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排序方式: 共有323条查询结果,搜索用时 125 毫秒
101.
Monção Flávio Pinto Costa Marco Aurélio Morais Soares Rigueira João Paulo Sampaio de Sales Eleuza Clarete Junqueira Leal Dijair Barbosa da Silva Marcos Felipe Pereira Gomes Virgílio Mesquita Chamone Julieta Maria Alencar Alves Dorismar David da Cunha Siqueira Carvalho Cinara Murta José Eduardo Jardim Júnior Vicente Ribeiro Rocha 《Tropical animal health and production》2020,52(1):235-241
Tropical Animal Health and Production - The objective of this study was to evaluate the productivity, productive efficiency, and nutritional value of the elephant grass cultivar BRS capiaçu... 相似文献
102.
Radan Elvis Matias de Oliveira Giovanna Almeida Santoro Augusto Carlos da Bôaviagem Freire Fernanda Löffler Niemeyer Attademo Stella Almeida Lima Aline da Costa Bomfim Ana Bernadete Lima Fragoso Flávio José de Lima Silva Simone Almeida Gavilan Moacir Franco de Oliveira 《Anatomia, histologia, embryologia》2020,49(1):25-30
The aortic arrangement is an important structure associated with the maintenance of homeostasis. Based on this information, this study was conducted to describe the collateral arteries of the aortic arch of Antillean manatee and define the standard model for the species. Three specimens, an adult male, adult female and a male neonate, all strandings on the coast of the state of Rio Grande do Norte, Brazil, were used. The study was performed in the Laboratory of Morphophysiology of Vertebrates of the Federal University of Rio Grande do Norte, where in situ photographs were taken to demonstrate their topography. Subsequently, their hearts were removed and fixed in 10% formaldehyde and after 72 hr were dissected and analysed, obtaining schematic drawings and photographs of the vascular arrangement. The aortic arch was represented by three collateral arteries identified as the brachiocephalic trunk, left common carotid artery and left subclavian artery. This arrangement was similar to that found for other sirenians, and yet, for other mammals like hooded seal, murine, margarita island capuchin, black-handed tamarin, Mongolian gerbil and human. The morphological similarity presented in this study with different species of mammals, including humans, may contribute valuable information from an evolutionary point of view. 相似文献
103.
Brito Wildson Benedito Mendes Campos Milton César Costa de Souza Fernando Gomes Silva Laércio Santos da Cunha José Maurício de Lima Alan Ferreira Leite Martins Thalita Silva de Oliveira Flávio Pereira de Oliveira Ivanildo Amorim 《Precision Agriculture》2022,23(2):419-449
Precision Agriculture - Changes in primary cover for agricultural crops in Amazonas region influence the phenomenon of spatial variability in soil properties. This phenomenon is still studied... 相似文献
104.
H.?Bj?rn G.?EwaldEmail author P.?Sundin C.?Wesén J.?Skramstad P.?Fr?yen 《Fish physiology and biochemistry》2004,30(1):13-20
In order to study the metabolic fate of chlorinated fatty acids in fish, goldfish were fed either 9,10-dichlorostearic acid or oleic acid, chosen as the unchlorinated analogue, both radiolabelled at either the carboxyl (1st) or the terminal (18th) carbon of the fatty acid chain. By keeping the fish in hermetically closed aquaria, all the respired, assimilated and excreted radioactivity could be accounted for. Fish fed 9,10-dichlorostearic acid labelled in the terminal end respired radioactive CO2 to a much lower degree than fish fed the other test compounds. As a consequence, the radioactivity bound in lipids was higher in the group of fish fed dichlorostearic acid labelled in the terminal end. It is suggested that the chlorine atoms in the middle of the carbon chain obstruct the metabolic turn-over of 9,10-dichlorostearic acid, which may have an impact on the residence time of these compounds in the ecosystem. 相似文献
105.
Lenilton Santos Soares Ana Carolina Flôr Vieira Fernanda Fidler Leandro Guarezi Nandi Alcilene Rodrigues Monteiro 《Journal Of Aquatic Food Product Technology》2020,29(2):194-206
ABSTRACTIn the heat treatment of oysters, many aroma compounds are lost in the cooking steam and water. In this study, aroma compound profiles from oyster processing were identified by gas chromatography- mass spectrometry (GC-MS). The compounds were extracted by headspace-solid phase microextraction (HS-SPME), while the best process conditions such as temperature, extraction time, and desorption time were studied. The aroma compounds were obtained from raw oysters, from oysters after thermal treatment, from water released from raw oysters, from cooking steam, and from oyster boiling juice. All compounds were identified and semi-quantified by GC-MS. The major oyster aroma compounds were 1-octen-3-ol, hexanal, and benzaldehyde. Most of the components from oysters were lost both to the cooking steam and to the oyster boiling juice. The concentration range of volatile organic compounds transferred for oyster boiling juice between 14 and 90 mg L?1 shows that the residues from oyster processing have great technological potential, and they can be recovered for application as aromas by the food industry. 相似文献
106.
107.
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions
Monte LG Conceição FR Coutinho ML Seixas FK da Silva EF Vasconcellos FA deCastro LA Hartleben CP Dellagostin OA Aleixo JA 《Comparative immunology, microbiology and infectious diseases》2011,34(5):441-446
Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2–7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107 M−1 to 4 × 108 M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis. 相似文献
108.
Ramos CA Araújo FR Souza II Bacanelli G Luiz HL Russi LS Oliveira RH Soares CO Rosinha GM Alves LC 《Veterinary parasitology》2011,176(1):79-83
This paper reports a quantitative real-time polymerase chain reaction (q-PCR) based on the msa2c gene and standardized with Platinum SYBR Green/ROX for the detection of Babesia bovis in cattle. The msa2c q-PCR amplified a DNA fragment with average dissociation temperature of 77.41°C (± 0.25°C). No amplification was detected when DNA from B. bigemina, A. marginale or Bos taurus was used as the template. The detection limit of the msa2c q-PCR was 1000 copies per ml of blood sample, with a linear correlation between the number of msa2c copies and threshold cycle. The comparison between msa2c q-PCR and conventional PCR for cytochrome b revealed 88.8% agreement, with a Kappa index of 0.75. In the comparison between msa2c q-PCR and an enzyme-linked immunosorbent assay (ELISA) with semi-purified B. bovis antigen, agreement was 96.3% and the Kappa index was 0.91. The agreement between three tests was 85.8%. The msa2c q-PCR detected a higher number of positive cattle than conventional PCR in an enzootically stable area, but did not differ significantly from ELISA. No significant differences were detected between the three diagnostic tests with cattle from an enzootically unstable area. All animals raised on a tick-free facility were negative for B. bovis in the three tests. These results suggest that msa2c q-PCR is a useful test for the detection of B. bovis infection. 相似文献
109.
Luppi MM Luiz AP Coelho FM Malta Mde C Preis IS Ecco R da Fonseca FG Resende M 《Veterinary microbiology》2011,154(1-2):69-77
Psittacid herpesvirus (PsHV) was isolated from 41 birds kept in captivity in Belo Horizonte, Minas Gerais/Brazil using chicken embryo fibroblasts (CEF) cell cultures. For this study, leukocytes or cloacal swabs of live birds were used. Also, portions of liver, spleen or kidney from birds collected at necropsy were utilized for these tests. PCR tests confirmed the presence of PsHV in 100% of samples. Thirty-three of the PCR products were sequenced and the results disclosed a 99% and 100% identity when compared with other sequences PsHV-1 (AY372243.1 and AF261756.1), previously deposited in GenBank. In addition, histopathology was performed and 19 of the 29 birds contained random multifocal lymphoplasmacytic hepatitis with necrotic foci, suggestive of viral infection. Three samples were examined by electron microscopy to visualize the viral particles obtained from cell culture. The viral structures measured 269 nm in average, had envelopes with an icosahedral capsid and tegument, consistent with herpesvirus. Thus, a total of 41 isolates were obtained from PsHV cell cultivation in CEF, confirming the circulation of the virus between parrots kept in captivity in Belo Horizonte, and affirming the importance of further studies in this area. 相似文献
110.
Nesje, M., Flåøsyen, A. and Moe, L., 1997. Estimation of glomerular filtration rate in normal sheep by the disappearance of iohexol from serum. Veterinary Research Communications, 21 (1), 29-35. The aim of the study was to establish reference values for glomerular filtration rate (GFR) in healthy sheep from the clearance of iohexol in serum. Fifteen healthy sheep were tested twice with 14 to 21 days between tests. No side-effects were observed after iohexol injections and all the sheep were clinically normal during and after the study. The mean clearance of iohexol estimated by the two-compartment method was 1.8 ml/min per kg (95% CI = 1.6-2.0) in the first trial and 1.7 ml/min per kg (1.5-1.9) in the second trial. The mean GFRiohexol estimated by a one-compartment method was 1.9 ml/min per kg (1.7-2.2) in the first trial and 1.8 ml/min per kg (1.6-2.0) in the second. The GFR values were similar to those reported for the inulin method. The results indicate that the iohexol method is valid for estimating GFR in sheep, and it is easier to perform than the inulin method. 相似文献