Outlier identification of soil phosphorus and its implication for spatial structure modeling

Outliers are classified as global outliers and spatial outliers. Up to now, there is little information about the outliers especially the spatial outliers and their influence on the spatial structure modeling of soil properties. A total of 537 soil samples were collected based on a 30 × 30 m grid in a permanent dairy farm in southeast Ireland. Graphic methods of histogram and box plot combined with Moran’s I were applied to detect the outliers of soil phosphorus (P). Sixteen outliers (5 global outliers and 11 spatial outliers) of soil P were found in the study area. Compared to the raw data, the data with global outliers excluded always had the larger global Moran’s I value indicating a stronger spatial autocorrelation. Clear spatial clusters (High–High and Low–Low clusters) were observed based on local Moran’s I. The High–High spatial clusters were located around the main farm yard and near the traffic route due to more intensive management by farmers. The Low–Low spatial clusters were mainly close to the river. For these areas, P fertilizer or slurry should be applied for healthy grass growth. The dataset with outliers excluded had a reliable semi-variogram model with a low nugget/sill ratio (32.4 %), which was closed to its corresponding transformed data (30.5 %). The cross-validation results revealed that the dataset without outliers had the strongest linear regression model (r = 0.768), indicating that the outliers played an important role in the spatial structure modeling.     

Specific inhibition of plaque formation to phosphorylcholine by antibody against antibody

Spleen cells from BALB/c mice immunized with heat-killed rough pneumococci (strain R36A) or spleen cells from normal mice immunized in vitro with the same antigen produce direct hemolytic plaques against sheep erythrocytes coated with pneumococcal C polysaccharide or conjugated with phosphorylcholine. Formation of plaques is specifically inhibited by phosphorylcholine or by antiserum to mouse immunoglobulin A myeloma protein which binds phosphorylcholine. Thus, the myeloma proteins and normal BALB/c antibodies share similar idiotypic determinants. This experimental system is suitable for probing the role of the antigen receptor in the immune response.     

藏北黑颈鹤繁殖前期的昼间活动时间分配

2006年4～6月，采用焦点动物取样法和所有事件记录法，对藏北黑颈鹤Gins nigicollis繁殖前期的昼间行为进行了详细观测。结果表明：①黑颈鹤昼间行为活动时间分配具有一定的节律性。各种行为所占时间百分比大小依次为：取食43．69％，警戒21．78％，保养21．69％，运动7．57％，杂项3．18％，繁殖2．09％。②各种行为活动的时间分配在雌雄个体间具有一定的差异。雄鹤的警戒和运动行为比雌鹤高，且警戒行为具有极显著差异（P〈0．01），其他4种行为雌性较高。③黑颈鹤的各种行为表现出一定的节律性。11：00以前，各种行为活动相对稳定，11：00之后依次出现了3个取食高峰和1个保养高峰，且呈上升趋势；其他4种行为无明显峰值，并呈下降趋势。④雌雄鹤的取食、警戒和保养行为在不同时段上表现出同步性和异步性。雄鹤取食高峰集中在中午（12：00～14：00），雌鹤的取食高峰分散在一天中的早（8：00），中（12：00，14：00）和晚（17：00）3个时期。讨论了繁殖前期黑颈鹤性别间的行为差异，并与黑颈鹤东部种群以及其他鹤类的行为进行了对比。图2表1参35     

Effects of permanent magnetic fields on the proliferation of Phalaenopsis protocorm-like bodies using liquid medium

Magnetic fields (MFs) have been applied for the first time in orchid micropropagation. Protocorm-like bodies (PLBs) – approximately 3 mm in diameter – first derived from leaf segment culture of Phalaenopsis Gallant Beau ‘George Vazquez’, were subcultured every 2 months, and served as initial explants. The proliferation of Phalaenopsis PLBs in liquid medium in the Miracle Pack^® culture system was affected by the action of different intensities and polarities of MFs: 0.1, 0.15 and 0.2 Tesla (T) at North (N) and South (S) poles. The MF of 0.1 T – S resulted in the greatest fresh weight of regenerated PLBs. The average number of neo-PLBs formed per clusters in the PLB treated by MF: 0.1–0.2 T was decreased compared to the control exposed to natural MF (5 × 10⁻⁶ T). The proliferation of PLBs under 0.15 T – MF at both N and S poles for 2 and 7 weeks demonstrated that a longer duration of exposure to an MF of 0.15 T, regardless of the polarity, resulted in greater biomass of newly formed PLBs and smaller average number of newly formed PLBs. The S pole of MF had stronger effects on Phalaenopsis PLBs proliferation than the N pole did in all treatments.     

Spatio-temporal impacts of roads on the persistence of populations: analytic and numerical approaches

Roads can have drastic impacts on wildlife populations. Although there is wide recognition of the negative impacts caused by roads and a wealth of practical studies, there is a lack of theoretical work that can be used to predict the impact of road networks or to implement mitigation measures. Here, using Skellam’s diffusion model, we develop analytic and numerical approaches to analyze the impact of road networks on the survival of populations. Our models show that the viability of a population is determined not only by road density but also by the size and shape of patches. Accordingly, we studied the minimum size of a patch to sustain a population with given diffusion and growth parameters. We provide simple formulas to estimate the minimum patch size, and illustrate the importance of shape with square and rectangular patches. Our models also allow the estimation of time to extinction after road construction for a population in a patch smaller than that of the minimum size. Finally, using numerical computations we illustrate how the spatial arrangement of fences strongly affects both the equilibrium density and the spatial distribution of populations, and that not all fence layouts are equally effective. We anticipate that our methods provide a tool to assess the impact of geometrical features of road networks on wildlife and that they can be used to design mitigation measures to prevent the decline and extinction of populations in an anthropogenically disturbed landscape.     

Comparative influence of isolation,landscape, and wetland characteristics on egg-mass abundance of two pool-breeding amphibian species

The distribution and abundance of species are shaped by local and landscape processes, but the dominant processes may differ with scale and increasing human disturbance. We investigated population responses of two pool-breeding amphibian species to differences in local and landscape characteristics in suburbanizing, southeastern New Hampshire, USA. In 2003 and 2004, we sampled 49 vernal pools for spotted salamander (Ambystoma maculatum) and wood frog (Lithobates sylvaticus) egg masses. Using egg masses as a proxy for breeding-female population size, we examined the relative influence of five land-use and three isolation variables at two scales (300 and 1000 m) and five wetland variables on egg-mass abundance. For both species, road density at the landscape scale (1000 m) and hydroperiod most strongly predicted egg-mass abundance, with abundance decreasing as roads became denser and hydroperiods shortened. Wetland isolation was also an important predictor, with abundance greatest at more isolated pools, suggesting that both species concentrate at isolated pools when alternative breeding sites are scarce. Surprisingly, no 300-m parameters were strongly associated with salamander egg-mass abundance, whereas several landscape parameters were. In suburbanizing areas, it is at least as important to consider landscape-scale road density as to consider hydroperiod when designing conservation plans for these species. Furthermore, both isolated and clustered pools provide these species important habitat and may require protection. Finally, the conceptual framework for spotted-salamander management must be expanded so that spatial configuration at the landscape scale becomes a regular, integrated component of conservation planning for this species.     

Graph-theoretic connectivity measures: what do they tell us about connectivity?

Graph-theoretic connectivity analyses have received much attention in connectivity evaluation during the last few years. Here, we explore the underlying conceptual differences of various graph-theoretic connectivity measures. Based on connectivity analyses from three reserve networks in forested landscapes in Central Finland, we illustrate how these conceptual differences cause inconsistent connectivity evaluations at both the landscape and patch level. Our results also illustrate how the characteristics of the networks (patch density) may affect the performance of the different measures. Many of the connectivity measures react to changes in habitat connectivity in an ecologically undesirable manner. Patch prioritisations based on a node removal analysis were sensitive to the connectivity measure they were based on. The patch prioritisations derived from different measures showed a disparity in terms of how much weight they put on patch size versus patch location and how they value patch location. Although graphs operate at the interface of structure and function, there is still much to do for incorporating the inferred ecological process into graph structures and analyses. If graph analyses are going to be used for real-world management and conservation purposes, a more thorough understanding of the caveats and justifications of the graph-theoretic connectivity measures will be needed.     

In vitro digestibility of α-casozepine, a benzodiazepine-like peptide from bovine casein, and biological activity of its main proteolytic fragment

α-Casozepine is a peptide, corresponding to the sequence 91-100 of the bovine α(s1)-casein, displaying anxiolytic activity in the rat. The α(s1)-casein tryptic hydrolysate containing this peptide decreases stress effects after oral administration in various species including man. Therefore, the stability of this peptide toward gastric and pancreatic proteases has been assessed by using pepsin, chymotrypsin/trypsin, Corolase PP, pepsin followed by chymotrypsin/trypsin or pepsin followed by Corolase PP. α-Casozepine was slowly degraded by chymotrypsin, much more sensitive to pepsin and Corolase PP but not completely destroyed after 4 h kinetics. The bonds in the region 91 to 95 of the α-casozepine were totally resistant to hydrolysis by all studied proteases. Surprisingly, a fragment, corresponding to the sequence 91-97 and found in all the hydrolysis media in significant amount, possessed an anxiolytic activity in three behavioral tests measuring this parameter. This peptide could participate in the in vivo activity of α-casozepine.     

Evaluation of fertigation scheduling for sugarcane using a vadose zone flow and transport model

Micro-irrigation has become an optimal means for providing water and nutrients to crops. There is an ample space for improving fertilizer use efficiency with micro-irrigation, if the movement and reactions of fertilizers in the soil are well understood. However, the rhizosphere dynamics of nutrients is very complex, depending on many factors such as soil temperature, pH, water content, and soil and plant characteristics. Many factors cannot be easily accurately quantified. However, using state-of-the-art modelling techniques, useful and reliable information can be derived.An attempt was made to evaluate the reactive transport of urea in the root zone of a sugarcane crop under drip irrigation, and to quantify the fluxes of urea, ammonium, and nitrate into the crop roots, volatilization fluxes, and deep drainage using a numerical model. This quantification helped in designing an optimal fertigation schedule. Various parameters used in the model were taken from either the literature or the field study. A typical scenario, based on the recommended total quantity of urea for sugar cane crop under drip irrigation in India, was tested using HYDRUS-2D. The total amount of urea was divided into fortnightly doses, depending on the stage of crop growth. For this scenario, the modelled crop uptake was found to be 30% higher than the crop demand. Consequently, an optimal fertigation schedule was developed that reduced the use of urea by 30% while at the same time providing enough N for its assimilation at all stages of crop growth. This type of modelling study should be used before planning field experiments for designing optimal fertigation schedules.