似文献
96.
Yoshinori Yamanaka Masaru Moritomo Katsutoshi Fujii Toshinobu Tanaka Yasuhisa Fukuda Keiichiro Nishimura 《Pest management science》1999,55(9):896-902
A set of N-(4-difluoromethoxybenzyl)pyrimidinamines with various substituents at the 4- and 5-positions of the pyrimidine ring and at the benzyl position were prepared, and their fungicidal activities against wheat brown rust, Puccinia recondita, and barley powdery mildew, Erisiphe graminis, were measured. Variations in each of these activities were quantitatively analysed by the use of physicochemical substituent parameters and a regression analysis. Each of these activities was parabolically correlated with the steric bulkiness of the pyrimidine substituents and with the bulkiness or the hydrophobicity of the benzylic substituents. © 1999 Society of Chemical Industry 相似文献
97.
Journal of Plant Diseases and Protection - Phomopsis asparagi has been reported as a serious disease, causing stem blight on asparagus cultivars (Asparagus officinalis), and this fungus was also... 相似文献
98.
Jun Shoji Tsutomu Maehara Mitsuhiro Aoyama Hiroshi Fujimoto Akio Iwamoto Masaru Tanaka 《Fisheries Science》2001,67(2):238-245
SUMMARY: Diel successive samplings of Japanese Spanish mackerel Scomberomorus niphonius larvae were conducted throughout 24 h both in the sea and in captivity in order to estimate their daily ration. Using the Elliott and Persson model, the instantaneous gastric evacuation rate was estimated from the depletion of stomach contents (% dry bodyweight) with time during the night for wild fish (3.0–11.5 mm standard length) and from starvation experiments for reared fish (8, 10, and 15 days after hatching (DAH)). Japanese Spanish mackerel is a daylight feeder and exhibited piscivorous habits from first feeding both in the sea and in captivity. Feeding activity peaked at dusk. The estimated daily ration for wild larvae were 111.1 and 127.2% in 1996 and 1997, respectively; and those for reared larvae ranged from 90.6 to 111.7% of dry bodyweight. Based on the estimated value of daily rations for reared fish, the total number of newly hatched red sea bream Pagrus major larvae preyed by a Japanese Spanish mackerel from first feeding (5 DAH) to beginning of juvenile stage (20 DAH) in captivity was calculated to be 1139–1404. 相似文献
99.
Changes in quantitative profile of extracellular matrix components in the kidneys of rats with adriamycin-induced nephropathy 总被引:1,自引:0,他引:1
Manabe N Kinoshita A Yamaguchi M Furuya Y Nagano N Yamada-Uchio K Akashi N Miyamoto-Kuramitsu K Miyamoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(2):125-133
Extracellular matrix components (ECMs) in histological sections of the kidney cortex from the rats with adriamycin (ADR)-induced nephropathy (5 mg/kg, i.v.) were quantified by an immunohistochemical micromethod. Changes in kidney histopathology and urine and blood biochemistry were investigated. Enlarged kidneys were granular on the surface and pale in color in ADR-treated rats, and these rats had kidneys with glomeruli with expanded mesangial area and with capillary aneurysm. Severe albuminuria, hypoalbuminemia, hypercholesterolemia and disorders in other nephrotic parameters were observed in ADR-treated rats. Type I and IV collagens, fibronectin and laminin contents in the renal cortex of ADR-treated rats at 10 weeks were 329, 317, 263 and 295%, respectively, higher than in each vehicle control, and those at 28 weeks were 1,211, 930, 1,057 and 1,012%, respectively. The glomerular sclerotic abnormalities progressed in a time-dependent manner. Moreover, there was a strong correlation between the ECM levels and serum creatinine and blood urea nitrogen levels. In conclusion, microquantification provided useful information for accurate diagnosis and prognosis of nephrotic lesions and is a good tool to assess the advancement of renal disorders in patients with nephropathy. 相似文献
100.
Okamura Y Haraguchi T Morimoto M Okuda M Une S Nakaichi M Taura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(6):651-658
Receptor-binding cancer antigen expressed on SiSo cells (RCAS1), one of novel cancer cell-surface antigens, is strongly expressed in invasive cancers. RCAS1 inhibits the in vitro growth of lymphocytes such as T cells and natural killer (NK) cells, and induces apoptotic cell death. We investigated the expression of RCAS1 in canine mammary tumor cell lines and tumor cells by immunohistochemistry, and also in situ deoxyribonucleic acid (DNA) fragmentation in tumor-infiltrating lymphocytes (TILs) by the terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. All canine mammary tumor cell lines expressed RCAS1 at both the messenger ribonucleic acid (mRNA) and protein level. Immunohistochemically, RCAS1 was negative in 100% of normal mammary glands, but was expressed in 100% of malignant tumors examined. In most malignant mammary tumors, RCAS1 was localized in the cytoplasm with no polarity of expression. In benign mammary tumors, it was detected on the luminal surface of the tumor cell. RCAS1 expression or localization was significantly correlated with malignancy. In situ DNA fragmentation of CD3-positive TILs was observed in RCAS1-expressing tumors. RCAS1-expressing tumors, indicating a possible induction of apoptotic cell death in TILs through RCAS1 expression. These observations suggest that RCAS1 probably plays an important role in tumor progression and escape from immune surveillance in canine mammary tumors. 相似文献