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481.
Ferguson AD Chakraborty R Smith BS Esser L van der Helm D Deisenhofer J 《Science (New York, N.Y.)》2002,295(5560):1715-1719
Siderophore-mediated acquisition systems facilitate iron uptake. We present the crystallographic structure of the integral outer membrane receptor FecA from Escherichia coli with and without ferric citrate at 2.5 and 2.0 angstrom resolution. FecA is composed of three distinct domains: the barrel, plug, and NH2-terminal extension. Binding of ferric citrate triggers a conformational change of the extracellular loops that close the external pocket of FecA. Ligand-induced allosteric transitions are propagated through the outer membrane by the plug domain, signaling the occupancy of the receptor in the periplasm. These data establish the structural basis of gating for receptors dependent on the cytoplasmic membrane protein TonB. By compiling available data for this family of receptors, we propose a mechanism for the energy-dependent transport of siderophores. 相似文献
482.
Ferguson ES 《Science (New York, N.Y.)》1977,197(4306):827-836
483.
Gregory D Roach Kathryn J Reid Sally Ferguson Drew Dawson 《Journal of circadian rhythms》2006,4(1):8-5
Background
The aim of the study was to examine the role that melatonin production plays in the regulation of sleep consolidation in a population of shiftworkers working and sleeping in their natural environments. 相似文献484.
485.
Influence of class IV laser therapy on the outcomes of tibial plateau leveling osteotomy in dogs
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486.
Robert L. McLaughlin Moira M. Ferguson David L. G. Noakes 《Fish physiology and biochemistry》1995,14(6):459-469
To be useful as short-term indices of nutritional status when food availability varies, wet weight-at-length and tissue concentrations of nucleic acids and protein must covary closely with a fish's recent feeding history. We measured changes in these indices for young brook trout (Salvelinus fontinalis) (fork length: 20–34 mm) reared under alternating, 4 to 5 d periods of food provisioning and food deprivation. Weight-at-length corresponded closely with current feeding conditions, being higher when the trout were fed than when they were deprived. Concentrations of RNA and protein, however, did not correspond closely with current feeding conditions. Instead, there were significant carry-over effects whereby responses to feeding conditions experienced in one treatment period were not exhibited until the following treatment period. Food provisioning had positive carry-over effects on concentrations of RNA and protein while food deprivation had negative carry-over effects. Consequently, food-deprived trout sometimes had higher concentrations of RNA and protein than well fed trout. Since wild fish may experience short-term fluctuations in food availability, lagged responses in concentrations of nucleic acids or protein like those reported here could hamper attempts to use these biochemical measures to assess the nutritional status of juvenile fish in the field.Author to whom all correspondence should be addressed. 相似文献