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排序方式: 共有225条查询结果,搜索用时 31 毫秒
121.
Metabolic changes due to experimentally induced rupture of the bovine urinary bladder 总被引:1,自引:0,他引:1
D C Sockett A P Knight M J Fettman A R Kiehl J A Smith S M Arnold 《The Cornell veterinarian》1986,76(2):198-212
Metabolic changes were studied in the serum, saliva and peritoneal fluid of 5 healthy yearling feedlot steers after experimentally induced urinary bladder rupture. There were statistically significant decreases in serum, saliva and peritoneal fluid sodium and chloride values and significant increases in serum, saliva and peritoneal fluid urea nitrogen, creatinine and phosphorus values. Serum calcium, pH, bicarbonate, and base excess decreased significantly. Potassium did not change significantly in serum but did increase significantly in the saliva. The hemogram and peritoneal fluid analysis failed to provide clinicopathologic evidence of peritonitis. The average time of death or euthanasia after bladder rupture was 13.6 days with a range of 8-21 days. No single biochemical parameter could be identified which would allow prediction of the approaching time of death or duration of the disease process. There was no peritonitis at necropsy indicating that urine was not irritating to the bovine peritoneal cavity. Extracellular replacement fluid with or without sodium bicarbonate supplementation appeared to be the fluid of choice for correction of fluid and electrolyte imbalances in steers with ruptured urinary bladders. The ratio between serum and peritoneal fluid creatinine concentrations appears to be valuable for the clinical diagnosis of ruptured urinary bladders in steers. 相似文献
122.
JL Albarracín T Mogas MJ Palomo A Peña T Rigau JE Rodríguez-Gil 《Reproduction in domestic animals》2004,39(3):129-135
Incubation of dog spermatozoa in a medium without glucose and in the presence of lactate and pyruvate (l-CCM) for 4 h at 38.5 degrees C in a 5% CO(2) atmosphere induced in vitro capacitation of these cells. This was verified after the combined specific capacitation-like changes in percentages of viability and altered acrosomes, motility characteristics, sperm location of reactivity against Pisum sativum, Arachis hypogaea and Helix pomatia lectins and the tyrosine phosphorylation pattern. Furthermore, a feasible acrosome reaction (AR) was induced when spermatozoa incubated in l-CCM for 4 h were further co-incubated for 1 h with canine oocytes. This was demonstrated by AR-like changes in percentages of viability, altered acrosomes, motility characteristics and sperm location of reactivity against P. sativum, A. hypogaea and H. pomatia lectins. All these results clearly indicate that in vitro capacitation, and subsequent AR, can be feasibly achieved without the presence of sugars. This ability can be related to the specific characteristics of energy-metabolism regulation reported in dog spermatozoa. 相似文献
123.
The present study was carried out to study the effect of different maturation media on embryo development of heifer oocytes and on their glutathione (GSH) synthesis during in vitro maturation (IVM). Immature heifer oocytes were matured in parallel in one of four maturation media: (i) Tissue Culture Medium (TCM)-199 supplemented with 10 ng/ml of epidermal growth factor (EGF); (i) TCM-199 supplemented with 10 ng/ml of EGF plus 1 microg/ml of FSH; (iii) TCM-199 supplemented with 10% of foetal bovine serum (FBS) and (iv) TCM-199 supplemented with 10% of FBS plus 1 microg/ml of FSH. Cow oocytes were used as control and were matured in TCM-199 supplemented with 10 ng/ml of EGF. No differences were observed in blastocyst rate among the different heifer oocyte groups (8.8, 7.5. 8.4 and 6.8%, respectively) however, the percentage of blastocysts obtained from cow oocytes was significantly higher (30%; p < 0.01) than those obtained from heifer oocytes. De novo GSH synthesis during oocyte maturation of heifer and cow oocytes was detected. No significant differences in intracytoplasmic GSH levels were observed among the experimental heifer oocyte groups or between heifer and cow oocytes both before and after IVM. In conclusion, the blastocyst yield obtained from heifer oocytes was lower than that from cow oocytes and this fact could not be explained by significant differences in intracytoplasmic GSH contents of oocytes before or after IVM. 相似文献
124.
K W Weingand M J Fettman R W Phillips M S Hand 《American journal of veterinary research》1986,47(2):441-446
Four healthy, fully conscious, 50- to 80-kg Yucatan miniature swine were fitted with carotid artery and jugular, portal, and hepatic vein catheters and hepatic artery and portal vein flow cuffs to determine transhepatic kinetics and insulin secretion. Three days later, after a 3-hour control period, the pigs were given IV sodium dichloroacetate (30 mg/kg of body weight as a bolus and 30 mg/kg/hr, as an infusion) for 6 hours. Arterial lactate concentrations decreased during the dichloroacetate infusion, but pyruvate concentrations were unaltered. The whole body rate of appearance and disappearance of glucose decreased, but plasma glucose concentrations did not change markedly. Limb skin surface temperatures increased, indicating a peripheral vasodilatory effect. Dichloroacetate had little effect on mean arterial pressure and hepatosplanchnic blood flow. Dichloroacetate may be effective as a hypolactatemic agent for lactic acidosis in pigs. 相似文献
125.
Maguire PJ Fettman MJ Smith MO Greco DS Turner AS Walton JA Ogilvie GK 《Journal of the American Veterinary Medical Association》2000,217(6):847-852
OBJECTIVE: To determine effects of various diets on the pharmacokinetics of phenobarbital and the interactive effects of changes in body composition and metabolic rate. DESIGN: Prospective study. ANIMALS: 27 healthy sexually intact adult female Beagles. PROCEDURE: Pharmacokinetic studies of phenobarbital were performed before and 2 months after dogs were fed 1 of 3 diets (group 1, maintenance diet; group 2, protein-restricted diet; group 3, fat- and protein-restricted diet) and treated with phenobarbital (approx 3 mg/kg [1.4 mg/lb] of body weight, p.o., q 12 h). Pharmacokinetic studies involved administering phenobarbital (15 mg/kg [6.8 mg/lb], i.v.) and collecting blood samples at specific intervals for 240 hours. Effects of diet and time were determined by repeated-measures ANOVA. RESULTS: Volume of distribution, mean residence time, and half-life (t1/2) of phenobarbital significantly decreased, whereas clearance rate and elimination rate significantly increased with time in all groups. Dietary protein or fat restriction induced significantly greater changes: t1/2 (hours) was lower in groups 2 (mean +/- SD; 25.9 +/- 6.10 hours) and 3 (24.0 +/- 4.70) than in group 1 (32.9 +/- 5.20). Phenobarbital clearance rate (ml/kg/min) was significantly higher in group 3 (0.22 +/- 0.05 ml/kg/min) than in groups 1 (0.17 +/- 0.03) or 2 (0.18 +/- 0.03). Induction of serum alkaline phosphatase activity (U/L) was greater in groups 2 (192.4 +/- 47.5 U/L) and 3 (202.0 +/- 98.2) than in group 1 (125.0 +/- 47.5). CONCLUSIONS AND CLINICAL RELEVANCE: Clinically important differences between diet groups were observed regarding pharmacokinetics of phenobarbital, changes in CBC and serum biochemical variables, and body composition. Drug dosage must be reevaluated if a dog's diet, body weight, or body composition changes during treatment. Changes in blood variables that may indicate liver toxicosis caused by phenobarbital may be amplified by diet-drug interactions. 相似文献
126.
Leibman NF Lana SE Hansen RA Powers BE Fettman MJ Withrow SJ Ogilvie GK 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(6):583-586
Presence of matrix metalloproteinases has been associated with tumor invasion and metastasis in human neoplasia. The presence of matrix metalloproteinase 2 and matrix metalloproteinase 9 was determined in canine mast cell tumor tissue and normal stromal tissue from 24 dogs with spontaneously occurring cutaneous mast cell tumors. Seventeen of the mast cell tumors were of histologic grade 2, and 7 were of histologic grade 3. Gelatin zymography and computer assisted densitometry image analysis were used to quantify matrix metalloproteinase concentration. Bands from canine tissues migrated in the same location as human proenzyme and active enzyme matrix metalloproteinase 2 and matrix metalloproteinase 9 standards. A semiquantitative value for each patient sample was obtained by comparing the optical assessment density of each unknown band to the optical density of the human standard. The presence of matrix metalloproteinase 2 and matrix metalloproteinase 9 in histologic grade 2 mast cell tumors and histologic grade 3 mast cell tumors was compared, as was presence of matrix metalloproteinases in tumor and stromal tissue. There was dramatically more proenzyme matrix metalloproteinase 9 activity in histologic grade 3 mast cell tumors when compared to grade 2 tumors (P = .03). There was also dramatically more active enzyme matrix metalloproteinase 2 and active enzyme matrix metalloproteinase 9 activity in tumor tissue compared to stromal tissue (P = .02, P < .0001). This study demonstrates that the proenzyme and active enzyme forms of matrix metalloproteinase 2 and matrix metalloproteinase 9 are present in canine mast cell tumors. This appears to be related to the degree of histologic malignancy, although histologic grade 1 tumors were not evaluated. 相似文献
127.
128.
MK Gagan LK Ayliffe D Hopley JA Cali GE Mortimer J Chappell MT McCulloch MJ Head 《Science (New York, N.Y.)》1998,279(5353):1014-1018
Skeletal Sr/Ca and 18O/16O ratios in corals from the Great Barrier Reef, Australia, indicate that the tropical ocean surface approximately 5350 years ago was 1 degrees C warmer and enriched in 18O by 0.5 per mil relative to modern seawater. The results suggest that the temperature increase enhanced the evaporative enrichment of 18O in seawater. Transport of part of the additional atmospheric water vapor to extratropical latitudes may have sustained the 18O/16O anomaly. The reduced glacial-Holocene shift in seawater 18O/16O ratio produced by the mid-Holocene 18O enrichment may help to reconcile the different temperature histories for the last deglaciation given by coral Sr/Ca thermometry and foraminiferal oxygen-isotope records. 相似文献
129.
KW Oum MJ Lakin DO DeHaan T Brauers BJ Finlayson-Pitts 《Science (New York, N.Y.)》1998,279(5347):74-77
Halogen atoms from the reactions of sea-salt particles may play a significant role in the marine boundary layer. Reactions of sodium chloride, the major component of sea-salt particles, with nitrogen oxides generate chlorine atom precursors. However, recent studies suggest there is an additional source of chlorine in the marine troposphere. This study shows that molecular chlorine is generated from the photolysis of ozone in the presence of sea-salt particles above their deliquescence point; this process may also occur in the ocean surface layer. Given the global distribution of ozone, this process may provide a global source of chlorine. 相似文献
130.
Mechanism of the Zonal Displacements of the Pacific Warm Pool: Implications for ENSO 总被引:7,自引:0,他引:7
J Picaut M Ioualalen C Menkes T Delcroix MJ McPhaden 《Science (New York, N.Y.)》1996,274(5292):1486-1489
The western equatorial Pacific warm pool is subject to strong east-west migrations on interannual time scales in phase with the Southern Oscillation Index. The dominance of surface zonal advection in this migration is demonstrated with four different current data sets and three ocean models. The eastward advection of warm and less saline water from the western Pacific together with the westward advection of cold and more saline water from the central-eastern Pacific induces a convergence of water masses at the eastern edge of the warm pool and a well-defined salinity front. The location of this convergence is zonally displaced in association with El Nino-La Nina wind-driven surface current variations. These advective processes and water-mass convergences have significant implications for understanding and simulating coupled ocean-atmosphere interactions associated with El Nino-Southern Oscillation (ENSO). 相似文献