全文获取类型
收费全文 | 643篇 |
免费 | 38篇 |
国内免费 | 1篇 |
专业分类
林业 | 13篇 |
农学 | 7篇 |
基础科学 | 1篇 |
66篇 | |
综合类 | 87篇 |
农作物 | 26篇 |
水产渔业 | 22篇 |
畜牧兽医 | 406篇 |
园艺 | 5篇 |
植物保护 | 49篇 |
出版年
2023年 | 5篇 |
2021年 | 5篇 |
2020年 | 6篇 |
2019年 | 11篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 19篇 |
2015年 | 8篇 |
2014年 | 13篇 |
2013年 | 25篇 |
2012年 | 27篇 |
2011年 | 40篇 |
2010年 | 12篇 |
2009年 | 18篇 |
2008年 | 22篇 |
2007年 | 30篇 |
2006年 | 26篇 |
2005年 | 22篇 |
2004年 | 13篇 |
2003年 | 27篇 |
2002年 | 24篇 |
2001年 | 28篇 |
2000年 | 22篇 |
1999年 | 25篇 |
1998年 | 4篇 |
1997年 | 8篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1993年 | 7篇 |
1992年 | 14篇 |
1991年 | 10篇 |
1990年 | 10篇 |
1989年 | 19篇 |
1988年 | 8篇 |
1987年 | 10篇 |
1986年 | 7篇 |
1983年 | 5篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1972年 | 7篇 |
1971年 | 5篇 |
1970年 | 7篇 |
1969年 | 7篇 |
1968年 | 10篇 |
1967年 | 4篇 |
1966年 | 3篇 |
1934年 | 4篇 |
排序方式: 共有682条查询结果,搜索用时 31 毫秒
101.
Taciana MF Silva Roberio G Olinda Carla MF Rodrigues Ant?nio CL Camara Francisco C Lopes Wesley AC Coelho Múcio FB Ribeiro Carlos IA Freitas Marta MG Teixeira Jael S Batista 《Veterinary research》2013,44(1):1
The present study was aimed at investigating the effect of experimental infection by Trypanosoma vivax in different stages of pregnancy, determining the pathogenesis of reproductive failure, and confirming transplacental transmission. We used 12 pregnant ewes distributed into four experimental groups: G1, was formed by three ewes infected with T. vivax in the first third of pregnancy (30 days); G2 comprised three infected ewes in the final third of pregnancy (100 days); G3 and G4 were composed of three non-infected ewes with the same gestational period, respectively. Each ewe of G1 and G2 was inoculated with 1.25 × 105 tripomastigotes. Clinical examination, determination of parasitemia, serum biochemistry (albumin, total protein, glucose, cholesterol, and urea), packed cell volume (PCV), serum progesterone, and pathological examination were performed. Placenta, amniotic fluid, blood and tissues from the fetuses and stillbirths were submitted to PCR. Two ewes of G1 (Ewe 1 and 3) presented severe infection and died in the 34th and 35th days post-infection (dpi), respectively; but both fetuses were recovered during necropsy. In G2, Ewe 5 aborted two fetuses on the 130th day (30 dpi) of pregnancy; and Ewe 6 aborted one fetus in the 140th day (40 dpi) of gestation. Ewes 2 and 4 delivered two weak lambs that died five days after birth. Factors possibly involved with the reproductive failure included high parasitemia, fever, low PCV, body score, serum glucose, total protein, cholesterol, and progesterone. Hepatitis, pericarditis, and encephalitis were observed in the aborted fetuses. The presence of T. vivax DNA in the placenta, amniotic fluid, blood, and tissues from the fetuses confirms the transplacental transmission of the parasite. Histological lesion in the fetuses and placenta also suggest the involvement of the parasite in the etiopathogenesis of reproductive failure in ewes. 相似文献
102.
GU-PCM2型控温式相变蓄冷冷藏车设计与空载性能试验 总被引:2,自引:2,他引:0
现有蓄冷冷藏车蓄冷装置多位于车厢顶部,存在重心偏高、不可控温等问题,基于此,该文设计了一款集相变蓄冷单元、车载制冷系统、隔热车厢、送风系统等于一体的GU-PCM2型蓄冷冷藏车。该蓄冷车将蓄冷装置独立设置于车厢前端并保温,系统利用夜间低谷电进行充冷,可在-25~10℃之间根据需要调控车厢温度。对蓄冷冷藏车厢设定温度为0和-18℃的2种工况进行空载温度场仿真与测试。结果表明,车厢内各测温点的模拟温度与实测温度均方根误差分别为0.7和0.8℃,最大绝对误差分别为1.1和1.2℃。冷藏车可有效控温10 h以上,车厢平均温度分别在1.1~2.9℃和-14.8~-16.9℃之间,波动范围为1.8和2.1℃,温度不均匀度系数在1.0以内。控温式蓄冷冷藏车与传统蓄冷车的对比试验结果表明,其平均温度波动值降低48.7%,温度绝对不均匀度系数降低50%以上,车厢质心较顶置式蓄冷车下降25.9%。研究结果可为蓄冷冷藏车的进一步优化设计与应用提供参考。 相似文献
103.
Mokwe-Ozonzeadi Ngozi Foster Ian Valsami-Jones Eugenia McEldowney Sharron 《Journal of Soils and Sediments》2019,19(2):946-963
Journal of Soils and Sediments - This study aims to identify a suitable sediment compartment for sediment quality monitoring by: (a) studying the concentration of trace metals (Cd, Cu, Ni, Pb and... 相似文献
104.
105.
Chen SJ Foster WR Jure-Kunkel MN Girit E Abraham R Hefta LJ Gao S Yonan CR Lin JH Dambach DM 《Veterinary immunology and immunopathology》2008,126(3-4):377-381
CD137 plays an important role as a co-stimulatory molecule in activated T cells. Agonistic CD137 specific antibodies have been investigated as therapeutic agents to promote tumor-specific immune responses by direct activation of T cells. As part of the pre-clinical pharmacological evaluation of cynomolgus monkeys, monkey CD137 was cloned and characterized. The deduced amino acid sequence encoded a full-length gene of 254 amino acids 95% identical to human CD137. Sequence variants identified in monkey CD137 include four splice variants lacking the transmembrane domain. These variants were detectable in human including two previously unreported variants. Two missense single nucleotide polymorphisms were detected present in 42 and 50% of 36 monkeys tested. In both monkey and human, mRNA expression of full-length CD137 and splice variants were significantly increased in peripheral blood mononuclear cells (PBMCs) upon stimulation by anti-CD3 antibodies. Recombinant monkey CD137 protein was bound with high affinity by an agonistic anti-human CD137 antibody but not by an anti-mouse CD137 antibody. In summary, compared to human, monkey CD137 showed distinct extracellular domain amino acid sequence and sequence polymorphisms. Thus, antibodies directed against epitopes in this extracellular domain could have differences in pharmacologic activity between cynomolgus monkeys and human or across individual cynomolgus monkeys. 相似文献
106.
107.
108.
109.
110.
D A Graham K A Mawhinney A German J C Foster B M Adair M Merza 《Journal of veterinary diagnostic investigation》1999,11(2):127-133
Isotype- and subclass-specific indirect enzyme-linked immunosorbent assays were developed to detect parainfluenza-3 virus-specific IgG1, IgG2, IgM, and IgA responses. Sera were treated with protein G-agarose prior to testing for specific IgM and IgA to eliminate the possibility of false-positive results due to IgM-rheumatoid factor and to remove interisotypic competition due to specific IgG. IgM and IgA absorbance values were expressed as a percentage of the absorbance values of positive reference sera included on each plate (S/P%), and respective positive/negative threshold values of 15.0% and 28.0% were determined. The mean interval between experimental infection of 3 calves and initial detection of specific IgG1 and IgG2 responses was 8.0 and 9.3 days respectively, rising rapidly to an initial plateau 13.7 and 11.0 days postinfection (dpi). Reinfection of these calves at 30 dpi resulted in further rapid increases, with higher plateau values reached 13.0 (IgG1) and 13.7 (IgG2) days later. The mean interval between infection and the first positive IgM and IgA responses was 6.7 and 12.3 days, respectively. IgM S/P% values peaked at 13.0 dpi, with all 3 calves showing a secondary anamnestic response to reinfection, peaking 4.7 days later. The IgA response to initial infection was weak, with only 2 calves showing an obvious peak response at 15.0 dpi. A strong anamnestic IgA response to reinfection occurred in 2 calves, with a peak response 9.5 days later. Apparent biphasic and triphasic IgM and IgA responses were evident in some calves. Acute and convalescent serum samples from 80 calves involved in 17 outbreaks of respiratory disease were tested for specific IgM and IgA. Positive IgM results were detected in 15 outbreaks, with 71 sera from 44 calves testing positive. Although IgA-positive results were detected in the same 15 outbreaks, only 42 sera from 31 calves were positive. In a previous study, seroconversion was detected in 21 of these calves from 10 outbreaks. Thus the diagnostic potential of the assays was in the order IgM > IgA > seroconversion. The correlations between IgM and IgA, IgM and seroconversion, and IgA and seroconversion results for each calf were 73.8%, 58.8% and 62.5%, respectively. 相似文献