Ripening of Tomatoes

Experiments were carried out with aldrin, dieldrin, isodrin, endrin and DDT to ascertain whether, employed as insecticides, these compounds had any effect on cell division in onion root-tips. The doses used were equal to, or greatly in excess of, the recommended rates of application. By germinating onion seeds in direct contact with the insecticide under investigation, it has been shown that none of the compounds had any effects on germination or growth rate. High doses of aldrin, dieldrin or isodrin had a slight toxic effect on the resting cell, which, with aldrin, was also caused by the recrystallized compound. Aldrin and dieldrin had no effect on the dividing cell. Isodrin and endrin caused slight stickiness between chromatids in anaphase, which was insufficient to prevent the completion of division in the cell, and DDT caused a delay in the early stages of prophase and a shortening of chromosomes in metaphase.

Pot experiments, with high doses of each compound, showed that germination and growth rate were unaffected by any of the compounds. Resting cells were not affected and dividing cells were affected only by slight stickiness in anaphase, except with DDT, which also caused a slight delay in metaphase.

Attention has been drawn to the advantages of these compounds over gamma-BHC, chlordane and toxaphene as insecticides, from the point of view of causing less damage to the plant.     

Kinetic study of the thermal hydrolysis of Agave salmiana for mezcal production

The kinetics of the thermal hydrolysis of the fructans of Agave salmiana were determined during the cooking step of mezcal production in a pilot autoclave. Thermal hydrolysis was achieved at different temperatures and cooking times, ranging from 96 to 116 °C and from 20 to 80 h. A simple kinetic model of the depolymerization of fructans to monomers and other reducing sugars and of the degradation of reducing sugars to furans [principally 5-(hydroxymethyl)furfural, HMF] was developed. From this model, the rate constants of the reactions were calculated, as well as the pre-exponential factors and activation energies of the Arrhenius equation. The model was found to fit the experimental data well. The tradeoff between a maximum fructan hydrolysis and a critical furan concentration in allowing for the best ethanol yield during fermentation was investigated. The results indicated that the thermal hydrolysis of agave was optimal, from the point of view of ethanol yield in the ensuing fermentation, in the temperature range of 106-116 °C and the cooking range time of 6-14 h. The optimal conditions corresponded to a fructan hydrolysis of 80%, producing syrups with furan and reducing sugar concentrations of 1 ± 0.1 and 110 ± 10 g/L, respectively.     

The role of calcium in the mechanical performance of cattle ruminal muscle

A viable preparation from the external muscle coat of cattle rumen for in vitro studies of contractility is described. The threshold Ca²⁺ concentration for contractile response in glycerol treated ruminal muscle was found to be pH dependent. At physiological pH it is situated between 10^-8 and 10^-7 M. Strips with intact membranes were studied in the depolarized state. In this preparation contraction to acetylcholine or histamine requires Ca²⁺ in the medium. However, contractility persists for several minutes in Ca²⁺-free solution at rest but disappears rapidly during stimulation. Recovery in a Ca²⁺-containing medium is much faster than the decline of responsiveness in a Ca²⁺-free medium. Tetracaine and D600 seem to inhibit contraction by blocking release of Ca²⁺ from and uptake of Ca²⁺ into hypothetical Ca stores inside the cell. The results are interpreted by assuming cellular Ca stores and two Ca pumps, one extruding Ca²⁺ into the medium and one accumulating Ca in the stores. Acetylcholine and histamine act by increasing Ca²⁺ permeability of both the membranes of the stores and the plasma membrane. The stimulator-induced and possibly the resting Ca²⁺ permeability in the depolarized state is reduced by tetracaine and D600 at both sites. The pumps are assumed not to be affected by stimulators and the mentioned drugs.     

Equine ehrlichial colitis (Potomac horse fever): recognition of the disease in Pennsylvania, New Jersey, New York, Ohio, Idaho, and Connecticut

Equine ehrlichial colitis (Potomac horse fever), a newly identified colitis of the horse, was first recognized in Maryland. In this report, we document occurrence of the disease in Pennsylvania, New Jersey, New York, Ohio, Idaho, and Connecticut. Enzootic areas were recognized by a characteristic pattern. Frequently there was a seasonal pattern and high prevalence of sporadic colitis in unstressed horses. The attack rate per farm generally was low. Horses on pasture, as well as those stabled, were affected. Clinical signs varied from fever and depression to severe diarrhea and laminitis. Occasionally horses developed profound ileus and severe colic. Diagnosis was based on detection of an increase or decrease in serum antibody titers to Ehrlichia risticii, using an indirect fluorescent antibody technique.