Effect of human β-NGF gene-modified bone marrow-derived mesenchymal stem cells on rotational behavior of rats with Parkinson disease

AIM：To investigate the effect of human β-nerve growth factor (β-NGF) gene-modified bone marrow-derived mesenchymal stem cells (MSCs) transplantation on the rotational behavior improvement in a rat model of Parkinson disease (PD). METHODS：The rat model of PD was established successfully and the animals were divided into 4 groups:β-NGF-MSCs group (transplanted with 5×105 β-NGF-engineered MSCs), MSCs group (transplanted with 5×105 MSCs), DMEM/F12 group (5 μL transplantation medium was injected in the right striatum of the rats) and PD model group (without transplantation). The rotational scores were assessed 2 weeks, 4 weeks and 6 weeks after transplantation. At different time points after transplantation, the rats were tested for apomorphine (APO)-induced rotational behavior and the brains of the PD model rats were examined by fluorescence microscopy and immunohistochemical staining. RESULTS：Transplantation of human β-NGF gene-modified MSCs effectively improved the behavioral performance in the rats. At the 2nd, 4th and 6th weeks after cell transplantation, the rotational frequencies after injection of APO decreased significantly in β-NGF-MSCs group compared with MSCs group and PD group (P<0.05). Both β-NGF gene-modified MSCs and MSCs survived in the brains of PD model rats, had good compatibility with the host cells, and showed no signs of destroying the host and the glial cicatrisation. The β-NGF gene-modified MSCs expressed β-NGF stablely in the brains of PD model rats, and showed obvious improvement of the rotational behavior in the PD model rats induced by APO compared with MSCs group. CONCLUSION:The behavior of the rats with PD is significantly improved by transplanting β-NGF-modified MSCs in right striatum, and β-NGF gene therapy has potential clinical value．     

The upper limb vascular system modeling and simulation

In order to predict the physiological and pathological conditions of the humans, the study of hemodynamics information of arterial pulse wave is very important and valuable in cardiovascular. Regarding the blood vessels as a number of small flexible pipes in series, a electrical circuits model of the upper limb is presented according to Windkessel theory. Using Simulink and Simpowersystem of the matlab, the waveform of pressure can be simulated by the electrical circuits model. In view of hypertension and atherosclerosis is reflected by variations of dynamic parameters, we obtain waveform of the radial pressure and flow after simulation, and analyze the influence on pressure waveform in radial artery when the peripheral resistance and the compliance of the vessels changes respectively. These waveforms accord with the real physiological conditions, therefore the results show the effectiveness and validity of the model.     

近平滑假丝酵母(Candida parapsilosis)发酵普洱茶初探

以晒青茶为原料,接种专利菌株近平滑假丝酵母(Candida parapsilosis)GPT-5-11(专利号:201010182948.5)发酵普洱茶。结果表明,接种菌株的普洱茶样品GABA含量为0.3052~1.5332 mg/g,显著高于对照0.0628~0.1047 mg/g,其中灭菌每翻接种液态菌的样品GABA含量最高,为1.5332 mg/g,达到GABA茶的标准。同时,接种该菌株进行发酵后,发酵茶样中的主要功能成分含量均较自然发酵茶叶中的含量高,具有传统普洱茶的品质风味,且在香气和滋味方面有自身的特点,滋味醇和、厚滑,香气纯正略带乳香。     

利用酵母单杂交方法筛选大豆根融合基因表达cDNA文库

酵母单杂交体系通过筛选cDNA文库可直接得到与靶序列相互作用的蛋白质基因序列,是研究DNA-蛋白质相互作用和转录因子功能的最常用、最有效的方法之一.通过构建与Gal4p的激活结构域融合的表达cDNA文库,利用抗病相关基因启动子区的W-box顺式元件采用酵母单杂交方法对该文库进行了筛选,共得到4个阳性克隆.结果为进一步研究与顺式元件W-box相互作用的WRKY蛋白奠定了基础.     

基于无线传感器网络的农业温室环境测控系统研究

从无线传感器网络的体系结构、在温室环境中的应用等方面探讨了基于无线传感器网络的农业温室环境测控系统,为实现把无线传感器网络技术与温室环境相结合,达到智能化、自动化的目标,进而提高温室条件下农作物生产效益做出了一定的探索。     

中国农村家庭用能研究进展

农村能源问题一直是国内外学术界关注的焦点之一,国内关于农村家庭能源的研究主要集中于农村能源开发潜力评估、农村家庭用能消费模式、影响农村家庭用能消费的因素、农村家庭用能技术、农村家庭用能消费的生态环境效应、农村能源政策和农村可再生能源发展与建设问题等方面。为了给中国农村能源的深化研究与合理利用提供参考,综述了国内有关农村家庭能源的研究现状,分析了已有研究取得的成果以及存在的不足,并对其发展趋势进行了展望。     

蚕豆粉微波膨化的研究

在单因素试验的基础上,设计了微波功率、微波时间、糯米粉添加量及水分添加量的4因素L9(3)4正交试验,研究了微波对蚕豆粉膨化度及感官品质的影响.结果表明:利用微波膨化的蚕豆粉,整体呈灰白色,最高膨化率可达94%;入口酥脆,不顶牙,无腥味.正交试验最佳的工艺组合为:微波功率900 W,微波时间165s,糯米粉含量50%,水分添加量60%.水分添加量和糯米粉含量对蚕豆粉膨化度影响极显著,其中糯米粉可以改善蚕豆粉的膨化效果,而水分对膨化蚕豆粉的口感至关重要.     

不同施磷量对柱花草侧芽发生的影响

 为了探索不同磷肥施用量对柱花草侧芽发生的影响,以热研2号、有钩柱花草和电白柱花草为材料,设置了3个磷肥（P2O5）水平,分别为每千克土施入磷肥0.2,0.8,1.6g,并以不施磷肥为对照,在温室条件下进行盆栽试验。以累计主茎和侧枝的侧芽数量为指标进行了连续观测和分析。结果表明, 供试柱花草材料的主茎和侧枝的侧芽数是其遗传特性和栽培条件共同作用的结果。不同供试柱花草有利于其侧芽发生的最佳施磷量不同,热研2号柱花草最佳磷肥施用量为每千克土施入磷肥0.2g,其他2个供试柱花草的最佳磷肥施用量为每千克土施入磷肥0.8g。施用磷肥可以促进柱花草主茎侧芽和侧枝侧芽的发生,但对侧枝侧芽发生的影响更大,促进侧枝侧芽的发生可能是磷肥提高柱花草产量的重要途径。     

民族地区农家书屋建设现状分析——基于楚雄州姚安县前场镇9个农家书屋的调查

为充分掌握边疆民族地区农家书屋的建设及利用现状,对云南省楚雄彝族自治州姚安县前场镇的九个农家书屋在建设、管理和利用方面进行群众走访和问卷调查,进行问题分析和探讨,指出农家书屋存在的设备简陋、图书缺乏、管理人员素质不高及图书利用率低下等突出问题,并提出相应的对策和措施.     

基于GWR模型的耕地表层土壤有机质空间变异研究

通过选择耕地土壤有机质含量影响因素,设定量化标准、构建地理加权回归(GWR)模型,对2006-2010年忻州市忻府区耕地土壤有机质含量进行了统计及空间可视化分析,探索了不同影响因素对忻府区耕地土壤有机质含量的影响及其空间差异性.发现距村距离、海拔、地面坡度、田面坡度、通常地下水位、pH值、全N、地势起伏度、总户数等对忻府区耕地土壤有机质含量影响显著.