Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen

Background

Since 1999, field evidence of transplacental infection by porcine circovirus type 2 (PCV2) and reproductive failure has been reported in pigs. The objective of this study was to evaluate the clinical and pathological consequences of PCV2 infection in conventional PCV2-seropositive gilts by insemination with PCV2b-spiked semen.

Results

Six PCV2 seropositive gilts were inseminated with PCV2b-supplemented semen (infected) and three animals with semen and cell culture medium (controls). Only three out of the six infected animals were pregnant by ultrasonography on day 29 after insemination, while two out of the three controls were pregnant. One control gilt aborted on day 23 after insemination but not due to PVC2. Viraemia was demonstrated in four out of six infected and in one control gilt that became infected with PCV2a. Anti-PCV2 antibody titres showed dynamic variations in the infected group throughout the study. Among infected gilts, the animal with the lowest anti-PCV2 titre (1/100) at the beginning of the experiment and another that reached a similar low value during the experiment showed evident seroconversion over time and had also PCV2 positive foetuses. One placenta displayed mild focal necrosis of the chorionic epithelium positively stained by immunohistochemistry for PCV2 antigen.

Conclusions

PCV2-seropositive gilts can be infected with PCV2 after intrauterine exposure and low maternal antibody titre may increase the probability of a foetal infection.     

气味指纹分析技术在食品质控和风味研究的应用

着重以食用油、橙汁和奶制品为例,阐述了Sensory Array Fingerprint Analyzer Method(气味指纹分析法)对食品所建立的新型质量控制和风味研究方法.经针对7种储存时间不同的食用油、脂(大豆油、菜籽油、猪油、牛脂、禽类脂、白色油脂、棕榈油)的过氧化值和酸价的分析模型的建立,展示了应用气味指纹分析技术(电子鼻)对食用油品质控制及抗氧化能力比较的新方法,对不同品质的橙汁(半年和一年半的鲜榨汁,和浓缩还原汁)、不同种类的鲜奶(灭菌全脂奶、灭菌半脱脂奶、半脱脂生奶和乳粉还原奶)和真伪某品牌奶粉的区分,表明该技术对复杂介质样品的品质变化具有独特的识别能力.分析结果表明,该分析技术具有以下优点满意的分析灵敏度和重复性传感器检测平均重复性为1.32%RSD(n=10),对同一样品测量的重复性为1.4%RSD(n=10),对所有油样的过氧化值和酸价测定具有良好的线性关系,平均相关系数为0.998 4.不同品质样品之间区分指数DI的特异性明显(＞90).所有的数据通过化学计量学方法处理,并且该分析方法无需样品前处理.     

Sprinkling-induced foliar injury to pepper plants: Effects of irrigation frequency,duration and water composition

Summary This study was conducted to determine the conditions and causes of foliar salt absorption and injury from sprinkler irrigation with saline water. Bell pepper plants (Capsicum annuum L. cv. Yolo Wonder B) grown in covered nutrient solution cultures in the greenhouse were sprinkled daily with NaCl and CaCl₂ waters for up to 10 weeks. Unsprinkled plants grown in nonsaline, and in one experiment, saline cultures were compared with plants sprinkled with waters containing different concentrations of NaCl and/or CaCl₂. Both the frequency and duration of sprinkling (up to 32 min each day) were tested.The results showed that Ca²⁺, Na⁺, and Cl^– were readily absorbed through the leaves at rates that were essentially linear functions of salt concentration and duration of sprinkling. Increasing frequency of sprinkling increased salt uptake and injury more than increasing duration. Sprinkling with either NaCl or CaCl₂ waters was more toxic to pepper than mixtures of the two salts. Although CaCl₂ was more toxic than NaCl, low concentrations of Ca²⁺ ameliorated the detrimental effects of NaCl waters. Foliar analyses indicated that leaf injury was not correlated with Cl^– accumulation. It appeared that it was caused directly by excessive cation accumulation or indirectly by the resultant ionic imbalance.Received for publicationSupervisory Plant Physiologist, Chemist, and Research Agronomist     

Yield and foliar injury responses of mature plum trees to salinity

Summary The salt tolerance of mature Santa Rosa plum trees was assessed on 20-year-old trees grown in the San Joaquin Valley of California. The experimental design consisted of six levels of irrigation water salinity (electrical conductivities of 0.3 to 8 dS/m) replicated five times with each replication consisting of ten trees. Salinity treatments imposed in March 1984 did not influence tree yields harvested in June 1984. In 1985, the second year of treatments, yield from the highest salt treatment (electrical conductivity of irrigation water, EC _i , of 8 dS/m) was reduced by half; the number of fruit harvested was reduced 40%, and fruit size was reduced significantly. Foliar damage was so severe by the end of 1985 that nonsaline water was applied to the two highest salt treatments (EC _i = 6 and 8 dS/m) in an attempt to restore tree vigor. In 1986 salt effects had become progressively worse on the continuing saline treatments. A linear piece-wise salt tolerance curve is presented for soil salinity values, expressed as the electrical conductivity of saturated extracts (EC _e ) integrated to a soil depth of 1.2 m over a 2-year period. The salt tolerance threshold for relative yield (Y _r ) based on 3 years of data was 2.6 dS/m and yield reduction at salinity levels beyond the threshold was 31% per dS/m (Y _ir=100 – 31 [EC _e – 2.6]j). Significant foliar damage occurred when leaf chloride concentrations surpassed 200 mmol/kg of leaf dry weight (0.7%). Sodium concentrations in the leaves remained below 10 mmol/kg (0.02%) until foliar damage became severe. This suggests that chloride was the dominant ion causing foliar damage.     

Salt tolerance of irrigated guayule

Summary The salt tolerance of guayule (Parthenium argentatum Gray cv. N565-II) was tested in small held plots (silty clay soil) in the Imperial Valley of California. Seedlings were transplanted in October 1981. Differential salination was begun in March 1982 and continued for 4 years by irrigating with waters salinized with NaCl and CaCl₂ (1:1 by wt.) to obtain electrical conductivities of 0.8, 1.4, 3, 6, 9, and 12 dS/m. Dry matter, rubber, and resin yields were determined from pollarded plants in February 1984 and uprooted plants in February 1985 and 1986. Rubber concentrations in the woody branches in 1984 and 1985 averaged 6.1 and 7.3%, respectively on a dry weight basis and were not significantly affected by soil salinity. Resin concentrations averaged 8.6% and 7.3% for the two years. In 1986, both rubber and resin concentrations decreased with increased salinity. Rubber and resin concentrations in the root crowns were approximately one percentage point less than those of the shoot. Dry matter and resin yields were not affected by salinity until the time- and depth-averaged electrical conductivity of the saturated-soil extracts ( ) taken from the rootzone (0–90 cm) exceeded 8.7 dS/m. Above 8.7 dS/m, both yields decreased 11.6% per dS/m increase in . Rubber yields decreased 10.8% per dS/m above a threshold of 7.8 dS/m. Plant mortality rather than growth reduction at high levels of salinity appears to be the limiting factor for rubber production from irrigated guayule.     

Effects of nucleo-cytoplasmic interactions on leaf volatile compounds from citrus somatic diploid hybrids

Three diploid citrus somatic hybrids (cybrids) were produced by fusions combining nucellar callus-derived protoplasts of Willow Leaf mandarin (Citrus deliciosa Ten.) and Commune clementine (Citrus clementina Hort. ex Tan.) with, respectively, leaf protoplasts of Eureka lemon [Citrus limon (L.) Burm.] and Marumi kumquat [Fortunella japonica (Thunb.) Swing.] and leaf protoplasts of Marumi kumquat. Ploidy and origins of the nuclear, chloroplastic, and mitochondrial genomes were investigated by flow cytometry and nuclear and cytoplasmic simple sequence repeat analyses. Volatile compounds were extracted from the leaves of the three cybrids by a pentane/ether (1:1) mixture, analyzed by GC-MS, and compared to those of their parents. The cybrids were found to be very close to their nucleus-giving parent, suggesting that the main information for volatile compounds biosynthesis is contained in the nucleus. However, nucleo-cytoplasmic interactions occurred: the (mandarin + lemon) cybrid, possessing nucleus and chloroplasts of lemon and mitochondria from mandarin, synthesizes more monoterpene alcohols and esters than its nucleus-giving parent; the (clementine + kumquat) cybrid, possessing nucleus from kumquat and organelles from mandarin, synthesizes more monoterpene and sesquiterpene hydrocarbons and sesquiterpene alcohols than its nucleus-giving parent.     

番茄形态结构模型参数的多目标拟合估算方法研究

对番茄生长模型参数进行准确估算是模型得以验证和面向应用的前提条件。基于番茄拓扑结构，采用非线性最小二乘优化方法，通过对植株形态数据的多目标拟合，实现模型参数的最优估计和校准。非线性最小二乘法的权值根据目标数据自动调整，使得不同量纲和不同数值范围的观测数据可以在同一水平上进行，提高了计算的精度和稳定性；另一方面用差商近似代替导数加快了算法的收敛速度。通过应用拟合优度检验，得出模型输出对观测值的拟合程度较好，说明模型合理，参数可靠。实践表明该估算方法是对模型进行验证的一个行之有效的方法。     

Long range transport and deposition of mineral matter as a source for base cations

Mineral dust in the atmosphere is generally alkaline, and is a source of base cations in precipitation. Annual emissions of particles from large combustion plants and industrial processes in Europe is of the order of 24 million tonnes, and the calcium content may be as high as 1.4 million tonnes. Emissions from diffuse sources such as agricultural activities, construction and quarrying are much less well known. Emissions of dust from the Sahara have been estimated to more than 200 tonnes, with a calcium content of 3–5%. In northern Europe, airborne concentrations of calcium and the concentration of calcium in precipitation are generally consistent with the anthropogenic emissions and their regional distribution. Transport of dust from Sahara is a major source of base cations in precipitation around the Mediterranean Sea, but the influence diminishes further north. The concentration of calcium in precipitation decreases from south to north and from east to west in Europe. Dry deposition of alkaline particles is not well documented, but may be at least as high as the input by precipitation in regions where there are large emissions. There are still large uncertainty gaps with respect to emissions, transport and deposition of calcium-containing particles.