Ecologically Based Strategies for Pest Control in Grassland Ecosystems

The researches were carried out in 2007, 2008 and 2009 at Cluj-Napoca （Romania）, in laboratory and field conditions （grassland ecosystems）, in four experiments. Our purpose was to study and use some ecological pest control methods based on indirect and direct strategies： （1） the harmful fauna monitoring in grassland ecosystems and useful fauna identification （by visual control, manually collecting, striking method on a tarpaulin）; （2） the use of some physico-mechanical and biotechnical methods （use of barriers-vessels, repellents, attractant traps-alimentary, visual） to control the pests. The researches concerning the indirect strategies, carried out from Mars 2007 to September 2009. There were identified 28 pest species and 29 useful ones. The direct strategies to control the pests consisted of 3 experiments. The use of colored traps with water （August 2008） on a meadow with ryegrass and clover, in 6 variants （white, silver, orange, green, blue and red） ＋ control （yellow）, recorded a very good efficacy at all variants excepting the red trap （variant 6）. The use of repellents （August 2008）, absinth decoction （against aphids and ants）, bracken extract （against aphids and snails）, common tansy decoction （against ants and soil pests） and kaolinite （against a large amount of pests）, recorded good results removing the pests. The attractant traps with alimentary baits （beer）, meant the use of 10 vessels on a 1000 m2 area, which captured 200 slugs （August 2009）. All three methods recorded a good efficacy against the pests.     

Identification and apoptotic potential of T-2 toxin metabolites in human cells

The mycotoxin T-2 toxin, produced by various Fusarium species, is a widespread contaminant of grain and grain products. Knowledge about its toxicity and metabolism in the human body is crucial for any risk assessment as T-2 toxin can be detected in processed and unprocessed food samples. Cell culture studies using cells of human origin represent a potent model system to study the metabolic fate of T-2 toxin as well as the cytotoxicity in vitro. In this study the metabolism of T-2 toxin was analyzed in a cell line derived from human colon carcinoma cells (HT-29) and primary human renal proximal tubule epithelial cells (RPTEC) using high-performance liquid chromatography coupled with Fourier transformation mass spectrometry (HPLC-FTMS). Both cell types metabolized T-2 toxin to a variety of compounds. Furthermore, cell cycle analysis in RPTEC proved the apoptotic effect of T-2 toxin and its metabolites HT-2 toxin and neosolaniol in micromolar concentrations.     

Water Pollution History of Switzerland Recorded by Sediments of the Large and Deep Perialpine Lakes Lucerne and Geneva

This review addresses the quantification of anthropogenic pollutants in lacustrine sediments by multidisciplinary analyses including: chronostratigraphy using radioisotopes (¹³⁷Cs) and radiocarbon dates (¹⁴C), trace metal analysis, faecal indicator analysis, as well as antibiotic-resistant genes by molecular analysis. Sediment cores from lakes Lucerne and Geneva that are located at a distance of 150?km from each other reveal a synchronous increase in anthropogenic trace metals (Pb, Cu, Zn, and Mn) following the industrial revolution in Europe about 1850. In both lakes, the peak of water pollution by toxic metals due to discharge of industrial wastewaters was reached in the middle of the twentieth century. During the second part of the twentieth century, both sites show a decrease in metal pollution following the implementation of wastewater treatment plants. On the contrary, the Vidy Bay of Lake Geneva where the treated wastewaters from the city of Lausanne are released since 1964 points out a dramatic increase in trace metal deposition. Later, a high increase in organic matter deposition, in bacteria (Escherichia coli and Enterococcus faecalis) activity as well as antibiotic-resistant genes and bacteria occurred into the bay, simultaneously with the eutrophication of the large and deep perialpine lakes in the 1970s due to excessive external nutrient loading.     

Impact of the potato inoculation date on potato virus Y load and viral distribution in daughter tubers at harvest

Aged plants are more difficult to infect than young plantlets. This modification of susceptibility is described as mature plant resistance (MPR). For potato virus Y (PVY), MPR is known to lead to low infection rates of plants inoculated at the postflowering stage and a decrease in the number of infected daughter tubers. However, the impact of inoculation date on the capacity of PVY to accumulate in daughter tubers has not been studied so far. Field and greenhouse experiments were carried out to better understand PVY epidemiology and to help potato growers to evaluate consequences of early/late infections on the quality of their crops. In field trials, potato plants (cv. Bintje) were covered by insectproof nets from planting to harvest except for a 14-day period to expose plants to natural PVY infections. Under controlled conditions, potato plants were mechanically inoculated with PVY at different dates from preflowering stages (early inoculations) to postflowering stage (late inoculations). At harvest, daughter tubers were individually collected and analysed to define proportions and viral load of infected tubers according to the time between virus inoculation and harvest. Our results showed that although the age of plants at the time of inoculation can modify their susceptibility to PVY infection, in return, early and late PVY inoculations lead to similar rates of infected tubers at the plant scale and equivalent viral accumulation in infected tubers. All together, these data revealed that both early/late infections are high risks for the sanitary quality of potato tubers.