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71.
Boleti AP Freire Md Coelho MB Silva Wd Baldasso PA Gomes VM Marangoni S Novello JC Macedo ML 《Journal of agricultural and food chemistry》2007,55(7):2653-2658
This paper describes the purification and characterization of a novel protein from the seeds of Pouteria torta (family Sapotaceae). The protein was purified by a combination of gel filtration, ion-exchange, and reverse phase chromatographies. SDS-PAGE of the purified protein resulted in a single protein band of 14 kDa in the presence and absence of DTT. The lectin-like activity of pouterin was best inhibited by glycoproteins such as fetuin, asialofetuin, heparin, orosomucoid, and ovoalbumin. Pouterin inhibited the growth of the fungi Fusarium oxysporum and Colletotrichum musae and of the yeast Saccharomyces cerevisiae. The incorporation of pouterin into an artificial diet (final concentration = 0.12%, w/w) caused 50% mortality in larvae of the insect Callosobruchus maculatus, whereas 0.08% pouterin produced an ED50. 相似文献
72.
Ceron F. Juliane M. Prodocimo Viviane Freire Carolina A. 《Fish physiology and biochemistry》2021,47(4):1063-1071
Fish Physiology and Biochemistry - Ray-finned fishes of the superorder Ostariophysi are primarily freshwater (FW), and normally stenohaline. Differently, fishes of the superorder Acanthopterygii... 相似文献
73.
Macedo ML Freire Md Martins LT Martinez DS Gomes VM Smolka MB Toyama MH Marangoni S Coelho LC 《Journal of agricultural and food chemistry》2004,52(25):7548-7554
This study starts by isolating and characterizing the first protein from Labramia bojeri seeds, which belong to the Sapotaceae family. The purified lectin analyzed by SDS-PAGE with and without beta-mercaptoethanol shows two protein bands (M(r) = 19 and 20 kDa), which cannot be resolved. Protein bands have shown similar characteristics as molecular masses, determined by gel filtration and native gel; N-terminal sequences presented a difference in their isoelectric points. We have suggested that those protein bands might be variants of the protein named Labramin. The sequence database search has shown that the N-terminal sequence of Labramin presented a high degree of homology to Kunitz-type trypsin inhibitor (82-52%) despite no trypsin inhibition activity detection. The lectin-like form from Labramin was better inhibited by glycoproteins and has also presented growth inhibition of the fungus Colletotrichum lindemuthianum and the yeast Saccharomyces cerevisiae, but it has not presented an apparent effect on Fusarium oxysporum. 相似文献
74.
Andre Freire Cruz Bruno Ferreira de Oliveira Marcio de Carvalho Pires 《International Journal of Fruit Science》2017,17(3):259-268
An experiment under greenhouse conditions was carried out to evaluate the best level of nitrogen (N) and phosphorus (P), which promote the growth of papaya (Carica papaya L. var. Solo) seedlings without affecting the arbuscular mycorrhizal fungi (AMF) activity. Seedlings of papaya were transplanted to pots inoculated or not with Gigaspora margarita spores at four levels of N (0, 50, 100, and 200 ppm) and P (0, 25, 50, and 100 ppm). P was amended at the same time of pot filling up with substrate but N was applied weekly. The experiment was harvested after 100 days and plant dry weight together with AMF colonization was measured. The major response on the plant dry weight and height was from the fertilizers (N and P) where it proportionally increased. For the dry weight, at low amounts of nutrients the effects of AMF were greater, but as much as the fertilizer level in soil increased this response decreased. The colonization decreased as the nutrient levels increased, especially the P. The best combination of N and P for plant growth and AMF colonization could be at 50 and 25 ppm, equivalent to 5 and 2.5 kg/10a, respectively. 相似文献
75.
Champion DJ Ransom SM Lazarus P Camilo F Bassa C Kaspi VM Nice DJ Freire PC Stairs IH van Leeuwen J Stappers BW Cordes JM Hessels JW Lorimer DR Arzoumanian Z Backer DC Bhat ND Chatterjee S Cognard I Deneva JS Faucher-Giguère CA Gaensler BM Han J Jenet FA Kasian L Kondratiev VI Kramer M Lazio J McLaughlin MA Venkataraman A Vlemmings W 《Science (New York, N.Y.)》2008,320(5881):1309-1312
Binary pulsar systems are superb probes of stellar and binary evolution and the physics of extreme environments. In a survey with the Arecibo telescope, we have found PSR J1903+0327, a radio pulsar with a rotational period of 2.15 milliseconds in a highly eccentric (e = 0.44) 95-day orbit around a solar mass (M(middle dot in circle)) companion. Infrared observations identify a possible main-sequence companion star. Conventional binary stellar evolution models predict neither large orbital eccentricities nor main-sequence companions around millisecond pulsars. Alternative formation scenarios involve recycling a neutron star in a globular cluster, then ejecting it into the Galactic disk, or membership in a hierarchical triple system. A relativistic analysis of timing observations of the pulsar finds its mass to be 1.74 +/- 0.04 M solar symbol, an unusually high value. 相似文献
76.
Takaaki Ishii Atsushi Matsumura Sachie Horii Hino Motosugi Andre Freire Cruz 《Biology and Fertility of Soils》2007,44(1):217-222
A greenhouse experiment was conducted to examine the favorable effects of sod culture system with bahiagrass (Paspalum notatum Flügge.) and Vulpia myuros (L.) C. C. Gmel. intercropped with citrus trees on the establishment of the network of arbuscular mycorrhizal (AM) fungus
hyphae in their rhizospheres. Special acrylic root boxes with three compartments were used for the experiment. Four types
of citrus rootstock seedlings, trifoliate orange (Poncirus trifoliata Raf.), sour orange (Citrus aurantium L.), rough lemon (Citrus jambhiri Lush.), and Citrus natsudaidai Hayata, were separately transplanted into one outer compartment in each box, and the seedlings of bahiagrass and V.myuros were separately transplanted into the other outer compartment. An AM fungus, Gigaspora margarita Becker and Hall, was inoculated in the center compartment of each box. Some boxes with both outer compartments without plants
and with some plants in only one outer compartment were also prepared. The box with bare × bare had very low density of AM
hyphae. There were a few hyphae in bare compartments in the boxes of trifoliate orange × bare, sour orange × bare, rough lemon
× bare, and C. natsudaidai × bare. The density of hyphae in the compartments with citrus seedlings and grasses, however, was significantly higher than
in every bare compartment, and the hyphae in the compartments with plants penetrated deeply into the sand. In particular,
the density in the compartments of citrus seedlings increased when bahiagrass or V. myuros was transplanted as a neighboring plant. The percentage of AM fungus colonization in every plant root was high. New spore
formation was observed in compartments with plants, whereas there were few spores in every bare compartment. In particular,
the spore formation in bahiagrass compartments was superior to that in other compartments with plants. Our results suggest
that the network system by AM hyphae is easily discernible in the rhizospheres between citrus rootstocks and bahiagrass or
V. myuros, but bare ground severely inhibits the formation and development of AM hyphal network and reduces the number of AM spores
in the soil. 相似文献
77.
Ransom SM Hessels JW Stairs IH Freire PC Camilo F Kaspi VM Kaplan DL 《Science (New York, N.Y.)》2005,307(5711):892-896
We have identified 21 millisecond pulsars (MSPs) in globular cluster Terzan 5 by using the Green Bank Telescope, bringing the total of known MSPs in Terzan 5 to 24. These discoveries confirm fundamental predictions of globular cluster and binary system evolution. Thirteen of the new MSPs are in binaries, of which two show eclipses and two have highly eccentric orbits. The relativistic periastron advance for the two eccentric systems indicates that at least one of these pulsars has a mass 1.68 times greater than the mass of the Sun at 95% confidence. Such large neutron star masses constrain the equation of state of matter at or beyond the nuclear equilibrium density. 相似文献
78.
Four groups of six 21 days-old piglets were used to evaluate the effect of a prebiotic or probiotic on the intestinal fermentative activity. In each group, piglets received one of the following diets: basal diet (C); basal diet supplemented with xylo-oligosaccharide (C-XOS); basal diet supplemented with Saccharomyces cerevisiae (C-SC); and basal diet supplemented with xylo-oligosaccharide and S. cerevisiae (C-XOS + SC).
The short chain fatty acids in the colon of piglets were decreased with the inclusion of S. cerevisiae in the diet (P < 0.01). The xylanolytic activity was higher (P < 0.05) in the small intestine of piglets fed C-XOS + SC diet, but no significant differences were found in the caecum and colon. In the caecum contents, the cellulolytic activity was increased (P < 0.05) by the C-XOS and C-SC diets, but remained similar when the diet was supplemented with the two additives combined. 相似文献
79.
Dubey JP Navarro IT Graham DH Dahl E Freire RL Prudencio LB Sreekumar C Vianna MC Lehmann T 《Veterinary parasitology》2003,117(3):229-234
The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T. gondii oocysts in the environment because chickens feed from the ground. In the present study, prevalence of T. gondii in 40 free range chickens (Gallus domesticus) from a rural area surrounding Paraná, Brazil was assessed. Blood, heart, and brain from each chicken were examined for T. gondii infection. Antibodies to T. gondii, assayed with the modified agglutination test (MAT> or =1:5) were found in 16 chickens. Hearts and brains of seropositive (MAT> or =1:5) chickens were bioassayed in mice. Additionally, hearts and brains of seronegative (MAT<1:5) chickens were bioassayed in two T. gondii-free cats (12 chickens per cat). T. gondii was isolated from 13 of 16 (81%) seropositive chickens. Of the two cats fed tissues pooled form seronegative chickens, one shed T. gondii oocysts. Nine of the 13 T. gondii isolates killed 100% of infected mice. The T. gondii isolate from the cat was also virulent for mice. Genotyping of 13 chicken isolates of T. gondii using the SAG2 locus indicated that seven isolates were type I and six were type III; three of these type III isolates killed all infected mice suggesting that all strains virulent for mice are not type I. The isolate from the feces of the cat fed chicken tissues was type I. 相似文献
80.
The use of commercial catch statistics to estimate overfishing consequences has been criticised, but alternative long‐term data sets are rare. Long time‐series data sets from recreational fisheries competitions have been used to infer trends in coastal fish communities. Here an historic archive (1953–2007) of recreational spear fisheries in Galicia (NW Spain) was employed to estimate long‐term changes in coastal ecosystems. Using generalised additive regression models, decreases in the abundances of coastal rocky reef fishes of up to 76% over the last 50 years were found. In the same period, the mean body weight also decreased by 76%. In addition, relative catch frequency has decreased for the most valuable commercial species. Overfishing, amongst other human impacts, has brought these ecosystems so close to collapse that it is urgent to implement measures to ensure their recovery. 相似文献