BACTERIAL ULCERATIVE DERMATITIS IN A PIRANHA (PYGOCENTRUS NATTERERI) FED WITH FRESH FISH DIET

A red-bellied piranha (Pygocentrus nattereri), kept as a pet by a private owner, was presented for multifocal ulcerative lesions. Lesion biopsies were collected under general anesthesia and submitted for histopathologic and microbiologic examination. Histological examination revealed a bacterial necrotizing ulcerative dermatitis. From cultural analysis of the biopsy, Aeromonas hydrophila and Edwardsiella ictaluri were isolated. Multidrug resistance was observed in both isolates, except for neomycin and ceftazidime. Intramuscular injections of ceftazidime 22 mg/kg were administered until complete healing of the wound, except for small dermal scars that remained following treatment. Given that the piranha was maintained alone since it was purchased, except for occasional feedings with fresh nonfrozen goldfish, horizontal transmission of the infection from the prey species was considered the source of infection.     

幼龄仔猪PEDV感染肠道损伤模型的建立

试验旨在建立猪流行性腹泻病毒（PEDV）感染幼龄仔猪肠道损伤模型。试验选用16头7日龄健康幼龄仔猪（杜×长×大）,随机分为两个处理组：对照组和PEDV组,每个处理8个重复,每个重复1头猪。试验期为10 d,试验期间两个处理组饲喂相同的基础日粮。于试验第7天晚上对PEDV组仔猪口腔灌服PEDV病毒（剂量为10^4.5 TCID₅₀）,对照组灌服等量的生理盐水。于试验第10天早上空腹灌服D-木糖（0.1 g/kg体重）,1 h后前腔静脉采血,屠宰取样,取十二指肠、空肠、回肠等组织样品,测定平均日增重（ADG）、腹泻率（DR）、肠道形态结构、肠道黏膜损伤相关基因mRNA水平。试验结果表明：①PEDV感染显著降低了仔猪ADG（P<0.05）,极显著提高了仔猪腹泻率（P<0.01）;②PEDV感染极显著降低了血浆D-木糖含量、空肠和回肠绒毛高度、十二指肠、空肠和回肠绒毛高度与隐窝深度的比值（P<0.01）,显著提高了十二指肠和空肠隐窝深度（P<0.05）;③PEDV感染极显著提高了仔猪空肠黏膜PEDV M基因的相对表达量（P<0.01）,极显著降低了肠绒毛蛋白（villin）和肠型脂肪酸结合蛋白（i-FABP）基因的相对表达量（P<0.01）。以上结果表明,口腔灌服PEDV可以成功诱导建立幼龄仔猪肠道损伤模型。     

郁金香离体快繁技术研究

以2个郁金香品种"阿波罗"和"琳.马克"为试材,用不同时期、不同部位、不同类型的郁金香鳞片作为外植体,以MS为基本培养基,分别添加不同质量浓度的6-BA、NAA、IAA和活性炭,研究不同外植体以及不同培养基对小鳞茎诱导的影响。结果表明:经春化处理后的鳞片作为外植体诱导小鳞茎的效果最好,鳞片部位以中层最适宜诱导小鳞茎;以带鳞茎盘的双鳞片作诱导小鳞茎的外植体效果最好;最适宜"阿波罗"小鳞茎诱导的激素组合为MS+6-BA 2mg/L+NAA 2mg/L+IAA 0.3mg/L;最适宜"琳.马克"小鳞茎诱导的激素组合为MS+6-BA 2mg/L+NAA 2mg/L+IAA 0.1mg/L;活性炭对小鳞茎的诱导有促进作用;最适宜的暗处理时间为7d。     

CryⅢA 基因植物表达载体构建及马铃薯遗传转化简

 利用Ｉｎ-Ｆｕｓｉｏｎ技术将酶切位点不匹配、目的基因内部含载体酶切位点的CRY  ⅢA 基因和植物表达载体ｐＢＩ１２１相连接，分别成功构建了组成型启动子ＣａＭＶ３５Ｓ和马铃薯茎叶特异表达启动子ＳＴ-ＬＳ１驱动的CRY  ⅢA 基因植物表达载体。利用冻融法将２个重组质粒转入根癌农杆菌ＬＢＡ４４０４，转化马铃薯栽培品种陇薯３号和甘农薯２号获得了转化植株，经卡那霉素筛选和ＰＣＲ 测，共有１０株阳性植株CRY  ⅢA 基因成功整合到马铃薯基因组中。经实时荧光定量ＰＣＲ 检测表明，CRY  ⅢA 基因在ＣａＭＶ３５Ｓ驱动的CRY  ⅢA 转基因植株的根、茎、叶中均有表达，且在叶中表达量较高，茎和根次之；在马铃薯茎叶特异表达启动子ＳＴ-ＬＳ１驱动的CRY  ⅢA 转基因植株中，在根中未见表达，仅在茎、叶中表达，且在叶中表达量较高。     

Monitoring managed forest structure at the compartment-level under different silvicultural heritages: An exploratory data analysis in Italy

ABSTRACT

The more and more diffused multifunctional role addressed nowadays to public forests, calls for targeted analysis aimed at highlighting the overall outcome of different practices implemented on the same forest compartment, according to the locally prevailing function. This study was carried out in four Italian beech forests across a latitudinal gradient representative of multiple management history, stand structure, and dominant stand age. We analyze forest structure at the compartment scale before and after silvicultural practices. We aim to explore relationships and similarities between 10 stand attributes (mensurational and structural variables) to identify relevant indicators for the monitoring and management of forest ecosystems. Results indicate changing patterns of correlation and similarity among mensurational variables following practice implementation. A sensitivity gradient to silvicultural practice was finally identified within the four sites investigated as a result of the diverging stand structure. Our approach suggests a way and provides an insight for the design of adaptive forestry management practices required to meet environmental targets, in addition to the already acknowledged supply of primary goods and services.     

基于传感技术汽车智能双闪灯警示装置

基于传感技术汽车智能双闪灯警示装置适用于无红绿灯人行道、十字路口、急拐弯路口或存在驾驶观察盲区的危险区域等多种路况下,对人、动物的双闪灯警示作用.     

浅析安徽宁国红豆杉特色林业生态园规划

随着人们生活水平以及生态保护意识的提高,在生态造园的理念下,特色林业生态园作为塑造优美的森林环境、高效的林业产业与特色的生态文化和谐统一的典范,其发展前景越来越广阔。本文以安徽宁国红豆杉生态园规划为例,从区域概况、规划原则及目标定位、分区规划及项目规划、景观规划、产业链规划等几个方面进行探索探究,探讨了如何依托特色林业资源,发展观光林业,推动生态旅游,以期对特色林业生态园的规划设计起到借鉴作用。