Genetic variation,heritability and correlations of agro-morphological traits in tall fescue (<Emphasis Type="Italic">Festuca arundinacea</Emphasis> Schreb.)

To study the genetic variation, heritability and genetic correlations of some agro-morphological traits in tall fescue, 25 parents from a genetically broad-base germplasm were polycrossed and their respective half-sib families were generated. Clonally propagated parents and their half-sib families were grown as spaced single plants using a randomized complete-block design with three replications, and observed for seven traits in 2006 and 2007. The estimates of broad-sense heritability were moderate to high for the traits studied. Narrow-sense heritability estimates from analyses of progenies and from regression of half-sib (HS) progenies on parents suggested that genetic variation for these traits was largely controlled by additive gene action. Association of dry matter yield (DMY) with plant height, number of fertile shoot, curbs width and spring growth was positive and significant. With the exception of number of days to pollination, correlation coefficients of the traits between the parents and offspring were not significant. Based on parent-offspring regression, genetic gain from selection for DMY was high, demonstrating genetic potential for improving this trait. Overall, there was high genetic variation and moderate heritability for most traits in the tall fescue populations evaluated. In conclusion, to improve herbage yield, selection would be more effective based on forage yield components.     

Canopy cover or remotely sensed vegetation index,explanatory variables of above-ground biomass in an arid rangeland,Iran

Estimation of above-ground biomass is vital for understanding ecological processes. Since direct measurement of above-ground biomass is destructive, time consuming and labor intensive, canopy cover can be considered as a predictor if a significant correlation between the two variables exists. In this study, relationship between canopy cover and above-ground biomass was investigated by a general linear regression model. To do so, canopy cover and above-ground biomass were measured at 5 sub-life forms(defined as life forms grouped in the same height classes) using 380 quadrats, which is systematic-randomly laid out along a 10-km transect, during four sampling periods(May, June, August, and September) in an arid rangeland of Marjan, Iran. To reveal whether obtained canopy cover and above-ground biomass of different sampling periods can be lumped together or not, we applied a general linear model(GLM). In this model, above-ground biomass was considered as a dependent or response variable, canopy cover as an independent covariate or predictor factor and sub-life forms as well as sampling periods as fixed factors. Moreover, we compared the estimated above-ground biomass derived from remotely sensed images of Landsat-8 using NDVI(normalized difference vegetation index), after finding the best regression line between predictor(measured canopy cover in the field) and response variable(above-ground biomass) to test the robustness of the induced model. Results show that above-ground biomass(response variable) of all vegetative forms and periods can be accurately predicted by canopy cover(predictor), although sub-life forms and sampling periods significantly affect the results. The best regression fit was found for short forbs in September and shrubs in May, June and August with R~2 values of 0.96, 0.93 and 0.91, respectively, whilst the least significant was found for short grasses in June, tall grasses in August and tall forbs in June with R~2 values of 0.71, 0.73 and 0.75, respectively. Even though the estimated above-ground biomass by NDVI is also convincing(R~2=0.57), the canopy cover is a more reliable predictor of above-ground biomass due to the higher R~2 values(from 0.75 to 0.96). We conclude that canopy cover can be regarded as a reliable predictor of above-ground biomass if sub-life forms and sampling periods(during growing season) are taken into account. Since,(1) plant canopy cover is not distinguishable by remotely sensed images at the sub-life form level, especially in sparse vegetation of arid and semi-arid regions, and(2) remotely sensed-based prediction of above-ground biomass shows a less significant relationship(R~2=0.57) than that of canopy cover(R~2 ranging from 0.75 to 0.96), which suggests estimating of plant biomass by canopy cover instead of cut and weighting method is highly recommended. Furthermore, this fast, nondestructive and robust method that does not endanger rare species, gives a trustworthy prediction of above-ground biomass in arid rangelands.     

Evaluation of the learning curve for natural orifice transluminal endoscopic surgery: bilateral ovariectomy in dogs

Objective: Study the learning curve for canine Natural Orifice Transluminal Endoscopic Surgery (NOTES) ovariectomy by evaluating operative times and complications. Study Design: Preclinical research study. Animals: Adult female dogs (n=20). Methods: NOTES ovariectomy procedures were performed as follows: Feasibility Group 1 (n=5), Feasibility Group 2 (n=5), and Early Clinical Group 3 (n=10). Six steps of the procedure were identified, timed separately, and the overall time was recorded from introduction to removal of the endoscope. Complications were recorded. Repeated measures analysis of variance using ranked data compared the effect of group (3 levels) on the time for each step. Nonlinear regression using an exponential model with nonzero asymptote was used to model the operative time–procedure number relationship. Results: Overall median operative time was significantly longer for Group 1 (195 minutes; range, 160–265 minutes) than Group 2 (108 minutes; range, 81–148 minutes; P=.048) and Group 3 (77 minutes; range, 41–136 minutes; P=.0008). The estimated asymptotic operative time was 71 minutes (95% confidence interval, 41–100 minutes); this time was reached after 10 procedures. Gastric cleansing and removing the left ovary took significantly longer in Group 1 than in Group 2 or 3. Operative complications included incomplete ovarian excision, dropping an ovary during retrieval, and conversion to an open procedure. No intraoperative complications occurred in Group 3. Conclusion: NOTES procedures result in longer operative times in the early part of the learning curve and require considerable experience before reaching proficiency.     

Gelatin from Saithe (Pollachius virens) Skin: Biochemical Characterization and Oxidative Stability in O/W Emulsions

This study performed the extraction of gelatin from saithe (Pollachius virens) skin and compared it to commercial marine gelatin. As a first stage, we investigated the physicochemical and biochemical properties of the gelatin. SDS-PAGE analysis revealed the presence of α-chains, β-chains, and other high-molecular-weight aggregates. DSC thermograms showed typical gelatin behavior, while the FTIR spectra were mainly situated in the amide band region (amide A, amide B, amide I, amide II, and amide III). In the second stage, we produced O/W emulsions and analyzed their physical and oxidative stability over 9 days. Oil droplets stabilized with the gelatins obtained from saithe fish skin had a size of ~500 nm and a ζ-potential ~+25 mV, which is comparable to oil droplets stabilized with commercial gelatin products. Moreover, the oxidative stability of the emulsions stabilized with gelatin from saithe fish skin showed promising results in terms of preventing the formation of some volatile compounds towards the end of the storage period compared to when using the commercial gelatins. This study indicates the potential application of fish skin gelatin in the fields of food and cosmetics, as well as suggesting that further investigations of their techno-functional properties.     

Quantitative study of poplar plantations in three Iranian provinces

Fast growing poplar species enjoy a highly favored position in Iran＇s forest product industries. Howeve~ information on poplar plantations, such as areas, growing stock and harvest volumes, are largely obtained by now scientific means and poorly executed methods. A few studies have been conducted to obtain data on the capacit,. of poplar plantations, their extent, existing growing stock, distribution and species choice in three provinces, i.e. Western Azerbaijan, Kurdistan and Hamedan, with relatively well developed management systems. We opted for cluster method, a standard sampling method for conducting similar investigations, consisting of two phases. In the firs phase we collected library information and conducted half-open interviews with villagers. In the second phase fiel~ measurements in the villages of these three provinces were carried out. Information from field measurements on growin! stock, cultivated areas, dominant species were used to estimate volumes by way of volume and weight tables. Result~ obtained from the present study indicate that the average annual volume of timber harvested in the three province~ was 697,723 m~, with an average sampling error of 22.7 per cent. This annual volume of poplar timber harvested fron the three provinces was estimated to amount to about 25 per cent of overall harvest; at that rate, the overall annu~ utilization potential of poplar plantations will be 10 million m3, which constitutes a reliable resource of raw timber for us~ in wood and paper industries.     

Morphology and genetic diversity of Marrubium cuneatum Russell and M. parviflorum Fisch. & C.A. Mey

Genetic Resources and Crop Evolution - Marrubium parviflorum Fisch. &amp; C.A. Mey. and Marrubium cuneatum Russell, are naturally distributed in various parts of Iran. It is difficult to...     

Evaluation of Salinity Effects on Ionic Balance and Compatible Solute Contents in Nine Grape (Vitis L.) Genotypes

The salinity tolerance of nine grape genotypes was studied. Salinity was applied as nutrient solutions containing 0, 25, 50, and 100 mM sodium chloride (NaCl) for two weeks. Growth was significantly reduced by salinity, whereas chloride (Cl^?) and sodium (Na⁺) contents increased. Sodium ion accumulation exceeded that of Cl^? in all treatments. Shirazi and H₆ had higher and lower Cl^? concentrations in their lamina than others. There were significant positive correlations (P < 0.01) between Cl^? and Na⁺ and negative correlation between Na⁺ and potassium (K⁺) in roots and laminas of all genotypes. Soluble sugars, proline, and glycine betaine contents increased in laminas of all of the genotypes with moderate salinity. There were positive correlations (P < 0.01) between lamina and root Na⁺ and Cl^? contents and compatible solutes in all genotypes. Overall results revealed that unlike Shirazi with higher Na⁺ and Cl^? accumulation in shoot, H₆ showed a higher capacity to restrict Na⁺ and Cl^? transport to shoot.     

Effects of green synthesized zinc and copper nano-fertilizers on the morphological and biochemical attributes of basil plant

Abstract

In the present study, the green synthesis of Zn and Cu nanoparticles (NPs) was carried out via Zn and Cu ions reduction during their exposure to basil extract. The shape, size and chemical identity of the Zn and Cu NPs were determined using SEM and XRD analysis. To investigate the effects of the Zn and Cu NPs on the morphological and biochemical traits of basil plant, an experiment was conducted as a factorial based on completely randomized design with 4 levels of Zn NPs (0, 1000, 2000, and 4000?ppm) and 4 levels of Cu NPs (0, 1000, 2000, and 4000?ppm). Based on the results, nutrient treatments, especially 4000?ppm Zn NPs + 2000?ppm Cu NPs caused a significant increase in most morphological parameters. The application of the Zn and Cu NPs significantly affected concentrations of chlorophyll a, chlorophyll b, total chlorophyll and carotenoid in the leaves of basil plants. The highest total phenolic and flavonoid content was obtained for 4000?ppm Zn NPs + 2000?ppm Cu NPs treatment. Plants treated with 4000?ppm Zn NPs + 0?ppm Cu NPs showed the highest antioxidant activity followed by 4000?ppm Zn NPs + 2000?ppm Cu NPs and 2000?ppm Zn NPs + 0?ppm Cu NPs. It can be concluded that foliar application of the Zn and Cu NPs is necessary for obtaining better quantity and quality in basil.