Rescue of dystrophic muscle through U7 snRNA-mediated exon skipping

Most mutations in the dystrophin gene create a frameshift or a stop in the mRNA and are associated with severe Duchenne muscular dystrophy. Exon skipping that naturally occurs at low frequency sometimes eliminates the mutation and leads to the production of a rescued protein. We have achieved persistent exon skipping that removes the mutated exon on the dystrophin messenger mRNA of the mdx mouse, by a single administration of an AAV vector expressing antisense sequences linked to a modified U7 small nuclear RNA. We report the sustained production of functional dystrophin at physiological levels in entire groups of muscles and the correction of the muscular dystrophy.     

Characterization of a calcium-soluble protein fraction from yellow mustard (Sinapis alba) seed meal with potential application as an additive to calcium-rich drinks

A calcium-soluble protein isolate (CSPI) was prepared from the supernatant obtained after addition of 0.75 M calcium chloride to a pH 5.0 aqueous extract of yellow mustard (Sinapis alba) seed meal. Total amino acid analysis showed that the CSPI has significantly higher (p < 0.05) contents of glutamic acid + glutamine, cysteine, and proline when compared to the precipitated, calcium-insoluble proteins. Peptide mass fingerprinting of tryptic peptides of the major polypeptides by mass spectrometry indicated that the CSPI is composed mainly of cruciferin proteins with a contribution from napins (the major allergenic proteins of S. alba). The S. alba CSPI had significantly higher (p < 0.05) protein solubility and emulsion formation ability in the presence of 0.75 M calcium chloride when compared to similar isolates prepared from Brassica juncea (brown mustard) and soybean seed meals. We suggest that the S. alba CSPI could be used to prepare calcium-fortified high protein liquid products. However, the presence of allergenic proteins in this extract may limit its widespread food use.     

NMR relaxation and water self-diffusion studies in whey protein solutions and gels

The changes in water proton transverse relaxation behavior induced by aggregation of whey proteins are explained in terms of the simple molecular processes of diffusion and chemical exchange. The water self-diffusion coefficient was measured in whey protein solutions and gels by the pulsed field gradient NMR method. As expected, water self-diffusion was reduced with increased protein concentrations. Whatever the concentration, the water molecules were free to diffuse over distances varying from 15 to 47 mum. Water diffusion was constant over these distances, demonstrating that no restrictions were found to explain the water hindrance. The modification in protein structure by gelation induced a decrease in water diffusion. The effects of protein concentration on water diffusion are discussed and modeled. Two approaches were compared, the obstruction effect induced by a spherical particle and the cell model, which considered two water compartments with specific self-diffusion coefficients.     

Spaced plant evaluation of Mediterranean germplasm collections of perennial ryegrass

Summary A sample of 50 wild populations of perennial ryegrass (12 from northern Italy and 38 from Corsica) was tested with controls as spaced plant nurseries in two locations in France. Multivariate analyses, such as principal component analysis, hierarchical clustering and factorial discriminant analysis were used to describe the genetic variability of such Mediterranean material.On the whole, Mediterranean populations do not appear to be more promosing than the north European varieties. However, some populations from Corsica could be used to develop genetic resources for the Mediterranean area.The interest of each cluster is discussed and geographical repartition of clusters is shown on country maps.The factorial discriminant analysis gives a linear discriminant Fisher function which clearely discriminates two groups of populations according to their country of origin.     

Cocoa-based agroforestry system dynamics and trends in the Akongo subregion of central Cameroon

Cocoa is a major crop and income source for most farmers and rural households in the Centre, South and South-West regions of Cameroon, where cocoa is generally produced in agroforestry systems. In this country, cocoa-based agroforestry systems (CBAFS) are undergoing multiple changes alongside the rapid changes underway in the natural, economic and socio-political conditions. This study—carried out in the Akongo subregion in central Cameroon—was designed to gain insight into the CBAFS trends and dynamics in the light of those multiple changes. This semi-structured socioeconomic survey involved interviews and direct observations at plot, farm, household and village scales. Overall, forty cocoa growers from ten villages were interviewed and then fifteen cocoa plots were characterized on the basis of the survey findings. They revealed that cocoa was the major crop in this study area, with cocoa plantations occupying three quarters of the total farming area. Three types of CBAFS were identified, which differed according to their vegetation structure, management practices and age of the plantations. Dynamics affecting the structural characteristics and the spatial extension of these systems emerged and were intimately linked to the dynamics of the cocoa farmer population in relation to their context.

     

Diversity in the chemical composition and digestibility of leaves from fifty woody species in temperate areas

Agroforestry Systems - In temperate regions, tree leaves could be a source of energy, protein, vitamins and minerals for ruminants. Our objective was to study the diversity in chemical composition...     

Myosin,parvalbumin and myofibril expression in barbel (Barbus barbus L.) lateral white muscle during development

Histo- and immunohistochemical techniques have recently been used to study the fibre type and myosin expression in fish muscle during development. In the present work, embryonic, larval and adult myosin isozymes (heavy and light chains) and parvalbumin isotypes were analyzed, from fertization to the adult stage, by polyacrylamide gel electrophoresis of barbel (Barbus barbus L.) trunk muscle extracts. The examined myosins display the sequential transitions from embryonic to larval and adult forms characteristic of higher vertebrates. They are characterized by specific heavy chains but their light chains differ only by the LC₁/LC₃ stoichiometry with LC₃ exceeding LC₁ after 10 days. Sarcoplasmic parvalbumins show considerable and unforeseen developmental transitions in their isotype distribution: the PA II isotype first appears after hatching and becomes the predominant form until the length reaches about 6 cm. One month after hatching, the amount of PA II then decreases and the synthesis of PA III and IV further increases to reach the typical adult pattern at a size of 18 cm. These observations show that the distribution of parvalbumin isotypes reflects the stage of development. It suggests a specific role for each isotype in relation to muscle activity. Microscopy illustrates the progressive development of somites, muscles cells, and myofibrils, which accelerates at hatching when movements increase.