Purine resistant mutants of Drosophila are adenine phosphoribosyltransferase deficient

A deficiency for adenine phosphoribosyltransferase activity is the primary biochemical defect in mutants of Drosophila selected for resistance to purine-induced lethality.     

Analysis of phenolic compounds by high-performance liquid chromatography and liquid chromatography/mass spectrometry in potato plant flowers, leaves, stems, and tubers and in home-processed potatoes

Potato plants synthesize phenolic compounds as protection against bruising and injury from bacteria, fungi, viruses, and insects. Because antioxidative phenolic compounds are also reported to participate in enzymatic browning reactions and to exhibit health-promoting effects in humans, a need exists for accurate methods to measure their content in fresh and processed potatoes. To contribute to our knowledge about the levels of phenolic compounds in potatoes, we validated and used high-performance liquid chromatography and liquid chromatography/mass spectrometry to measure levels of chlorogenic acid, a chlorogenic isomer, and caffeic acid in flowers, leaves, stems, and tubers of the potato plant and in home-processed potatoes. The total phenolic acid content of flowers (626 mg/100 g fresh wt) was 21 and 59 times greater than that of leaves and stems, respectively. For all samples, chlorogenic acid and its isomer contributed 96-98% to the total. Total phenolic acid levels (in g/100 g fresh wt) of peels of five potato varieties grown in Korea ranged from 6.5 to 42.1 and of the flesh (pulp) from 0.5 to 16.5, with peel/pulp ratios ranging from 2.6 to 21.1. The total phenolic acid content for 25 American potatoes ranged from 1.0 to 172. The highest amounts were present in red and purple potatoes. Home processing of pulp with various forms of heat induced reductions in the phenolic content. The described methodology should facilitate future studies on the role of potato phenolic compounds in the plant and the diet.     

Potato glycoalkaloids and metabolites: roles in the plant and in the diet

Potatoes, members of the Solanaceae plant family, serve as major, inexpensive low-fat food sources providing energy (starch), high-quality protein, fiber, and vitamins. Potatoes also produce biologically active secondary metabolites, which may have both adverse and beneficial effects in the diet. These include glycoalkaloids, calystegine alkaloids, protease inhibitors, lectins, phenolic compounds, and chlorophyll. Because glycoalkaloids are reported to be involved in host-plant resistance and to have a variety of adverse as well as beneficial effects in cells, animals, and humans, a need exists to develop a clearer understanding of their roles both in the plant and in the diet. To contribute to this effort, this integrated review presents data on the (a) history of glycoalkaloids; (b) glycoalkaloid content in different parts of the potato plant, in processed potato products, and in wild, transgenic, and organic potatoes; (c) biosynthesis, inheritance, plant molecular biology, and glycoalkaloid-plant phytopathogen relationships; (d) dietary significance with special focus on the chemistry, analysis, and nutritional quality of low-glycoalkaloid potato protein; (e) pharmacology and toxicology of the potato glycoalkaloids comprising alpha-chaconine and alpha-solanine and their hydrolysis products (metabolites); (f) anticarcinogenic and other beneficial effects; and (g) possible dietary consequences of concurrent consumption of glycoalkaloids and other biologically active compounds present in fresh and processed potatoes. An enhanced understanding of the multiple and overlapping aspects of glycoalkaloids in the plant and in the diet will benefit producers and consumers of potatoes.     

A CASE ASSESSMENT OF THE MECHANISMS INVOLVED IN HUMAN‐INDUCED LAND DEGRADATION IN NORTHEASTERN KUWAIT

Soil degradation and vegetation deterioration in contrasting areas (open desert versus protected desert) in the northeastern part of Kuwait were investigated, using field measurements, and laboratory methodologies. The average infiltration rate of compacted soils studied was found to be 52 per cent lower than that of the undisturbed soils we investigated. The bulk density of the compacted soils was 19 per cent higher than that of the non‐compacted soils. The average topsoil resistance of severely compacted soils was 13 per cent greater than that of undisturbed soils and is mainly due to overgrazing, and off‐road vehicle use. We found that the overall vegetation cover status of open areas investigated was approximately 70 per cent less than for the protected areas studied. As a consequence of soil compaction and vegetation deterioration, sand deflation processes, and sand movement prevail in open areas. The total average percentages of course grain sizes in unprotected soils and heavily disturbed soils by vehicles (off‐road) are 51 and 103 per cent greater, respectively. The annual rate of sand transport during the last 20 years, from the prevailing wind direction (NW) in the area has increased by 81 per cent. The delicate balance between soil and natural vegetation cover is easily disturbed by off‐road vehicle use associated with overgrazing and recreation activity. A restoration plan is needed in order to reduce land degradation and to allow natural vegetation recovery. Copyright © 2011 John Wiley & Sons, Ltd.