A Comparative Study on the Flocculation of Silica and China Clay with Chitosan and Synthetic Polyelectrolytes

Flocculation is still one of the most important and efficient processes for water treatment. However, most industrial processes, such as in water treatment plants, still use huge amounts of synthetic polyelectrolytes for the flocculation process. Here we compare the flocculation of two different suspended particles, i.e., silica particles and china clay, with the biopolymer chitosan and two common strong synthetic polyelectrolytes. As a flocculant, chitosan featured a minimum uptake rate of 0.05 mg/g for silica and 1.8 mg/g for china clay. Polydiallyldimethylammonium chloride (PDADMAC) for comparison possessed a minimum uptake rate of 0.05 mg/g for silica and 2.2 mg/g for china clay. Chitosan as an environmentally friendly biopolymer competes with the synthetic polyelectrolytes and thus represents a beneficial economic alternative to synthetic flocculants.     

A Preliminary Study of an Inertial Sensor-based Method for the Assessment of Human Pelvis Kinematics in Dressage Riding

Kinematic studies of horse and rider often suffer from their limitation to laboratory conditions because of video-based measurement techniques. To date, there are no suitable sensor-based methods published for the assessment of the rider's pelvis. The aim of this study was to reveal the possibilities and limitations of inertial sensors to assess the motion of the rider's pelvis in walk, trot, and canter, with particular attention to repeatability. Two female riders rode the same horse in repeated trials wearing an inertial sensor attached to their pelvis. Both riders were tested in walk, sitting trot, rising trot, and canter. Gait-dependent and interindividual characteristics of pelvis rotation in dressage riding under field conditions could be demonstrated. The possibility of obtaining kinematic data of horse and rider under field conditions using inertial sensors seems to be promising not only for researchers and horsemen but also for objectifying progress in therapeutic riding.     

Assessment of the Salmonella burden in slaughter pigs through the results of meat-juice-ELISA.]

During a study on the salmonella infection rate in slaughter pigs, bacteriological results were collected from faecal samples and intestinal lymphnodes, and serological results were acquired from meat-juice-ELISA. Serological findings and resulting serological parameters were analysed for their capacity to provide an indication of the salmonella burden in a given lot of slaughter pigs or in the farm of origin. The arithmetic mean antibody concentration of a lot of slaughter pigs or a piggery, or the number of animals reacting above 30% according to the Danish standard, or the number of animals reacting above 40% according to the Danish standard are all three equally suited to differentiate between a low and a high level of infection. The mean antibody concentration was found to be the best parameter for epidemiological studies that compare the serological status between herds or investigate the development of antibodies within a given piggery.     

Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells

ABSTRACT: Mycobacterial lipids have long been known to modulate the function of a variety of cells of the innate immune system. Here, we report the extraction and characterisation of polar and apolar free lipids from Mycobacterium bovis AF 2122/97 and identify the major lipids present in these fractions. Lipids found included trehalose dimycolate (TDM) and trehalose monomycolate (TMM), the apolar phthiocerol dimycocersates (PDIMs), triacyl glycerol (TAG), pentacyl trehalose (PAT), phenolic glycolipid (PGL), and mono-mycolyl glycerol (MMG). Polar lipids identified included glucose monomycolate (GMM), diphosphatidyl glycerol (DPG), phenylethanolamine (PE) and a range of mono- and di-acylated phosphatidyl inositol mannosides (PIMs). These lipid fractions are capable of altering the cytokine profile produced by fresh and cultured bovine monocytes as well as monocyte derived dendritic cells. Significant increases in the production of IL-10, IL-12, MIP-1β, TNFα and IL-6 were seen after exposure of antigen presenting cells to the polar lipid fraction. Phenotypic characterisation of the cells was performed by flow cytometry and significant decreases in the expression of MHCII, CD86 and CD1b were found after exposure to the polar lipid fraction. Polar lipids also significantly increased the levels of CD40 expressed by monocytes and cultured monocytes but no effect was seen on the constitutively high expression of CD40 on MDDC or on the levels of CD80 expressed by any of the cells. Finally, the capacity of polar fraction treated cells to stimulate alloreactive lymphocytes was assessed. Significant reduction in proliferative activity was seen after stimulation of PBMC by polar fraction treated cultured monocytes whilst no effect was seen after lipid treatment of MDDC. These data demonstrate that pathogenic mycobacterial polar lipids may significantly hamper the ability of the host APCs to induce an appropriate immune response to an invading pathogen.     

Response of osteoclasts to titanium surfaces with increasing surface roughness: an in vitro study

Osteoclasts are responsible for bone resorption and implant surface roughness promotes osseointegration. However, little is known about the effect of roughness on osteoclast activity. This study aims at the characterization of osteoclastic response to surface roughness. The number of osteoclasts, the tartrate-resistant acid phosphatase and matrix metalloproteinase (MMP) activities, the cell morphology and the actin-ring formation were examined on smooth (TS), acid-etched (TA) and sandblasted acid-etched (TLA) titanium and on native bone. Cell morphology was comparable on TA, TLA and bone, actin rings being similar in size on TLA and bone, but smaller on TA and virtually absent on TS. Gelatin zymography revealed increased proMMP-9 expression on TA, TLA, and bone compared to TS. In general, osteoclasts show similar characteristics on rough titanium surfaces and on bone, but reduced activity on smooth titanium surfaces. These results offer some insight into the involvement of osteoclasts in remodeling processes around implant surfaces.