Hypertrophic osteopathy associated with pulmonary Eikenella corrodens infection in a dog

CASE DESCRIPTION: A 4-year-old English Pointer was examined because of chronic, progressive signs of pulmonary disease and failure to respond to medical treatment. CLINICAL FINDINGS: At examination, radiography of the thorax revealed increased pulmonary opacity and air bronchograms in the right caudal lung lobe, and radiography of the forelimbs revealed periosteal bone production typical of hypertrophic osteopathy. Attempts to isolate a causative organism included bacterial culture of bronchoalveolar lavage fluid and a lung tissue specimen obtained via fine-needle aspiration. Despite a cytologic diagnosis of septic suppurative inflammation in the lavage fluid, those specimens did not yield bacterial growth. However, a biopsy specimen obtained during a lung lobectomy procedure yielded growth of Eikenella corrodens. TREATMENT AND OUTCOME: Despite successful surgical removal of the consolidated lung lobe and initiation of antimicrobial treatment, the dog died 6 days after surgery. CLINICAL RELEVANCE: The authors are not aware of reports of pulmonary infection with E. corrodens in animals. Infection with the organism is rare, but it is possible that infections are underreported given that the organism is difficult to culture and biopsy may be necessary to obtain enough tissue to yield a diagnosis.     

Serum IgG and total protein concentrations in dairy calves fed two colostrum replacement products

OBJECTIVE: To evaluate effects of 2 commercially available colostrum replacement products on serum IgG and total protein concentrations in dairy calves. DESIGN: Prospective clinical trial. ANIMALS: 84 Holstein bull calves from a single dairy. PROCEDURES: Calves were randomly assigned to be given 4 quarts of colostrum (group 1; n = 21), 2 packages of a colostrum replacement product (product A; group 2; 21), 1 package of a different colostrum replacement product (product B; group 3; 21), or 2 packages of product B (group 4; 21). Treatments were given within 3 hours after birth, and blood samples were collected 24 hours later and submitted for determination of serum total protein and IgG concentrations. RESULTS: Group 1 calves had significantly higher serum total protein and IgG concentrations than did calves in the other 3 groups. However, the percentage of calves with adequate passive transfer (ie, serum IgG concentration > 1,000 mg/dL) was not significantly different among groups 1 (90%), 3 (81%), and 4 (95%). In contrast, only 10% of calves in group 2 had adequate passive transfer. It was predicted that calves fed product B that had serum total protein concentrations > 5.2 g/dL would have serum IgG concentrations > 1,000 mg/dL at least 90% of the time. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that product B could be considered as an alternative to colostrum in dairy calves, but product A failed to routinely provide adequate serum IgG concentrations when fed according to label directions.     

Nocardia infections in cats: a retrospective multi-institutional study of 17 cases

OBJECTIVE: To record 17 cases of nocardiosis in cats from eastern Australia and to compare this series with cases previously reported. DESIGN: Retrospective/prospective study. RESULTS: Nocardia spp infections were diagnosed in 17 cats over 14 years from the three eastern states of Australia. There were no isolates from dogs during this period, but one isolate from a koala and two from dairy cows. The majority of cats presented with spreading lesions of the subcutis and skin associated with draining sinus tract(s). Early cutaneous lesions consisted of circumscribed abscesses. Infections spread at a variable rate, generally by extension to adjacent tissues. Lesions were generally located in regions subjected to cat bite or scratch injuries, including limbs, body wall, inguinal panniculus and nasal bridge. In some other cases, lesions were situated on distal extremities. The clinical course was variable, from chronic, indolent, initially localised infections to acute fulminating disease. Of the 17 cats, 14 were domestic crossbreds and three were purebreds. There was a preponderance of male cats (12 castrated, 1 entire young adult, 1 entire kitten). Nine of 17 cats were 10 years or older. Interestingly, the majority of infections were attributable to N nova. Immediate and/or predisposing causes could be identified in all cases, and included: renal transplantation [one cat]; chronic corticosteroid administration [three cats]; catabolic state following chylothorax surgery [one cat]; fight injuries [seven cats]; FIV infections [three of seven cats tested]. Of the 17 cats, three were apparently cured. Four were thought to be cured, but infection recurred after several months. Three cats responded partially but were euthanased, while another was improving when it died of unrelated complications. Two died despite treatment and two were euthanased without an attempt at therapy. For two cats there were either insufficient records or the patient was lost to follow up. CONCLUSION: Nocardiosis is a rare, serious disease. Currently it is more common in cats than dogs. Nocardial panniculitis may be clinically indistinguishable from the syndrome caused by rapidly growing mycobacteria. Although the prognosis is guarded, patients with localised infections caused by N nova often respond to appropriate therapy. If definitive treatment is delayed because of misdiagnosis, the disease tends to become chronic, extensive and refractory. Insufficient duration of therapy leads to disease recurrence.     

Staphylococcal skin disease in livestock

This review covers cutaneous manifestations of staphylococcal infection in livestock species. CATTLE: In cattle, staphylococcal infections may present as folliculitis or as impetigo. Both may present as mild forms of a group of conditions loosely termed udder dermatitis, which has various clinical presentations and does not always involve staphylococci. GOATS: In goats, staphylococcal infection may be secondary to chorioptic mange or contagious pustular dermatitis (parapox virus infection). While Staphylococcus aureus is usually implicated, infection with Staphylococcus chromogenes and Staphylococcus hyicus have also been reported. SHEEP: Ovine staphylococcal dermatitis typically involves the head. Trauma due to the close contact of heads over feeding troughs and abrasive plants at pasture may be predisposing factors. PIGS: In pigs, the most common cause of staphylococcal skin disease is S. hyicus, although other bacteria, including Staphylococcus sciuri, Staphylococcus chromogenes and meticillin-resistant S. aureus (MRSA), have also been isolated from some cases of greasy pig disease (exudative epidermitis). DIAGNOSIS: Routine culture methods are increasingly supplemented by molecular methods to characterize staphylococci. MANAGEMENT: As commensal bacteria, staphylococcal skin infection is presumed to develop because of predisposing factors. While topical and systemic therapies can be effective, it is important to control for predisposing factors so that recurrences can be prevented. LIVESTOCK-ASSOCIATED MRSA: In recent years, MRSA strains have emerged, particularly in pigs and cattle. While they rarely cause skin disease, they do pose a significant concern for public health authorities. Studies on livestock-associated MRSA may help to progress our understanding of staphylococci in livestock, especially how they spread between animals and humans.     

Isolation of Actinomyces hyovaginalis from sheep and comparison with isolates obtained from pigs

Actinomyces hyovaginalis, an organism initially described from pigs, was recovered from nine sheep and a moufflon. Further strains of A. hyovaginalis were recovered from five samples from pigs over the same period. 16S rRNA sequencing and extensive phenotyping demonstrated high similarity between the ovine and porcine isolates; however differences with respect to erythritol, adonitol and l-arabitol fermentation were detected. Ovine isolates were made from various sample sites including abscesses and highlight the importance of the accurate identification of the various coryneform isolates which affect sheep. A. hyovaginalis can be added to the growing list of coryneforms which can cause disease in sheep including Corynebacterium pseudotuberculosis, Trueperella pyogenes and Arcanobacterium pluranimalium.     

Studies on the Motility and Cryopreservation of Rainbow Trout (Salmo Gairdneri) Spermatozoa

The very short duration of vigorous movement (1 1/2 to 7 min) in fresh water and physiological solutions make trout spermatozoa difficult subjects for cryopreservation studies. Solutions consisting of 250 to 280 mmol sucrose and 5 to 12% dimethyl sulphoxide (DMSO) (4 parts) did not activate trout spermatozoa (1 part), but after dilution with fresh water vigorous motility could be fully restored. These sucrose-DMSO solutions were employed in cryopreservation studies. Using straws and a fast freezing — fast thawing procedure, post-thaw dilution with fresh water resulted in 25%-60% of spermatozoa becoming motile, all with vigorous forward progression. Some existing methods for the cryopreservation of other freshwater fish spermatozoa were repeated on trout without success.     

Implementing Sustainable Forest Management Using Six Concepts in an Adaptive Management Framework

Certification and principles, criteria and indicators (PCI) describe desired ends for sustainable forest management (SFM) but do not address potential means to achieve those ends. As a result, forest owners and managers participating in certification and employing PCI as tools to achieving SFM may be doing so inefficiently: achieving results by trial-and-error rather than by targeted management practices; dispersing resources away from priority objectives; and passively monitoring outcomes rather than actively establishing quantitative goals. In this literature review, we propose six concepts to guide SFM implementation. These concepts include: Best Management Practices (BMPs)/Reduced Impact Logging (RIL), biodiversity conservation, forest protection, multi-scale planning, participatory forestry, and sustained forest production. We place these concepts within an iterative decision-making framework of planning, implementation, and assessment, and provide brief definitions of and practices delimited by each concept. A case study describing SFM in the neo-tropics illustrates a potential application of our six concepts. Overall our paper offers an approach that will help forest owners and managers implement the ambiguous SFM concept.     

An Exploratory,Post-Harvest Comparison of Ecological and Economic Characteristics of Forest Stewardship Council Certified and Uncertified Northern Hardwood Stands∗

ABSTRACT

As more forest entities worldwide consider pursuing Forest Stewardship Council (FSC) certification, a critical question remains on whether stand-level management impacts differ between certified and uncertified forests. To begin to answer this question, we measured forest structure on three FSC-certified stands, three uncertified stands, and six adjacent unharvested reference stands (12 stands total) composed primarily of sugar maple (Acer saccharum) on non-industrial private properties in central Vermont, USA. The certified and uncertified partial harvests reduced total tree biomass and live tree carbon storage by one-third compared to reconstructed pre-harvest conditions. Both treatments also contained significantly lower densities of saplings and some mid-size trees compared to non-harvested references due to similar impacts from harvesting. The net present value of merchantable sugar maple over 10 year projections was consistently lower on certified than uncertified stands, but this difference was insignificant at discount rates from 4–8%. The certified stands contained significantly greater total residual volumes of coarse woody debris (standing and downed) than uncertified stands, although the debris was smaller than that found in unmanaged mature forests. Overall, our data suggest that FSC-certified harvested stands in northern hardwood forests have similar sugar maple timber value, aboveground live tree carbon storage value, similar live tree structure, and greater residual coarse woody debris than uncertified harvested stands.